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Mouse HGFR/c-MET Antibody

Catalog #: AF527
35 Citations 1 Review Datasheet / COA / SDS
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AF527
AF527-SP
HGF R/c‑MET in HT‑29 and U937 Human Cell Lines.
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Citations (35)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse HGFR/c-MET Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse HGF R/c-MET in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) HGF R and less than 1% cross-reactivity with rhMSP R is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse HGF R/c-MET
Glu25-Asn929
Accession # P16056
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse HGF R/c-MET Fc Chimera (Catalog # 527-ME)
Immunohistochemistry
5-15 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.3-1 µg/mL of this antibody will block 50% of the binding of 5 ng/mL of Recombinant Human HGF (Catalog # 256-GF) to immobilized Recombinant Mouse HGF R/c-MET Fc Chimera (Catalog # 527‑ME) coated at 1 µg/mL (100 µL/well). At 20 μg/mL, this antibody will block >90% of the binding.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry HGF R/c-MET antibody in HT-29 and U937 Human Cell Lines by Immunocytochemistry (ICC). View Larger

HGF R/c‑MET in HT‑29 and U937 Human Cell Lines. HGF R/c-MET was detected in immersion fixed HT-29 human colon adenocarcinoma cell line (positive control, left panel) and U937 human histiocytic lymphoma cell line (negative control, right panel) using Goat Anti-Mouse HGF R/c-MET Antigen Affinity-purified Polyclonal Antibody (Catalog # AF527) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry HGF R/c-MET antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

HGF R/c‑MET in Mouse Embryo. HGF R/c-MET was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse HGF R/c-MET Antigen Affinity-purified Polyclonal Antibody (Catalog # AF527) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HGFR/c-MET

HGF R, also known as Met (from N-methyl-N’-nitro-N-nitrosoguanidine induced), is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGF R is synthesized as a single chain precursor which undergoes cotranslational proteolytic cleavage. This generates a mature HGF R that is a disulfide-linked dimer composed of a 50 kDa extracellular  alpha chain and a 145 kDa transmembrane beta chain (1, 2). The extracellular domain (ECD) contains a seven bladed beta -propeller sema domain, a cysteine-rich PSI/MRS, and four Ig-like E-set domains, while the cytoplasmic region includes the tyrosine kinase domain (3, 4). An alternately spliced form of mouse HGF R lacks a cytoplasmic juxtamembrane region important for regulation of signal transduction (5, 6). The sema domain, which is formed by both the alpha and beta chains of HGF R, mediates both ligand binding and receptor dimerization (3, 7). Ligand-induced tyrosine phosphorylation in the cytoplasmic region activates the kinase domain and provides docking sites for multiple SH2-containing molecules (8, 9). HGF stimulation induces HGF R downregulation via internalization and proteasome-dependent degradation (10). In the absence of ligand, HGF R forms non-covalent complexes with a variety of membrane proteins including CD44v6, CD151, EGF R, Fas, integrin  alpha 6/ beta 4, plexins B1, 2, 3, and MSP R/Ron (11-18). Ligation of one complex component triggers activation of the other, followed by cooperative signaling effects (11-18). Formation of some of these heteromeric complexes is a requirement for epithelial cell morphogenesis and tumor cell invasion (11, 15, 16). Paracrine induction of epithelial cell scattering and branching tubulogenesis results from the stimulation of HGF R on undifferentiated epithelium by HGF released from neighboring mesenchymal cells (19). Genetic polymorphisms, chromosomal translocation,
over-expression, and additional splicing and proteolytic cleavage of HGF R have been described in a wide range of cancers (1). Within the ECD, mouse HGF R shares 87%, 87%, and 94% amino acid sequence identity with canine, human, and rat HGF R, respectively.

References
  1. Birchmeier, C. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:915.
  2. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
  3. Gherardi, E. et al. (2003) Proc. Natl. Acad. Sci. USA 100:12039.
  4. Chan, A.M. et al. (1988) Oncogene 2:593.
  5. Lee, C.-C. and K.M. Yamada (1994) J. Biol. Chem. 269:19457.
  6. Lee, C.-C., et al. (1995) J. Biol. Chem. 270:507.
  7. Kong-Beltran, M. et al. (2004) Cancer Cell 6:75.
  8. Naldini, L. et al. (1991) Mol. Cell. Biol. 11:1793.
  9. Ponzetto, C. et al. (1994) Cell 77:261.
  10. Jeffers, M. et al. (1997) Mol. Cell. Biol. 17:799.
  11. Orian-Rousseau, V. et al. (2002) Genes Dev. 16:3074.
  12. Klosek, S.K. et al. (2005) Biochem. Biophys. Res. Commun. 336:408.
  13. Jo, M. et al. (2000) J. Biol. Chem. 275:8806.
  14. Wang, X. et al. (2002) Mol. Cell 9:411.
  15. Trusolino, L. et al. (2001) Cell 107:643.
  16. Giordano, S. et al. (2002) Nat. Cell Biol. 4:720.
  17. Conrotto, P. et al. (2004) Oncogene 23:5131.
  18. Follenzi, A. et al. (2000) Oncogene 19:3041.
  19. Sonnenberg, E. et al. (1993) J. Cell Biol. 123:223.
Long Name
Hepatocyte Growth Factor Receptor
Entrez Gene IDs
4233 (Human); 17295 (Mouse)
Alternate Names
AUTS9; cMET; c-MET; EC 2.7.10; EC 2.7.10.1; hepatocyte growth factor receptor; HGF R; HGF receptor; HGF/SF receptor; HGFR; Met (c-Met); met proto-oncogene (hepatocyte growth factor receptor); met proto-oncogene tyrosine kinase; MET; oncogene MET; Proto-oncogene c-Met; RCCP2; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met

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Citations for Mouse HGFR/c-MET Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

