Mouse IL-12 R beta 1 Biotinylated Antibody

Catalog #: BAF1998
2 Citations Datasheet / COA / SDS

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BAF1998

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Citations (2)

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Mouse IL-12 R beta 1 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-12 R beta 1 in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human IL-12 R beta 1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL-12 R beta 1
Val32-Glu561
Accession # Q60837

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse IL‑12 R beta 1 (Catalog # 1998-B1)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-12 R beta 1

IL-12 R beta 1 is a 100 kDa type I transmembrane protein that belongs to the gp130/G‑CSF R family of cytokine receptors. IL-12 R beta 1 is a common subunit of both the IL‑12 and IL-23 receptor complexes which play distinct but related roles in T cell mediated inflammatory reactions (1, 2). Mature mouse IL-12 R beta 1 contains a 546 amino acid (aa) extracellular domain (ECD) with five fibronectin type III repeats, and a 147 aa cytoplasmic domain (3). Within the ECD, mouse IL-12 R beta 1 shares 85% and 52% aa sequence identity with rat and human IL-12 R beta 1, respectively. It shares 16%‑21% aa sequence identity with the ECDs of mouse gp130, LIF R, G‑CSF R, and IL‑23 R. IL-12 and IL-23 are disulfide linked heterodimeric cytokines that share a common p40 subunit (1, 2). IL-12 R beta 1 interacts with p40 at low affinity but does not transmit signals (3). Increased ligand binding affinity and signaling capacity are gained by association of IL-12 R beta 1 with either IL-12 R beta 2 or IL‑23 R (4‑6). IL‑12 R beta 2 and IL‑23 R are the signal transducing components of these receptor complexes (4, 7). IL-12 R beta 1 is expressed on activated T cells, NK cells, B cells, macrophages, and microglia (8‑10). IL-12 induced signaling promotes the development of naïve T cells into IFN-beta producing Th1 cells (11). IL-23 contributes to chronic inflammation by inducing the production of IL-17 by memory T cells (12). Naturally occurring homodimers of p40 can function as antagonists of IL-12 and IL‑23 and can also induce macrophage chemotaxis in the absence of IL-12 R beta 2 (13, 14).

References

Becker, C. et al. (2005) Inflamm. Bowel Dis. 11:755.
Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.
Chua, A.O. et al. (1995) J. Immunol. 155:4286.
Parham, C. et al. (2002) J. Immunol. 168:5699.
Wu, C. et al. (1997) J. Immunol. 159:1658.
Zou, J. et al. (1997) J. Biol. Chem. 272:6073.
Presky, D.H. et al. (1996) Proc. Natl. Acad. Sci. 93:14002.
Wu, C. et al. (1997) Eur. J. Immunol. 27:147.
Airoldi, I. et al. (2000) J. Immunol. 165:6880.
Li, J. et al. (2003) J. Neurol. Sci. 215:95.
Schmitt, E. et al. (1994) Eur. J. Immunol. 24:793.
Yen, D. et al. (2006) J. Clin. Invest. 116:1310.
Shimozato, O. et al. (2006) Immunology 117:22.
Russell, T.D. et al. (2003) J. Immunol. 171:6866.

Long Name

Interleukin 12 Receptor beta 1

Entrez Gene IDs

3594 (Human); 16161 (Mouse)

Alternate Names

CD212 antigen; CD212; IL-12 R beta 1; IL-12 receptor beta component; IL-12 receptor subunit beta-1; IL12R beta 1; IL-12R subunit beta-1; IL12R; IL12RB; IL12RB1; IL-12Rb1; IL-12R-BETA1; IL-12R-beta-1; interleukin 12 receptor, beta 1; interleukin-12 receptor beta-1 chain; interleukin-12 receptor subunit beta-1; MGC34454

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Citations for Mouse IL-12 R beta 1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Defining the functional binding sites of interleukin 12 receptor beta 1 and interleukin 23 receptor to Janus kinases
Authors: Doreen M. Floss, Tobias Klöcker, Jutta Schröder, Larissa Lamertz, Simone Mrotzek, Birgit Strobl et al.
Molecular Biology of the Cell
Tryptophan (W) at position 37 of murine IL-12/IL-23 p40 is mandatory for binding to IL-12Rbeta1 and subsequent signal transduction
Authors: J Georgy, Y Arlt, JM Moll, M Ouzin, HT Weitz, L Gremer, D Willbold, J Grötzinger, F Thives-Kur, J Scheller, DM Floss
The Journal of Biological Chemistry, 2021-10-09;0(0):101295.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot