Mouse IL-12 R beta 1 Biotinylated Antibody Summary
Val32-Glu561
Accession # Q60837
Customers also Viewed
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-12 R beta 1
IL-12 R beta 1 is a 100 kDa type I transmembrane protein that belongs to the gp130/G‑CSF R family of cytokine receptors. IL-12 R beta 1 is a common subunit of both the IL‑12 and IL-23 receptor complexes which play distinct but related roles in T cell mediated inflammatory reactions (1, 2). Mature mouse IL-12 R beta 1 contains a 546 amino acid (aa) extracellular domain (ECD) with five fibronectin type III repeats, and a 147 aa cytoplasmic domain (3). Within the ECD, mouse IL-12 R beta 1 shares 85% and 52% aa sequence identity with rat and human IL-12 R beta 1, respectively. It shares 16%‑21% aa sequence identity with the ECDs of mouse gp130, LIF R, G‑CSF R, and IL‑23 R. IL-12 and IL-23 are disulfide linked heterodimeric cytokines that share a common p40 subunit (1, 2). IL-12 R beta 1 interacts with p40 at low affinity but does not transmit signals (3). Increased ligand binding affinity and signaling capacity are gained by association of IL-12 R beta 1 with either IL-12 R beta 2 or IL‑23 R (4‑6). IL‑12 R beta 2 and IL‑23 R are the signal transducing components of these receptor complexes (4, 7). IL-12 R beta 1 is expressed on activated T cells, NK cells, B cells, macrophages, and microglia (8‑10). IL-12 induced signaling promotes the development of naïve T cells into IFN-beta producing Th1 cells (11). IL-23 contributes to chronic inflammation by inducing the production of IL-17 by memory T cells (12). Naturally occurring homodimers of p40 can function as antagonists of IL-12 and IL‑23 and can also induce macrophage chemotaxis in the absence of IL-12 R beta 2 (13, 14).
- Becker, C. et al. (2005) Inflamm. Bowel Dis. 11:755.
- Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.
- Chua, A.O. et al. (1995) J. Immunol. 155:4286.
- Parham, C. et al. (2002) J. Immunol. 168:5699.
- Wu, C. et al. (1997) J. Immunol. 159:1658.
- Zou, J. et al. (1997) J. Biol. Chem. 272:6073.
- Presky, D.H. et al. (1996) Proc. Natl. Acad. Sci. 93:14002.
- Wu, C. et al. (1997) Eur. J. Immunol. 27:147.
- Airoldi, I. et al. (2000) J. Immunol. 165:6880.
- Li, J. et al. (2003) J. Neurol. Sci. 215:95.
- Schmitt, E. et al. (1994) Eur. J. Immunol. 24:793.
- Yen, D. et al. (2006) J. Clin. Invest. 116:1310.
- Shimozato, O. et al. (2006) Immunology 117:22.
- Russell, T.D. et al. (2003) J. Immunol. 171:6866.
Product Datasheets
Citations for Mouse IL-12 R beta 1 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
2
Citations: Showing 1 - 2
Filter your results:
Filter by:
-
Defining the functional binding sites of interleukin 12 receptor beta 1 and interleukin 23 receptor to Janus kinases
Authors: Doreen M. Floss, Tobias Klöcker, Jutta Schröder, Larissa Lamertz, Simone Mrotzek, Birgit Strobl et al.
Molecular Biology of the Cell
-
Tryptophan (W) at position 37 of murine IL-12/IL-23 p40 is mandatory for binding to IL-12Rbeta1 and subsequent signal transduction
Authors: J Georgy, Y Arlt, JM Moll, M Ouzin, HT Weitz, L Gremer, D Willbold, J Grötzinger, F Thives-Kur, J Scheller, DM Floss
The Journal of Biological Chemistry, 2021-10-09;0(0):101295.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsIsotype Controls
Reconstitution Buffers
Staining Reagents
Supplemental Cell Selection Products
Supplemental ELISA Products
Reviews for Mouse IL-12 R beta 1 Biotinylated Antibody
There are currently no reviews for this product. Be the first to review Mouse IL-12 R beta 1 Biotinylated Antibody and earn rewards!
Have you used Mouse IL-12 R beta 1 Biotinylated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