35 Citations: Showing 1 - 10
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  1. Hepatocyte Growth Factor and MET Support Mouse Enteric Nervous System Development, the Peristaltic Response, and Intestinal Epithelial Proliferation in Response to Injury
    Authors: Marina Avetisyan, Hongtao Wang, Ellen Merrick Schill, Saya Bery, John R. Grider, John A. Hassell et al.
    The Journal of Neuroscience
  2. Specific Connectivity and Unique Molecular Identity of MET Receptor Tyrosine Kinase Expressing Serotonergic Neurons in the Caudal Dorsal Raphe Nuclei
    Authors: Ryan J. Kast, Hsiao-Huei Wu, Piper Williams, Patricia Gaspar, Pat Levitt
    ACS Chemical Neuroscience
  3. Prenatal Expression of MET Receptor Tyrosine Kinase in the Fetal Mouse Dorsal Raphe Nuclei and the Visceral Motor/Sensory Brainstem
    Authors: Hsiao-Huei Wu, Pat Levitt
    Developmental Neuroscience
  4. SOX9-positive pituitary stem cells differ according to their position in the gland and maintenance of their progeny depends on context
    Authors: Rizzoti, K;Chakravarty, P;Sheridan, D;Lovell-Badge, R;
    Science advances
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Novel rAAV vector mediated intrathecal HGF delivery has an impact on neuroimmune modulation in the ALS motor cortex with TDP-43 pathology
    Authors: B Genç, B Nho, H Seung, B Helmold, H Park, Ö Gözütok, S Kim, J Park, S Ye, H Lee, N Lee, SS Yu, S Kim, J Lee, H Özdinler
    Gene Therapy, 2023-02-24;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Fibroblast-derived Hgf controls recruitment and expansion of muscle during morphogenesis of the mammalian diaphragm
    Authors: EM Sefton, M Gallardo, CE Tobin, BC Collins, MP Colasanto, AJ Merrell, G Kardon
    Elife, 2022-09-26;11(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. The study of direct and indirect effects of radiofrequency ablation on tumor microenvironment in liver tumor animal model
    Authors: AN Jiang, B Wang, S Wang, K Zhao, H Wu, K Yan, W Wu, W Yang
    BMC Cancer, 2022-06-17;22(1):663.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Two-Chains Tissue Plasminogen Activator Unifies Met and NMDA Receptor Signalling to Control Neuronal Survival
    Authors: E Hedou, S Douceau, A Chevilley, A Varangot, AM Thiebaut, H Triniac, I Bardou, C Ali, M Maillasson, T Crepaldi, P Comoglio, E Lemarchand, V Agin, BD Roussel, D Vivien
    International Journal of Molecular Sciences, 2021-12-15;22(24):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  9. Wnt signaling enhances macrophage responses to IL-4 and promotes resolution of atherosclerosis
    Authors: A Weinstock, K Rahman, O Yaacov, H Nishi, P Menon, CA Nikain, ML Garabedian, S Pena, N Akbar, BE Sansbury, SP Heffron, J Liu, G Marecki, D Fernandez, EJ Brown, KV Ruggles, SA Ramsey, C Giannarell, M Spite, RP Choudhury, P Loke, EA Fisher
    Elife, 2021-03-15;10(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Aberrant activation of hepatocyte growth factor/MET signaling promotes beta-catenin-mediated prostatic tumorigenesis
    Authors: J Aldahl, J Mi, A Pineda, WK Kim, A Olson, E Hooker, Y He, EJ Yu, V Le, DH Lee, J Geradts, Z Sun
    J. Biol. Chem., 2019-12-09;295(2):631-644.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Activation of hepatocyte growth factor/MET signaling initiates oncogenic transformation and enhances tumoraggressiveness in the murine prostate
    Authors: J Mi, E Hooker, S Balog, H Zeng, DT Johnson, Y He, EJ Yu, H Wu, V Le, DH Lee, J Aldahl, ML Gonzalgo, Z Sun
    J. Biol. Chem., 2018-11-06;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  12. Pulmonary pericytes regulate lung morphogenesis
    Authors: K Kato, R Diéguez-Hu, DY Park, SP Hong, S Kato-Azuma, S Adams, M Stehling, B Trappmann, JL Wrana, GY Koh, RH Adams
    Nat Commun, 2018-06-22;9(1):2448.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  13. Hepatocyte Growth Factor-c-MET Signaling Mediates the Development of Nonsensory Structures of the Mammalian Cochlea and Hearing
    J Neurosci, 2016-08-03;36(31):8200-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  14. Beta 1-integrin-c-Met cooperation reveals an inside-in survival signalling on autophagy-related endomembranes
    Authors: Rachel Barrow-McG
    Nat Commun, 2016-06-23;7(0):11942.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. Receptor Tyrosine Kinase Met Promotes Cell Survival via Kinase-Independent Maintenance of Integrin ?3?1
    Authors: Lia Tesfay
    Mol Biol Cell, 2016-06-15;0(0):.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: IHC-Fr, Western Blot
  16. MET is required for the recruitment of anti-tumoural neutrophils.
    Authors: Finisguerra V, Di Conza G, Di Matteo M, Serneels J, Costa S, Thompson A, Wauters E, Walmsley S, Prenen H, Granot Z, Casazza A, Mazzone M
    Nature, 2015-05-18;522(7556):349-53.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ELISA Development (Capture)
  17. Regulation of hepatocyte growth factor in mice with pneumonia by peptidases and trans-alveolar flux.
    Authors: Raymond W, Xu X, Nimishakavi S, Le C, McDonald D, Caughey G
    PLoS ONE, 2015-05-04;10(5):e0125797.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. Targeting matriptase in breast cancer abrogates tumour progression via impairment of stromal-epithelial growth factor signalling.
    Authors: Zoratti G, Tanabe L, Varela F, Murray A, Bergum C, Colombo E, Lang J, Molinolo A, Leduc R, Marsault E, Boerner J, List K
