Home / IL-2 / Mouse IL-2 Antibody

Mouse IL-2 Antibody

Catalog #: MAB702
16 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB702-SP
MAB702-100
MAB702-500
Cell Proliferation Induced by IL‑2 and Neutralization by Mouse IL‑2 Antibody.
1 Image
Product Details
Citations (16)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-2 in ELISAs.
Source
Monoclonal Rat IgG1 Clone # JES6-1A12
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse IL-2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample

Mouse IL-2 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse IL‑2 Biotinylated Antibody (Catalog # BAF402)

Standard: Recombinant Mouse IL-2 Protein (Catalog # 402-ML)

Neutralization
Measured by its ability to neutralize IL‑2-induced proliferation in the CTLL‑2 mouse cytotoxic T cell line. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 276. The Neutralization Dose (ND50) is typically 0.125-0.75 µg/mL in the presence of 1 ng/mL Recombinant Mouse IL‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑2 and Neutralization by Mouse IL‑2 Antibody. View Larger

Cell Proliferation Induced by IL‑2 and Neutralization by Mouse IL‑2 Antibody. Recombinant Mouse IL-2 (Catalog # 402-ML) stimulates proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL-2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-2 Monoclonal Antibody (Catalog # MAB702). The ND50 is typically 0.125-0.75 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-2

IL-2 is a cytokine that stimulates the growth and differentiation of B cells, T cells, NK cells, and monocyte/macrophages. It functions through the heterotrimeric IL-2 receptor comprising alpha, beta, and gamma  chains.

Long Name
Interleukin 2
Entrez Gene IDs
3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
Alternate Names
Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; Proleukin; T cell growth factor; T-cell growth factor; TCGF

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Mouse IL-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. IL-1beta promotes TGF-beta1 and IL-2 dependent Foxp3 expression in regulatory T cells.
    Authors: Ganesh BB, Bhattacharya P, Gopisetty A, Sheng J, Vasu C, Prabhakar BS
    PLoS ONE, 2011-07-11;6(7):e21949.
  2. Regulation of the bi-directional cross-talk between ovarian cancer cells and adipocytes by SPARC
    Authors: B John, C Naczki, C Patel, A Ghoneum, S Qasem, Z Salih, N Said
    Oncogene, 2019-02-14;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  3. The Xenobiotic Transporter Mdr1 Enforces T Cell Homeostasis in the Presence of Intestinal Bile Acids
    Authors: Wei Cao, Hisako Kayama, Mei Lan Chen, Amber Delmas, Amy Sun, Sang Yong Kim et al.
    Immunity
  4. mTORC1-dependent AMD1 regulation sustains polyamine metabolism in prostate cancer
    Authors: Amaia Zabala-Letona, Amaia Arruabarrena-Aristorena, Natalia Martín-Martín, Sonia Fernandez-Ruiz, James D. Sutherland, Michelle Clasquin et al.
    Nature
  5. Metabolic control of type 1 regulatory (Tr1) cell differentiation by AHR and HIF1-alpha
    Authors: Ivan D. Mascanfroni, Maisa C. Takenaka, Ada Yeste, Bonny Patel, Yan Wu, Jessica E. Kenison et al.
    Nature Medicine
  6. Interleukin-7 optimizes FOXP3+CD4+ regulatory T cells reactivity to interleukin-2 by modulating CD25 expression.
    Authors: Simonetta F, Gestermann N, Bloquet S, Bourgeois C
    PLoS ONE, 2014-12-08;9(12):e113314.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Adoptive Transfer
  7. Aiolos promotes TH17 differentiation by directly silencing Il2 expression
    Authors: Francisco J Quintana, Hulin Jin, Evan J Burns, Meghan Nadeau, Ada Yeste, Deepak Kumar et al.
    Nature Immunology
  8. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012-01-25;58(2):169-77.
    Species: Mouse
    Sample Types: Plasma
    Applications: Luminex Development
  9. Naive and activated T cells display differential responsiveness to TL1A that affects Th17 generation, maintenance, and proliferation.
    Authors: Jones GW, Stumhofer JS, Foster T, Twohig JP, Hertzog P, Topley N, Williams AS, Hunter CA, Jenkins BJ, Wang EC, Jones SA
    FASEB J., 2010-09-08;25(1):409-19.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Loss of CD4+ T cell IL-6R expression during inflammation underlines a role for IL-6 trans signaling in the local maintenance of Th17 cells.
    Authors: Jones GW, McLoughlin RM, Hammond VJ, Parker CR, Williams JD, Malhotra R, Scheller J, Williams AS, Rose-John S, Topley N, Jones SA
    J. Immunol., 2010-01-18;184(4):2130-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Role of Valpha14+ NKT cells in the development of Hepatitis B virus-specific CTL: activation of Valpha14+ NKT cells promotes the breakage of CTL tolerance.
    Authors: Ito H, Ando K, Ishikawa T
    Int. Immunol., 2008-05-16;20(7):869-79.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  12. Tumor-specific Th17-polarized cells eradicate large established melanoma.
    Authors: Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP
    Blood, 2008-03-19;112(2):362-73.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  13. Attenuation of autoimmune disease in Fas-deficient mice by treatment with a cytotoxic benzodiazepine.
    Authors: Bednarski JJ, Warner RE, Rao T
    Arthritis Rheum., 2003-03-01;48(3):757-66.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISpot Development
  14. Discovery and characterization of a monoclonal antibody targeting a conformational epitope of IL-6/IL-6R alpha to inhibit IL-6/ IL-6R alpha /gp130 hexameric signaling complex formation
    Authors: Chun-Chi Chou, Kuo-Tai Hua, Min-Wei Chen, Chin-Jui Wu, Chun-Hua Hsu, Jann-Tay Wang et al.
    mAbs
  15. Potentiation of T Cell Stimulatory Activity by Chemical Fixation of a Weak Peptide-MHC Complex
    Authors: I Hwang, K Kim, S Choi, M Lomunova
    Mol. Cells, 2017-01-31;40(1):24-36.
  16. Regulatory T Cells Condition Lymphatic Endothelia for Enhanced Transendothelial Migration
    Authors: W Piao, Y Xiong, L Li, V Saxena, KD Smith, KL Hippen, C Paluskievi, M Willsonshi, BR Blazar, R Abdi, JS Bromberg
    Cell Rep, 2020-01-28;30(4):1052-1062.e5.

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Mouse IL-2 Antibody

There are currently no reviews for this product. Be the first to review Mouse IL-2 Antibody and earn rewards!

Have you used Mouse IL-2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review