Mouse IL-1 beta /IL-1F2 Antibody Summary
Val118-Ser269
Accession # P10749
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse IL‑1 beta /IL‑1F2 by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with LPS. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB4011) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-1 beta /IL-1F2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Mouse IL-1 beta/IL-1F2 by Western Blot Pleural cells are involved in bleomycin-induced inflammation. a Cell recruitment in PLF was assessed by total cell count at D3, D14 and D21. Results are expressed as mean ± SEM. b Inflammation was estimated by differential count in PLF after May-Grünwald Giemsa staining. Results are expressed as percentages of total cells. n = 4 for NaCl groups, n = 6 for BLM groups at D3 and D21, n = 6 for NaCl and n = 8 for BLM at D14. c, d Caspase-1 activation was assessed at D14 by c western blot in whole lung extracts (n = 3 mice/group) or by d specific caspase-1 FLICA analysis on lung section from NaCl or BLM injected mice (Representative images are shown. Active caspase-1: green, DAPI: blue, dotted lines represent the pleura, scale bars: 50 μm). e, f Met5A cells were treated with BLM (100 μM) and e activation of caspase-1 was followed (western blot; left, densitometric analysis; right). f active IL-1 beta was assessed in the supernatant of Met5A cells after 24 h of culture. Representative results from 3 independent experiments. HSC70 serves as a loading control. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 Image collected and cropped by CiteAb from the following publication (https://respiratory-research.biomedcentral.com/articles/10.1186/s12931-016-0475-8), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 beta/IL-1F2
IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 67%-78% with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.
Product Datasheets
Citations for Mouse IL-1 beta /IL-1F2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Inflammasome Activation by Adenylate Cyclase Toxin Directs Th17 Responses and Protection against Bordetella pertussis
Authors: Aisling Dunne, Pádraig J. Ross, Eva Pospisilova, Jiri Masin, Aoife Meaney, Caroline E. Sutton et al.
The Journal of Immunology
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Inhibition of the NLRP3 inflammasome can prevent sterile intra-amniotic inflammation, preterm labor/birth, and adverse neonatal outcomes†
Authors: Nardhy Gomez-Lopez, Roberto Romero, Valeria Garcia-Flores, Yaozhu Leng, Derek Miller, Sonia S Hassan et al.
Biology of Reproduction
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Intra-amniotic inflammation induces preterm birth by activating the NLRP3 inflammasome†
Authors: Jonathan Faro, Roberto Romero, George Schwenkel, Valeria Garcia-Flores, Marcia Arenas-Hernandez, Yaozhu Leng et al.
Biology of Reproduction
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Macrophage- and RIP3-dependent inflammasome activation exacerbates retinal detachment-induced photoreceptor cell death
Authors: K Kataoka, H Matsumoto, H Kaneko, S Notomi, K Takeuchi, J H Sweigard et al.
Cell Death & Disease
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Pleural inhibition of the caspase-1/IL-1? pathway diminishes profibrotic lung toxicity of bleomycin
Respir. Res, 2016-11-29;17(1):162.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Porphyromonas gingivalis-derived lysine gingipain enhances osteoclast differentiation induced by tumor necrosis factor-alpha and interleukin-1beta but suppresses that by interleukin-17A: importance of proteolytic degradation of osteoprotegerin by lysine gingipain.
Authors: Akiyama T, Miyamoto Y, Yoshimura K, Yamada A, Takami M, Suzawa T, Hoshino M, Imamura T, Akiyama C, Yasuhara R, Mishima K, Maruyama T, Kohda C, Tanaka K, Potempa J, Yasuda H, Baba K, Kamijo R
J Biol Chem, 2014-04-22;289(22):15621-30.
Species: Bacteria - Porphyromonas gingivalis
Sample Types: Recombinant Protein
Applications: Western Blot -
Inflammasome activation by altered proteostasis.
Authors: Shin, Jin Na, Fattah, Elmoataz, Bhattacharya, Abhisek, Ko, Soyoung, Eissa, N Tony
J Biol Chem, 2013-10-31;288(50):35886-95.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Chemotherapy-triggered cathepsin B release in myeloid-derived suppressor cells activates the Nlrp3 inflammasome and promotes tumor growth.
Authors: Bruchard M, Mignot G, Derangere V, Chalmin F, Chevriaux A, Vegran F, Boireau W, Simon B, Ryffel B, Connat J, Kanellopoulos J, Martin F, Rebe C, Apetoh L, Ghiringhelli F
Nat Med, 2012-12-02;19(1):57-64.
Species: Mouse
Sample Types: Serum
Applications: Immunoprecipitation, Western Blot -
Caspase-1, caspase-8, and calpain are dispensable for IL-33 release by macrophages.
Authors: Ohno T, Oboki K, Kajiwara N, Morii E, Aozasa K, Flavell RA, Okumura K, Saito H, Nakae S
J. Immunol., 2009-12-15;183(12):7890-7.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Metallothionein 3-Zinc Axis Suppresses Caspase-11 Inflammasome Activation and Impairs Antibacterial Immunity
Authors: Debabrata Chowdhury, Jason C. Gardner, Abhijit Satpati, Suba Nookala, Santhosh Mukundan, Aleksey Porollo et al.
Frontiers in Immunology
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