Mouse FPRP/PTGFRN Antibody

Catalog #: AF4495
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4495
AF4495-SP

Detection of FPRP/PTGFRN in 3T3‑L1 Mouse Cell Line by Flow Cytometry.

1 Image

All FPRP/PTGFRN Antibodies

Product Details

Citations (1)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse FPRP/PTGFRN Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse FPRP/PTGFRN in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse FPRP/PTGFRN
Arg22-Pro832
Accession # NP_035327

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse FPRP/PTGFRN

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of FPRP/PTGFRN in 3T3‑L1 Mouse Cell Line by Flow Cytometry. 3T3-L1 mouse embryonic fibroblast adipose-like cell line was stained with Sheep Anti-Mouse FPRP/PTGFRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4495, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Northern-Lights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FPRP/PTGFRN

FPRP (F_{2 alpha} Prostaglandin receptor Regulatory Protein; also CD315, EWI-F and CD9 partner-1/P-1) is a 130 kDa type I transmembrane (TM) glycoprotein member of the EWI subfamily, Ig superfamily of molecules. It associates with the 7-TM prostaglandin F_{2 alpha} receptor, inhibiting its activity. It also forms complexes with 4-TM CD9 and CD81, linking these molecules to the intracellular actin cytoskeleton. Mature mouse FPRP is 858 amino acids (aa) in length. Its extracellular domain (ECD) contains six C2-type Ig-like domains (aa 22‑813) plus a Pro-Thr-Glu-Leu ER retention signal. The ECD of mouse FPRP (aa 22‑832) shares 89% and 98% aa identity with the ECD of human and rat FPRP, respectively.

Long Name

Prostaglandin F2-alpha Receptor Regulatory Protein

Entrez Gene IDs

5738 (Human); 19221 (Mouse); 29602 (Rat)

Alternate Names

CD315; CD9P-1; FPRP; PTGFRN

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Mouse FPRP/PTGFRN Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1