Mouse IL-33 Biotinylated Antibody Summary
Ser109-Ile266
Accession # Q8BVZ5
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Applications
Mouse IL-33 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-33
IL-33, also known as NF-HEV and DVS 27, is a 30 kDa proinflammatory protein that may also regulate gene transcription (1-3). DVS 27 was identifed as a gene that is upregulated in vasospastic cerebral arteries (1). NF-HEV was described as a nuclear factor that is preferentially expressed in the endothelial cells of high endothelial venules relative to endothelial cells from other tissues (2). IL-33 was identified based on sequence and structural homology with IL-1 family cytokines (3). DVS 27, NF-HEV, and IL-33 share 100% amino acid (aa) sequence identity. IL-33 is constitutively expressed in smooth muscle and airway epithelia. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL-1 beta stimulation (1, 3). Similar to IL-1, IL-33 can be cleaved in vitro by caspase-1, generating an N-terminal fragment that is slightly shorter than the C-terminal fragment (3, 4). The N-terminal portion of full length IL-33 contains a predicted bipartite nuclear localization sequence and a homeodomain-like helix-turn-helix DNA binding domain. By immunofluorescence, full length IL-33 localizes to the nucleus in HUVECs and transfectants (2). The C-terminal fragment, corresponding to mature IL-33, binds and triggers signaling through mast cell IL-1 R4/ST2L, a longtime orphan receptor involved in the augmentation of Th2 cell responses (3, 5-7). A ternary signaling complex is formed by the subsequent association of IL-33 and ST2L with IL-1R AcP (8). Stimulation of Th2 polarized lymphocytes with mature IL-33 in vitro induces IL-5 and IL-13 secretion (3). In vivo administration of mature IL-33 promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues (3). Full length and mature mouse IL-33 share approximately 55% and 90% aa sequence identity with human and rat IL-33, respectively. Mouse IL-33 shares less than 25% aa sequence identity with other IL-1 family proteins.
- Onda, H. et al. (1999) J. Cereb. Blood Flow Metab. 19:1279.
- Baekkevold, E.S. et al. (2003) Am. J. Pathol. 163:69.
- Schmitz, J. et al. (2005) Immunity 23:479.
- Black, R.A. et al. (1989) J. Biol. Chem. 264:5323.
- Xu, D. et al. (1998) J. Exp. Med. 187:787.
- Lohning, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:6930.
- Dinarello, C.A. (2005) Immunity 23:461.
- Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
Product Datasheets
Citations for Mouse IL-33 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
11
Citations: Showing 1 - 10
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Epithelial STAT6 O-GlcNAcylation drives a concerted anti-helminth alarmin response dependent on tuft cell hyperplasia and Gasdermin C
Authors: Ming Zhao, Kaiqun Ren, Xiwen Xiong, Yue Xin, Yujie Zou, Jason C. Maynard et al.
Immunity
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FAK regulates IL-33 expression by controlling chromatin accessibility at c-Jun motifs
Authors: BGC Griffith, R Upstill-Go, H Brunton, GR Grimes, AV Biankin, B Serrels, A Byron, MC Frame
Scientific Reports, 2021-01-08;11(1):229.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
Cell. Physiol. Biochem., 2017-08-09;42(5):1961-1972.
Species: Mouse
Sample Types: Serum
Applications: ELISA Development (Detection) -
Pulmonary Epithelial Cell-Derived Cytokine TGF-beta1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function.
Authors: Denney L, Byrne A, Shea T, Buckley J, Pease J, Herledan G, Walker S, Gregory L, Lloyd C
Immunity, 2015-11-17;43(5):945-58.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Development (Detection) -
AMCase is a crucial regulator of type 2 immune responses to inhaled house dust mites.
Authors: Kim L, Morita R, Kobayashi Y, Eisenbarth S, Lee C, Elias J, Eynon E, Flavell R
Proc Natl Acad Sci U S A, 2015-05-18;112(22):E2891-9.
Species: Mouse
Sample Types: BALF
Applications: ELISA Development (Detection) -
IL-33 is required for disposal of unnecessary cells during ovarian atresia through regulation of autophagy and macrophage migration.
Authors: Wu J, Carlock C, Zhou C, Nakae S, Hicks J, Adams H, Lou Y
J Immunol, 2015-01-23;194(5):2140-7.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Severe neutrophil-dominated inflammation and enhanced myelopoiesis in IL-33-overexpressing CMV/IL33 mice.
Authors: Talabot-Ayer D, Martin P, Vesin C, Seemayer C, Vigne S, Gabay C, Palmer G
J Immunol, 2014-12-10;194(2):750-60.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Unique temporal and spatial expression patterns of IL-33 in ovaries during ovulation and estrous cycle are associated with ovarian tissue homeostasis.
Authors: Carlock C, Wu J, Zhou C, Tatum K, Adams H, Tan F, Lou Y
J Immunol, 2014-05-23;193(1):161-9.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Bee venom phospholipase A2 induces a primary type 2 response that is dependent on the receptor ST2 and confers protective immunity.
Authors: Palm N, Rosenstein R, Yu S, Schenten D, Florsheim E, Medzhitov R
Immunity, 2013-10-24;39(5):976-85.
Species: Human, Mouse
Sample Types: Cell Culture Supernates, Peritoneal Fluid
Applications: ELISA Development (Detection) -
Evidence for a multipotent mammary progenitor with pregnancy-specific activity
Authors: Alice S Kaanta, Carl Virtanen, Laura M Selfors, Joan S Brugge, Benjamin G Neel
Breast Cancer Research
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Induction of IL-33 expression and activity in central nervous system glia.
Authors: Hudson CA, Christophi GP, Gruber RC, Wilmore JR, Lawrence DA, Massa PT
J. Leukoc. Biol., 2008-06-13;84(3):631-43.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development
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