Mouse IL-22BP Antibody

Catalog #: AF2376
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2376
AF2376-SP

All IL-22BP Antibodies

Product Details

Citations (2)

Supplemental Products

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-22BP Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-22BP in direct ELISAs and Western blots. In Western blots, approximately 10% cross‑reactivity with recombinant human (rh) IL-22BP is observed and approximately 5% cross-reactivity with rhIL-22 R is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL‑22BP
Thr19-Pro230 (Asp149Glu)
Accession # Q80XF5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse IL‑22BP Fc Chimera (Catalog # 2376-BP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-22BP

Interleukin 22 binding protein (IL-22BP), also known as CRF2-10, CRF2-X, and IL-22 RA2, is a 35‑45 kDa secreted glycoprotein belonging to the type II cytokine receptor family (CRF). IL-22 signals through a receptor complex consisting of IL-22 R and IL-10 R beta. IL-10 R beta is also a component of the receptor complexes for IL‑10, IL-26, IL-28, and IL-29 (1‑4). IL‑10 R beta is widely expressed, and other cell types express either IL-10 R alpha or IL-22 R but not both (5). The mouse IL-22BP cDNA encodes a 230 amino acid (aa) precursor that includes a 20 aa signal sequence and two fibronectin type-III domains (6). Mouse IL-22BP shares 59% and 85% aa sequence identity with human and rat IL-22BP, respectively. Among type II cytokine receptors, IL-22BP is most closely related to the extracellular domain of IL-22 R, sharing 36% aa sequence identity. Splice variants of human IL-22BP exist but have not been detected for the mouse protein (6‑8). IL-22BP binds an epitope on IL-22 that is required for the interaction of IL-22 with the transmembrane IL-22 R (8‑10), thereby preventing the association of IL-22 R with IL-10 R beta (10). IL-22BP blocks IL‑22‑induced responses including induction of MHC class I expression, Stat1 and Stat3 activation, SOCS3 gene expression,the production of reactive oxygen species (6, 9) and the modulation of the proinflammatory effects of IL-22 (11). Mouse IL-22 BP neutralizes both mouse and human IL-22 (6). IL-22BP is secreted by epithelial cells, dendritic cells, activated B cells, and activated monocytes (6, 8, 12, 13).

References

Donnelly, R.P. et al. (2004) J. Leukoc. Biol. 76:314.
Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
Kotenko, S.V. (2002) Cytokine Growth Factor Rev. 13:223.
Wolk, K. et al. (2005) Genes Immun. 6:8.
Wei, C.-C. et al. (2003) Genes Immun. 4:204.
Dumoutier, L. et al. (2001) J. Immunol. 166:7090.
Xu, W. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9511.
Kotenko, S.V. et al. (2001) J. Immunol. 166:7096.
Li, J. et al. (2004) Int. Immunopharmacol. 4:693.
Whittington, H.A. et al. (2004) Am. J. Respir. Cell Mol. Biol. 31:220.
Lecart, S. et al. (2002) Int. Immunol. 14:1351.
Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577.

Long Name

Interleukin 22 Binding Protein

Entrez Gene IDs

116379 (Human); 237310 (Mouse)

Alternate Names

class II cytokine receptor; CRF2-10; CRF2-S1IL22BP; CRF2-X; Cytokine receptor class-II member 10; Cytokine receptor family 2 member 10; Cytokine receptor family type 2, soluble 1; IL-22 RA2; IL-22 receptor subunit alpha-2; IL22BP; IL-22BP; IL-22BPCRF2X; IL22RA2; IL-22RA2; IL-22R-alpha-2; interleukin 22 receptor, alpha 2; interleukin 22-binding protein; interleukin-22 receptor subunit alpha-2; Interleukin-22-binding protein; MGC150509; MGC150510; zcytoR16

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Mouse IL-22BP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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