Mouse IgE Antibody

Catalog #: MAB9935 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9935-100

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Mouse IgE Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IgE in Direct Elisa and Western blots.

Source

Monoclonal Rat IgG_2A Clone # 343527

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse IgE

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse IgE by Western Blot. Western blot shows lysates of purified Mouse IgM (negative control), purified Mouse IgA (negative control), purified Mouse IgG(negative control), and human purified Mouse IgE. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse IgE Monoclonal Antibody (Catalog # MAB9935) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IgE at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgE

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin E

Alternate Names

IgE; immunoglobulin heavy constant epsilon