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Mouse GLI-2 Antibody

Catalog #: AF3635
44 Citations 3 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3635
AF3635-SP
GLI‑2 in Mouse Embryo.
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Product Details
Citations (44)
FAQs
Supplemental Products
Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse GLI-2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse GLI-2 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse GLI-2
Met1-Glu416
Accession # NP_001074594
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse GLI‑2
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry GLI-2 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

GLI‑2 in Mouse Embryo. GLI-2 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse GLI-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3635) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to developing muscle. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GLI-2

Mouse GLI-2 is a 185 kDa biopotential transcription regulator of the hedgehog signaling pathway. It contains 5 conserved tandem C2H2 zinc finger domains that are flanked by a repression domain at the N-terminal region and an activation domain in the C-terminal region. At least two additional isoforms that have amino acid substitutions/truncations in the region corresponding to amino acid residues 192‑350 of isoform 1 have been described. Transcriptional activity of GLI-2 is regulated by proteolytic processing and degradation. Within the region used as immunogen, mouse GLI-2 shares 91% and 97% amino acid sequence homology with that of human and rat GLI-2, respectively.

Long Name
GLI-Kruppel family member GLI2
Entrez Gene IDs
2736 (Human); 14633 (Mouse); 304729 (Rat)
Alternate Names
GLI family zinc finger 2; GLI2; GLI-2; GLI-Kruppel family member GLI2; glioma-associated oncogene family zinc finger 2; HPE9tax helper protein 1; oncogene GLI2; tax helper protein 2; Tax helper protein; tax-responsive element-2 holding protein; tax-responsive element-25-bp sequence binding protein; THP; THP1; THP2; zinc finger protein GLI2

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Citations for Mouse GLI-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

