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Mouse Galectin‑9 Biotinylated Antibody

Catalog #: BAF3535
2 Citations Datasheet / COA / SDS
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BAF3535
Product Details
Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Galectin‑9 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Galectin-9 in Western blots. In this format, less than 1% cross-reactivity with recombinant human (rh) Galectin‑2, rhGalectin‑8, recombinant mouse (rm) Galectin-1, rmGalectin-3, rmGalectin-4, and rmGalectin-7 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Galectin‑9 short isoform
Ala2-Thr353; aa148-178 deleted
Accession # O08573
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Galectin‑9 Short Isoform

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Galectin-9

Galectin-9, also known as Ecalectin, belongs to the Galectin family (S-type lectins) of galactoside-binding proteins. It is a 36 - 39 kDa protein that lacks a secretion signal sequence, but can be secreted by a non-classical pathway. Galectin-9 is a tandem-repeat Galectin with two Galectin domains joined by a linker region. Two alternative spliced variants, a short and a long isoform, exist. Galectin-9 short isoform is expressed in a variety of tissues and cells, including fibroblasts, hepatocytes, endothelial cells and astrocytes. The long isoform, however, is expressed primarily in the intestine. Galectin-9 binds TIM-3 on Th1 cells, inducing apoptosis. Mouse Galectin-9 shares 69% and 85% amino acid sequence identity with human and rat Galectin-9, respectively.

Entrez Gene IDs
3965 (Human); 16859 (Mouse)
Alternate Names
Ecalectin; GAL9; gal-9; galectin 9; Galectin9; Galectin-9; HUAT; lectin, galactoside-binding, soluble, 9; LGALS9; LGALS9A; MGC117375; MGC125973; MGC125974; Tumor antigen HOM-HD-21; urate transporter/channel protein

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Citations for Mouse Galectin‑9 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Galectin-9 is Critical for Mucosal Adaptive Immunity through the TH17-IgA Axis
    Authors: CC Liang, CS Li, IC Weng, HY Chen, HH Lu, CC Huang, FT Liu
    Am. J. Pathol., 2018-02-17;0(0):.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Detection
  2. Influence of galectin-9/Tim-3 interaction on herpes simplex virus-1 latency.
    Authors: Reddy PB, Sehrawat S, Suryawanshi A
    J. Immunol., 2011-10-21;187(11):5745-55.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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