The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified to date. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1-4). Mouse Galectin-1, also known as beta-galactoside-binding lectin L-14-I, lactose-binding lectin 1, S-Lac lectin 1, galaptin and 14 kDa lectin, is a monomeric or homodimeric prototype galectin that is expressed in a variety of cells and tissues including muscle, heart, lymph nodes, spleen, thymus, macrophages, B cells, T cells, dendritic cells, and tumor cells. It preferentially binds laminin, fibronectin, 90K/Mac-2BP, CD45, CD43, CD7, CD2, CD3, and ganglioside GM1. Galectin-1 modulates cell growth, proliferation and differentiation, either positively or negatively, depending on the cell type and activation status. It controls cell survival by inducing apoptosis of activated T cells and immature thymocytes. It modulates cytokine secretion by inducing Th2 type cytokines and inhibiting pro-inflammatory cytokine production. Galectin-1 can also modulate cell-cell as well as cell-matrix interactions and depending on the cell type and developmental stage, promote cell attachment or detachment. Galectin-1 has immunosuppressive and anti-inflammatory properties and has been shown to suppress acute and chronic inflammation and autoimmunity. Mouse and human Galectin-1 share about 88% amino acid sequence similarity (1-5).
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Label
Antibody Source
Product Specifications
Immunogen
Ala2-Glu135
Accession # P16045
Specificity
Clonality
Host
Isotype
Scientific Data Images for Mouse Galectin‑1 Antibody
Detection of Mouse Galectin‑1 by Simple WesternTM.
Simple Western lane view shows lysates of C2C12 mouse myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin‑1 at approximately 18 kDa (as indicated) using 50 µg/mL of Rat Anti-Mouse Galectin‑1 Monoclonal Antibody (Catalog # MAB1245) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.
Detection of Mouse Galectin‑1 by Western Blot.
Western blot shows lysates of C2C12 mouse myoblast cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Galectin‑1 Monoclonal Antibody (Catalog # MAB1245) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Galectin‑1 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Applications for Mouse Galectin‑1 Antibody
Simple Western
Sample: C2C12 mouse myoblast cell line
Western Blot
Sample: C2C12 mouse myoblast cell line
Reviewed Applications
Read 3 reviews rated 4.7 using MAB1245 in the following applications:
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Galectin-1
References
- Rabinovich, A. et al. (2002) Trends in Immunol. 23:313.
- Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741.
- Hughes, R.C. (2001) Biochimie 83:667.
- R&D Systems’ Cytokine Bulletin, Summer (2002).
- Goldring, K. et al. (2002) J. Cell Science 115:355.
Alternate Names
Gene Symbol
UniProt
Additional Galectin-1 Products
Product Documents for Mouse Galectin‑1 Antibody
Product Specific Notices for Mouse Galectin‑1 Antibody
For research use only
Related Research Areas
Citations for Mouse Galectin‑1 Antibody
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Customer Reviews for Mouse Galectin‑1 Antibody (3)
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Customer Images
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Application: Western BlotSample Tested: Cell LysatesVerified Customer | Posted 10/26/2015Works at 1:1000 <br />Specificity: Specific<br />Sensitivity: Sensitive<br />Buffer: BSA<br />Dilution: 1:1000
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Application: ImmunoprecipitationSample Tested: Cell LysatesSpecies: HumanVerified Customer | Posted 10/26/2015This antibody is very sensitive to detect galectin-1, last lane is marker, last but one is purified galectin, and the rest lanes are cell lysates from differnet cancer cell lines, so it shows that galectin-1 is actually modified.<br /> Only the last bands at bottom is galectin-1, the rest bands are IgG. <br />Specificity: Specific<br />Sensitivity: Sensitive<br />Buffer: BSA<br />Dilution: 1:1000
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Application: Immunocytochemistry/ImmunofluorescenceSample Tested: Human cellsSpecies: HumanVerified Customer | Posted 10/26/2015Specificity: Specific<br />Sensitivity: Reasonably sensitive<br />Buffer: PBS<br />Dilution: 1:250
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
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