44 Citations: Showing 1 - 10
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  1. Activation of Hedgehog signaling by loss of GNAS causes heterotopic ossification.
    Authors: Regard JB, Malhotra D, Gvozdenovic-Jeremic J et al.
    Nat Med.
  2. Developmental phosphoproteomics identifies the kinase CK2 as a driver of Hedgehog signaling and a therapeutic target in medulloblastoma
    Authors: Teresa Purzner, James Purzner, Taylor Buckstaff, Giorgio Cozza, Sharareh Gholamin, Jessica M. Rusert et al.
    Science Signaling
  3. Myomegalin regulates Hedgehog pathway by controlling PDE4D at the centrosome
    Authors: Hualing Peng, Jingyi Zhang, Amanda Ya, Winston Ma, Sammy Villa, Shahar Sukenik et al.
    Molecular Biology of the Cell
  4. Ankmy2 Prevents Smoothened-Independent Hyperactivation of the Hedgehog Pathway via Cilia-Regulated Adenylyl Cyclase Signaling
    Authors: Bandarigoda Nipunika Somatilaka, Sun-Hee Hwang, Vivek Reddy Palicharla, Kevin Andrew White, Hemant Badgandi, John Michael Shelton et al.
    Developmental Cell
  5. Phosphorylation of Ci/Gli by Fused Family Kinases Promotes Hedgehog Signaling
    Authors: Yuhong Han, Bing Wang, Yong Suk Cho, Jian Zhu, Jiang Wu, Yongbin Chen et al.
    Developmental Cell
  6. Differential requirement of SUFU in tissue development discovered in a hypomorphic mouse model
    Authors: Maria A. Hoelzl, Karin Heby-Henricson, Marco Gerling, José M. Dias, Raoul V. Kuiper, Cornelius Trünkle et al.
    Developmental Biology
  7. The novel ciliogenesis regulator DYRK2 governs Hedgehog signaling during mouse embryogenesis
    Authors: Saishu Yoshida, Katsuhiko Aoki, Ken Fujiwara, Takashi Nakakura, Akira Kawamura, Kohji Yamada et al.
    eLife
  8. Ciliary and extraciliary Gpr161 pools repress hedgehog signaling in a tissue-specific manner
    Authors: Sun-Hee Hwang, Bandarigoda N Somatilaka, Kevin White, Saikat Mukhopadhyay
    eLife
  9. Multiple Shh signaling centers participate in fungiform papilla and taste bud formation and maintenance.
    Authors: Liu HX, Ermilov A, Grachtchouk M et al.
    Dev Biol
  10. Epigenetically regulated PAX6 drives cancer cells toward a stem-like state via GLI-SOX2 signaling axis in lung adenocarcinoma
    Authors: A Ooki, W Dinalankar, L Marchionni, JJ Tsay, C Goparaju, Z Maleki, WN Rom, HI Pass, MO Hoque
    Oncogene, 2018-07-06;0(0):.
  11. ERAP1 promotes Hedgehog-dependent tumorigenesis by controlling USP47-mediated degradation of beta TrCP
    Authors: Francesca Bufalieri, Paola Infante, Flavia Bernardi, Miriam Caimano, Paolo Romania, Marta Moretti et al.
    Nature Communications
  12. Maml1 acts cooperatively with Gli proteins to regulate sonic hedgehog signaling pathway
    Authors: Roberta Quaranta, Maria Pelullo, Sabrina Zema, Francesca Nardozza, Saula Checquolo, Dieter Matthias Lauer et al.
    Cell Death & Disease
  13. Targeted protein degradation reveals BET bromodomains as the cellular target of Hedgehog pathway inhibitor-1
    Authors: Meropi Bagka, Hyeonyi Choi, Margaux Héritier, Hanna Schwaemmle, Quentin T. L. Pasquer, Simon M. G. Braun et al.
    Nature Communications
  14. Bicyclic Imidazolium Inhibitors of Gli Transcription Factor Activity
    Authors: Marisa E. Hom, Alison E. Ondrus, Tomoyo Sakata‐Kato, Paul G. Rack, James K. Chen
    ChemMedChem
  15. An ancient yet flexible cis-regulatory architecture allows localized Hedgehog tuning by patched/Ptch1
    Authors: David S Lorberbaum, Andrea I Ramos, Kevin A Peterson, Brandon S Carpenter, David S Parker, Sandip De et al.
    eLife
  16. Protein kinase inhibitor SU6668 attenuates positive regulation of Gli proteins in cancer and multipotent progenitor cells
    Authors: Alla Piirsoo, Lagle Kasak, Mari-Liis Kauts, Mart Loog, Kairit Tints, Piia Uusen et al.
    Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
  17. Chronic liver injury alters driver mutation profiles in hepatocellular carcinoma in mice
    Authors: Jesse D. Riordan, Charlotte R. Feddersen, Barbara R. Tschida, Pauline J. Beckmann, Vincent W. Keng, Michael A. Linden et al.
    Hepatology
  18. The Proteasome and Cul3-Dependent Protein Ubiquitination Is Required for Gli Protein-Mediated Activation of Gene Expression in the Hedgehog Pathway
    Authors: U?pie?ski, T;Niewiadomski, P;
    Cells