    Nat Commun, 2015-04-15;6(0):6776.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  19. Recombinant insulin-like growth factor-1 activates satellite cells in the mouse urethral rhabdosphincter.
    Authors: Wei W, Howard P, Macarak E
    BMC Urol, 2013-11-26;13(0):62.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  20. c-Met and its ligand hepatocyte growth factor/scatter factor regulate mature B cell survival in a pathway induced by CD74.
    Authors: Gordin M, Tesio M, Cohen S
    J. Immunol., 2010-07-16;185(4):2020-31.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Western Blot
  21. Enhanced c-Met activity promotes G-CSF-induced mobilization of hematopoietic progenitor cells via ROS signaling.
    Authors: Tesio M, Golan K, Corso S
    Blood, 2010-06-28;117(2):419-28.
    Species: Mouse
    Sample Types: Cell Lysates, In Vivo, Whole Cells
    Applications: Flow Cytometry, Neutralization, Western Blot
  22. Expression of the HGF receptor c-met by macrophages in experimental autoimmune encephalomyelitis.
    Authors: Moransard M, Sawitzky M, Fontana A, Suter T
    Glia, 2010-04-01;58(5):559-71.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-Fr, Immunoprecipitation, Western Blot
  23. High concentrations of HGF inhibit skeletal muscle satellite cell proliferation in vitro by inducing expression of myostatin: a possible mechanism for reestablishing satellite cell quiescence in vivo.
    Authors: Yamada M, Tatsumi R, Yamanouchi K, Hosoyama T, Shiratsuchi S, Sato A, Mizunoya W, Ikeuchi Y, Furuse M, Allen RE
    Am. J. Physiol., Cell Physiol., 2009-12-09;298(3):C465-76.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  24. Possible implication of satellite cells in regenerative motoneuritogenesis: HGF upregulates neural chemorepellent Sema3A during myogenic differentiation.
    Authors: Tatsumi R, Sankoda Y, Anderson JE, Sato Y, Mizunoya W, Shimizu N, Suzuki T, Yamada M, Rhoads RP, Ikeuchi Y, Allen RE
    Am. J. Physiol., Cell Physiol., 2009-06-10;297(2):C238-52.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  25. A role for calcium-calmodulin in regulating nitric oxide production during skeletal muscle satellite cell activation.
    Authors: Tatsumi R, Wuollet AL, Tabata K, Nishimura S, Tabata S, Mizunoya W, Ikeuchi Y, Allen RE
    Am. J. Physiol., Cell Physiol., 2009-01-21;296(4):C922-9.
    Species: Rat
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  26. Purification and characterization of mouse lacrimal gland epithelial cells and reconstruction of an acinarlike structure in three-dimensional culture.
    Authors: Ueda Y, Karasawa Y, Satoh Y, Nishikawa S, Imaki J, Ito M
    Invest. Ophthalmol. Vis. Sci., 2008-12-20;50(5):1978-87.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  27. Ets-1 triggers and orchestrates the malignant phenotype of mammary cancer cells within their matrix environment.
    Authors: Furlan A, Vercamer C, Desbiens X, Pourtier A
    J. Cell. Physiol., 2008-06-01;215(3):782-93.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  28. A selective small molecule inhibitor of c-Met, PHA-665752, reverses lung premalignancy induced by mutant K-ras.
    Authors: Yang Y, Wislez M, Fujimoto N, Prudkin L, Izzo JG, Uno F, Ji L, Hanna AE, Langley RR, Liu D, Johnson FM, Wistuba I, Kurie JM
    Mol. Cancer Ther., 2008-04-01;7(4):952-60.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  29. Gastrointestinal hormones cause rapid c-Met receptor down-regulation by a novel mechanism involving clathrin-mediated endocytosis and a lysosome-dependent mechanism.
    Authors: Hoffmann KM, Tapia JA, Berna MJ, Thill M, Braunschweig T, Mantey SA, Moody TW, Jensen RT
    J. Biol. Chem., 2006-10-10;281(49):37705-19.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  30. A recombinant single-chain IL-7/HGFbeta hybrid cytokine induces juxtacrine interactions of the IL-7 and HGF (c-Met) receptors and stimulates the proliferation of CFU-S12, CLPs, and pre-pro-B cells.
    Authors: Lai L, Zeff RA, Goldschneider I
    Blood, 2005-11-22;107(5):1776-84.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Neutralization, Western Blot
  31. Distinct projection targets define subpopulations of mouse brainstem vagal neurons that express the autism-associated MET receptor tyrosine kinase
    Authors: Anna Kamitakahara, Hsiao-Huei Wu, Pat Levitt
    Journal of Comparative Neurology
  32. cMET inhibitor crizotinib impairs angiogenesis and reduces tumor burden in the C3(1)-Tag model of basal-like breast cancer
    Authors: Alyssa J. Cozzo, Sneha Sundaram, Ottavia Zattra, Yuanyuan Qin, Alex J. Freemerman, Luma Essaid et al.
    SpringerPlus
  33. MET Receptor Tyrosine Kinase Regulates Lifespan Ultrasonic Vocalization and Vagal Motor Neuron Development
    Authors: Anna K. Kamitakahara, Ramin Ali Marandi Ghoddousi, Alexandra L. Lanjewar, Valerie M. Magalong, Hsiao-Huei Wu, Pat Levitt
    Frontiers in Neuroscience
  34. Failure to ubiquitinate c-Met leads to hyperactivation of mTOR signaling in a mouse model of autosomal dominant polycystic kidney disease
    Authors: Shan Qin, Mary Taglienti, Surya M. Nauli, Leah Contrino, Ayumi Takakura, Jing Zhou et al.
    Journal of Clinical Investigation
  35. Tumour growth stimulation following partial hepatectomy in mice is associated with increased upregulation of c-Met
    Authors: Nadia Harun, Patricia Costa, C. Christophi
    Clinical & Experimental Metastasis

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Mouse HGFR/c-MET Antibody
By Anonymous on 01/18/2022
Application: IHC Sample Tested: Colon cancer tissue Species: Mouse
Immunohistochemistry Mouse HGFR/c-MET Antibody AF527