Mouse IGFBP-2 Antibody Summary
Glu35-Gln305
Accession # CAA57270
Applications
Measured by its ability to neutralize IGFBP‑2 inhibition of IGF‑II-dependent proliferation in the MCF‑7 human breast cancer cell line. At 30 μg/mL, this antibody will neutralize approximately 60 - 80% of the activity due to 0.5 μg/mL rmIGFBP-2 in the presence of 30 ng/mL of rmIGFII.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IGFBP-2
The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBPs, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF.
Mouse IGFBP-2 cDNA encodes a 305 amino acid (aa) residue precursor protein with a putative 34 aa residue signal peptide that is processed to generate the 271 aa residue mature protein. Mouse and human IGFBP-2 share approximately 82% homology. IGFBP-2 contains an integrin receptor recognition sequence (RGD sequence) but lacks potential N-linked glycosylation sites. During development, IGFBP-2 is expressed in a number of tissues. The highest expression level is found in the central nervous system. In adults, high expression levels are also detected in the central nervous system and in a number of reproductive tissues. IGFBP-2 binds preferentially to IGF-II, exhibiting a 2-10 fold higher affinity for IGF-II than for IGF-I.
- Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3.
- Kelley, K.M. et al. (1996) Int. J. Biochem. Cell Biol. 28:619.
- Schuller, A.G.P. et al. (1994) Mol. Cell. Endoc. 104:57.
- Landwehr, J. et al. (1993) Gene 124:281.
Product Datasheets
Citations for Mouse IGFBP-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Age-related increases in IGFBP2 increase melanoma cell invasion and lipid synthesis
Authors: Alicea, GM;Portuallo, ME;Patel, P;Fane, ME;Carey, AE;Speicher, D;Tang, HY;Kossenkov, AV;Rebecca, VW;Wirtz, DG;Weeraratna, AT;
bioRxiv : the preprint server for biology
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Aberrant astrocyte protein secretion contributes to altered neuronal development in multiple models of neurodevelopmental disorders
Authors: ALM Caldwell, L Sancho, J Deng, A Bosworth, A Miglietta, JK Diedrich, MN Shokhirev, NJ Allen
Nature Neuroscience, 2022-08-30;25(9):1163-1178.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Spatial distribution of insulin-like growth factor binding protein-2 following hypoxic-ischemic injury.
Authors: Fletcher L, Isgor E, Sprague S, Williams L, Alajajian B, Jimenez D, Digicaylioglu M
BMC Neurosci, 2013-12-21;14(0):158.
Species: Mouse, Rat
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC -
Decreased expression of extracellular matrix proteins and trophic factors in the amygdala complex of depressed mice after chronic immobilization stress.
BMC Neurosci, 2012-06-06;13(0):58.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Bi-compartmental communication contributes to the opposite proliferative behavior of Notch1-deficient hair follicle and epidermal keratinocytes.
Authors: Lee J, Basak JM, Demehri S, Kopan R
Development, 2007-07-04;134(15):2795-806.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
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