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Mouse IL-1ra/IL-1F3 Biotinylated Antibody

Catalog #: BAF480
4 Citations Datasheet / COA / SDS
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BAF480
Product Details
Citations (4)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-1ra/IL-1F3 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL‑1ra/IL-1F3 in ELISAs and Western blots. In sandwich immunoassays, approximately 45% cross-reactivity with recombinant rat IL‑1ra is observed and less than 1% cross-reactivity with recombinant human IL-1ra, recombinant porcine IL-1ra, and recombinant equine IL‑1ra is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse IL‑1ra/IL-1F3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IL‑1ra/IL‑1F3 (Catalog # 480-RM)

Mouse IL-1ra/IL-1F3 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse IL‑1ra/IL‑1F3 Antibody (Catalog # AF-480-NA)

Standard: Recombinant Mouse IL-1ra/IL-1F3 Protein (Catalog # 480-RM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1ra/IL-1F3

IL-1ra was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The naturally-occurring, fully glycosylated form has an apparent molecular weight of about 25,000 Daltons. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha. It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptor (I and II) on human cells. In mouse, IL-1 RII does not bind IL-1ra. IL-1ra has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Pre-clinical and clinical studies were done to test possible therapeutic applications for IL-1ra in the treatment of sepsis, rheumatoid arthritis and chronic myelogenous leukemia.

Long Name
Interleukin 1 Receptor Antagonist
Entrez Gene IDs
3557 (Human); 16181 (Mouse); 60582 (Rat); 397499 (Porcine)
Alternate Names
DIRA; ICIL-1ra; IL1F3; IL-1F3; IL1ra; IL-1ra; IL-1ra3; IL1RN; IL-1RN; interleukin 1 receptor antagonist; IRAP; MVCD4

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Citations for Mouse IL-1ra/IL-1F3 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. FcgammaR expression on macrophages is related to severity and chronicity of synovial inflammation and cartilage destruction during experimental immune-complex-mediated arthritis (ICA)
    Authors: Arjen B Blom, Peter L van Lent, Hanneke van Vuuren, Astrid E Holthuysen, Cor Jacobs, Leo B van de Putte et al.
    Arthritis Res
  2. Stratification is the key: inflammatory biomarkers accurately direct immunomodulatory therapy in experimental sepsis.
    Authors: Osuchowski MF, Connett J, Welch K, Granger J, Remick DG
    Crit. Care Med., 2009-05-01;37(5):1567-73.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development
  3. Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
    Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG
    J. Immunol., 2006-08-01;177(3):1967-74.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development
  4. Sequential ELISA to profile multiple cytokines from small volumes.
    Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
    J. Immunol. Methods, 2005-07-01;302(1):172-81.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development

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