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. PP2A phosphatase regulatory subunit PPP2R3C is a new positive regulator of the hedgehog signaling pathway
    Authors: Baran, B;Derua, R;Janssens, V;Niewiadomski, P;
    Cellular signalling
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  20. Increasing Ciliary ARL13B Expression Drives Active and Inhibitor-Resistant Smoothened and GLI into Glioma Primary Cilia
    Authors: Shi, P;Tian, J;Mallinger, JC;Ling, D;Deleyrolle, LP;McIntyre, JC;Caspary, T;Breunig, JJ;Sarkisian, MR;
    Cells
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  21. Zic5 stabilizes Gli3 via a non-transcriptional mechanism during retinal development
    Authors: J Sun, J Yoon, M Lee, HK Lee, YS Hwang, IO Daar
    Cell Reports, 2022-02-01;38(5):110312.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  22. Activation of Cilia-Independent Hedgehog/GLI1 Signaling as a Novel Concept for Neuroblastoma Therapy
    Authors: A Koeniger, A Brichkina, I Nee, L Dempwolff, A Hupfer, I Galperin, F Finkernage, A Nist, T Stiewe, T Adhikary, W Diederich, M Lauth
    Cancers, 2021-04-15;13(8):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  23. A Shh/Gli-driven three-node timer motif controls temporal identity and fate of neural stem cells
    Authors: JM Dias, Z Alekseenko, A Jeggari, M Boareto, J Vollmer, M Kozhevniko, H Wang, MP Matise, A Alexeyenko, D Iber, J Ericson
    Science Advances, 2020-09-16;6(38):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Immunoprecipitation, Western Blot
  24. A Smo/Gli Multitarget Hedgehog Pathway Inhibitor Impairs Tumor Growth
    Authors: L Lospinoso, D Quaglio, I Basili, F Ghirga, F Bufalieri, M Caimano, S Balducci, M Moretti, I Romeo, E Loricchio, M Maroder, B Botta, M Mori, P Infante, L Di Marcotu
    Cancers (Basel), 2019-10-09;11(10):.
    Species: Mouse
    Sample Types: Tissue Lysates
    Applications: Western Blot
  25. NRF2-dependent gene expression promotes ciliogenesis and Hedgehog signaling
    Authors: A Martin-Hur, R Martin-Mor, N Robledinos, R Blanco, I Palacios-B, I Lastres-Be, A Cuadrado, FR Garcia-Gon
    Sci Rep, 2019-09-25;9(1):13896.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  26. Fine-Tuning of Shh/Gli Signaling Gradient by Non-proteolytic Ubiquitination during Neural Patterning
    Authors: P Ma, NN Song, Y Li, Q Zhang, L Zhang, L Zhang, Q Kong, L Ma, X Yang, B Ren, C Li, X Zhao, Y Li, Y Xu, X Gao, YQ Ding, B Mao
    Cell Rep, 2019-07-09;28(2):541-553.e4.
    Species: Human, Mouse
    Sample Types: Cell Fractions, Cell Lysates
    Applications: Western Blot
  27. Itch/?-arrestin2-dependent non-proteolytic ubiquitylation of SuFu controls Hedgehog signalling and medulloblastoma tumorigenesis
    Authors: P Infante, R Faedda, F Bernardi, F Bufalieri, L Lospinoso, R Alfonsi, D Mazzà, M Siler, S Coni, A Po, M Petroni, E Ferretti, M Mori, E De Smaele, G Canettieri, C Capalbo, M Maroder, I Screpanti, M Kool, SM Pfister, D Guardavacc, A Gulino, L Di Marcotu
    Nat Commun, 2018-03-07;9(1):976.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  28. TSC1 and TSC2 regulate cilia length and canonical Hedgehog signaling via different mechanisms
    Authors: T Rosengren, LJ Larsen, LB Pedersen, ST Christense, LB Møller
    Cell. Mol. Life Sci., 2018-02-02;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  29. EMT programs promote basal mammary stem cell and tumor-initiating cell stemness by inducing primary ciliogenesis and Hedgehog signaling
    Authors: VJ Guen, TE Chavarria, C Kröger, X Ye, RA Weinberg, JA Lees
    Proc. Natl. Acad. Sci. U.S.A., 2017-11-20;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  30. Suppressor of Fused Restraint of Hedgehog Activity Level is Critical for Osteogenic Proliferation and Differentiation during Calvarial Bone Development
    Authors: J Li, Y Cui, J Xu, Q Wang, X Yang, Y Li, X Zhang, M Qiu, Z Zhang, Z Zhang
    J. Biol. Chem., 2017-08-09;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  31. Regulation of Gli ciliary localization and Hedgehog signaling by the PY-NLS/karyopherin-?2 nuclear import system
    Authors: Y Han, Y Xiong, X Shi, J Wu, Y Zhao, J Jiang
    PLoS Biol., 2017-08-04;15(8):e2002063.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  32. Spop regulates Gli3 activity and Shh signaling in dorsoventral patterning of the mouse spinal cord
    Authors: H Cai, A Liu
    Dev. Biol., 2017-04-12;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  33. Spop promotes skeletal development and homeostasis by positively regulating Ihh signaling
    Proc. Natl. Acad. Sci. U.S.A, 2016-12-05;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  34. Ciliary Entry of the Hedgehog Transcriptional Activator Gli2 Is Mediated by the Nuclear Import Machinery but Differs from Nuclear Transport in Being Imp-?/?1-Independent
    PLoS ONE, 2016-08-31;11(8):e0162033.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  35. Pharmacological GLI2 inhibition prevents myofibroblast cell-cycle progression and reduces kidney fibrosis.
    Authors: Kramann R, Fleig S, Schneider R, Fabian S, DiRocco D, Maarouf O, Wongboonsin J, Ikeda Y, Heckl D, Chang S, Rennke H, Waikar S, Humphreys B
    J Clin Invest, 2015-07-20;125(8):2935-51.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  36. Inversion of Sonic hedgehog action on its canonical pathway by electrical activity.
    Authors: Belgacem Y, Borodinsky L
    Proc Natl Acad Sci U S A, 2015-03-17;112(13):4140-5.
    Species: Xenopus
    Sample Types: Embryo
    Applications: IHC-P, Western Blot
  37. Ciliopathy proteins regulate paracrine signaling by modulating proteasomal degradation of mediators.
    Authors: Liu Y, Tsai I, Morleo M, Oh E, Leitch C, Massa F, Lee B, Parker D, Finley D, Zaghloul N, Franco B, Katsanis N
    J Clin Invest, 2014-04-01;124(5):2059-70.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  38. The output of Hedgehog signaling is controlled by the dynamic association between Suppressor of Fused and the Gli proteins.
    Authors: Humke EW, Dorn KV, Milenkovic L, Scott MP, Rohatgi R
    Genes Dev., 2010-04-01;24(7):670-82.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  39. Long non-coding RNA HOTAIR induces GLI2 expression through Notch signalling in systemic sclerosis dermal fibroblasts
    Authors: CW Wasson, RL Ross, R Wells, C Corinaldes, IC Georgiou, NA Riobo-Del, F Del Galdo
    Arthritis Research & Therapy, 2020-12-10;22(1):286.
  40. Genetic deletion of genes in the cerebellar rhombic lip lineage can stimulate compensation through adaptive reprogramming of ventricular zone-derived progenitors
    Authors: Alexandre Wojcinski, Morgane Morabito, Andrew K. Lawton, Daniel N. Stephen, Alexandra L. Joyner
    Neural Development
  41. HEDGEHOG/GLI-E2F1 axis modulates iASPP expression and function and regulates melanoma cell growth
    Authors: S Pandolfi, V Montagnani, A Lapucci, B Stecca
    Cell Death & Differentiation
  42. Gastritis promotes an activated bone marrow-derived mesenchymal stem cell with a phenotype reminiscent of a cancer-promoting cell.
    Authors: Donnelly J, Engevik A, Engevik M, Schumacher M, Xiao C, Yang L, Worrell R, Zavros Y
    Dig Dis Sci, 2013-11-08;59(3):569-82.
  43. A Druggable UHRF1/DNMT1/GLI Complex Regulates Sonic Hedgehog–Dependent Tumor Growth
    Authors: Fan Yang, Jezabel Rodriguez-Blanco, Jun Long, Marzena Swiderska-Syn, Daniel T. Wynn, Bin Li et al.
    Molecular Cancer Research
  44. The ciliary phosphatidylinositol phosphatase Inpp5e plays positive and negative regulatory roles in Shh signaling
    Authors: Sandii Constable, Alyssa B. Long, Katharine A. Floyd, Stéphane Schurmans, Tamara Caspary
    Development

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Reviews for Mouse GLI-2 Antibody

Average Rating: 3 (Based on 3 Reviews)

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Mouse GLI-2 Antibody
By Oliver Tkaczyk on 08/02/2020
Application: WB Sample Tested: NIH-3T3 mouse embryonic fibroblast cell line Species: Mouse
Western Blot Mouse GLI-2 Antibody AF3635
Mouse GLI-2 Antibody
By Brygida Baran on 07/22/2020
Application: IP Sample Tested: NIH-3T3 mouse embryonic fibroblast cell line Species: Mouse
Immunoprecipitation Mouse GLI-2 Antibody AF3635
Mouse GLI-2 Antibody
By Anonymous on 07/08/2019
Application: WB Sample Tested: MEF (fibroblast) Species: Mouse

This antibody recognizes many bands, and the major expected bands are not stronger (or maybe much weaker) than those non-specific bands.
***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***

Western Blot Mouse GLI-2 Antibody AF3635