Mouse IL-17E/IL-25 Antibody

Catalog #: MAB13992
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB13992
MAB13992-SP

CXCL1/GRO alpha Secretion Induced by IL‑17E/IL‑25 and Neutralization by Mouse IL‑17E/IL‑25 Antibody.

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Citations (2)

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Mouse IL-17E/IL-25 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL‑17E/IL‑25 in ELISAs.

Source

Monoclonal Rat IgG_2B Clone # 881014

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse IL‑17E/IL‑25
Tyr17-Ala169
Accession # NP_542767

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Neutralization

Measured by its ability to neutralize IL‑17E/IL‑25-induced CXCL1/GRO alpha secretion in the HT‑29 human colon adenocarcinoma cell line. The Neutralization Dose (ND₅₀) is typically 0.005-0.025 μg/mL in the presence of 10 ng/mL Recombinant Mouse IL‑17E/IL‑25.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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CXCL1/GRO alpha Secretion Induced by IL‑17E/IL‑25 and Neutralization by Mouse IL‑17E/IL‑25 Antibody. Recombinant Mouse IL-17E/IL-25 (Catalog # 1399-IL) induces CXCL1/GROa secretion in the the HT-29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by Human CXCL1/GROa DuoSet (Catalog # DY275). CXCL1/GROa Secretion elicited by Recombinant Mouse IL-17E/IL-25 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-17E/IL-25 Monoclonal Antibody (Catalog # MAB13992). The ND₅₀ is typically 0.005-0.025 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17E/IL-25

The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus. With the exception of IL-17B, which exists as a non-covalently linked dimer, all IL-17 family members are disulfide-linked dimers. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2). Mouse IL-17E cDNA encodes a 169 amino acid (aa) residues precursor protein with a putative 16 aa signal peptide (5). Mature mouse IL-17E shares 76% and 91% amino sequence (aa) identity with mature human and rat IL-17E, respectively. Mouse IL-17E also shares from 24% to 32% sequence identity with the other mouse IL‑17 family members. IL-17E expression was detected at very low levels by PCR in various peripheral tissues including brain, kidney, lung, prostate, testis, adrenal gland spinal cord, and trachea. IL-17E binds and activates IL-17 B Receptor (IL-17B R) (alternatively known as IL-17 Rh1, IL-17E R, and EVI27) (3, 4), which is expressed in kidney and liver, and at lower levels in brain, testis and other endocrine tissues. The expression of IL-17B R is up regulated under inflammatory conditions. Ligation of IL-17E to IL-17 RB induces activation of nuclear factor kappa-B and stimulates the production of the proinflamatory cytokine IL-8 (3). IL-17 has also been found to promote the expression of the prototypical Th2 genes (4, 5).

References

Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
Lee, J. et al. (2001) J. Biol. Chem. 276:1660.
Hurst, S.D. et al. (2002) J. Immunol. 169:443.
Pan, G. et al. (2001) J. Immunol. 167:6569.

Long Name

Interleukin 17E

Entrez Gene IDs

64806 (Human); 140806 (Mouse); 501996 (Rat)

Alternate Names

IL17E; IL-17E; IL25; IL-25; interleukin 25; Interleukin-17E; interleukin-25

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Mouse IL-17E/IL-25 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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A tuft cell - ILC2 signaling circuit provides therapeutic targets to inhibit gastric metaplasia and tumor development
Authors: O'Keefe, RN;Carli, ALE;Baloyan, D;Chisanga, D;Shi, W;Afshar-Sterle, S;Eissmann, MF;Poh, AR;Pal, B;Seillet, C;Locksley, RM;Ernst, M;Buchert, M;
Nature communications
Species: Mouse
Sample Types: In Vivo, Organoids
Applications: In Vivo, Neutralization
Eosinophil extracellular traps drive asthma progression through neuro-immune signals
Authors: Y Lu, Y Huang, J Li, J Huang, L Zhang, J Feng, J Li, Q Xia, Q Zhao, L Huang, S Jiang, S Su
Nature Cell Biology, 2021-10-06;23(10):1060-1072.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo