Mouse Galectin-9 Antibody Summary
Ala2-Thr353; aa 148-178 deletion
Accession # O08573
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Galectin-9 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with Goat Anti-Mouse Galectin-9 Short Isoform Affinity-purified Polyclonal Antibody (Catalog # AF3535, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
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Galectin‑9 in Mouse Splenocytes. Galectin-9 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse Galectin-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3535) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Galectin-9
Galectin-9, also known as Ecalectin, belongs to the Galectin family (S-type lectins) of galactoside-binding proteins. It is a 36‑39 kDa protein that lacks a secretion signal sequence, but can be secreted by a non-classical pathway. Galectin-9 is a tandem-repeat Galectin with two Galectin domains joined by a linker region. Two alternative spliced variants, a short and a long isoform, exist. Galectin-9 short isoform is expressed in a variety of tissues and cells, including fibroblasts, hepatocytes, endothelial cells and astrocytes. The long isoform, however, is expressed primarily in the intestine. Galectin-9 binds TIM-3 on Th1 cells, inducing apoptosis. Mouse Galectin-9 shares 69% and 85% amino acid sequence identity with human and rat Galectin-9, respectively.
Product Datasheets
Citations for Mouse Galectin-9 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Gangliosides modulate insulin secretion by pancreatic beta cells under glucose stress
Authors: Richard Jennemann, Sylvia Kaden, Martina Volz, Viola Nordström, Silke Herzer, Roger Sandhoff et al.
Glycobiology
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N-Linked Glycosylation Regulates CD22 Organization and Function
Authors: Laabiah Wasim, Fathima Hifza Mohamed Buhari, Myuran Yoganathan, Taylor Sicard, June Ereño-Orbea, Jean-Philippe Julien et al.
Frontiers in Immunology
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Characterization of neutralizing antibodies reacting with the 213-224 amino-acid segment of human galectin-9
Authors: C Lhuillier, C Barjon, V Baloche, T Niki, A Gelin, R Mustapha, L Claër, S Hoos, Y Chiba, M Ueno, M Hirashima, M Wei, O Morales, B Raynal, N Delhem, O Dellis, P Busson
PLoS ONE, 2018-09-11;13(9):e0202512.
Species: Mouse
Sample Types: Protein
Applications: Western Blot -
Galectin-9 is Critical for Mucosal Adaptive Immunity through the TH17-IgA Axis
Authors: CC Liang, CS Li, IC Weng, HY Chen, HH Lu, CC Huang, FT Liu
Am. J. Pathol., 2018-02-17;0(0):.
Species: Mouse
Sample Types: Serum
Applications: ELISA Capture -
Astrocyte galectin-9 potentiates microglial TNF secretion.
Authors: Steelman, Andrew J, Li, Jianrong
J Neuroinflammation, 2014-08-27;11(0):144.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Galectin-9 protein is up-regulated in astrocytes by tumor necrosis factor and promotes encephalitogenic T-cell apoptosis.
Authors: Steelman A, Smith R, Welsh C, Li J
J Biol Chem, 2013-07-08;288(33):23776-87.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
TIM-3 does not act as a receptor for galectin-9.
Authors: Leitner, Judith, Rieger, Armin, Pickl, Winfried, Zlabinger, Gerhard, Grabmeier-Pfistershammer, Katharin, Steinberger, Peter
PLoS Pathog, 2013-03-21;9(3):e1003253.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Endogenous galectin-1 and acute inflammation: emerging notion of a galectin-9 pro-resolving effect.
Authors: Iqbal AJ, Sampaio AL, Maione F, Greco KV, Niki T, Hirashima M, Perretti M, Cooper D
Am. J. Pathol., 2011-03-01;178(3):1201-9.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-P, Western Blot
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Mouse alveolar macrophage- MH-S cell line. Total proteins were extracted from the cells and analysed for gal-9 expression by western blot. A lot of bands were seen and it was very difficult to determine which bands are the specific ones. According to the manual, bands boxed in a red rectangle are supposed to be the specific bands. Overall, the experiment was not at all conclusive. No bands were seen in supernatent as well. Not recommended to use for native expression detection in mouse macrophage cells.
