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Mouse IL-5 DuoSet ELISA

Catalog #: DY405
80 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY405
DY405-05
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Mouse IL-5 ELISA Standard Curve
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Product Details
Procedure
Citations (80)
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Supplemental Products
Reviews (1)
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Mouse IL-5 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
31.2 - 2,000 pg/mL
Sufficient Materials
For five or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse IL-5. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

N/A Mouse IL-5 ELISA Standard Curve View Larger

Mouse IL-5 ELISA Standard Curve

Product Datasheets

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Product Datasheet
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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-5

IL-5 (Interleukin-5) is a secreted cytokine that is expressed as a covalent dimer. It is produced by activated Th2 cells, eosinophils, mast cells, and iNKT cells and promotes eosinophilic and basophilic differentiation and activation. IL-5 signals through a receptor complex containing IL-5 R alpha and the Common beta Chain/CD131 on eosinophils and basophils. Naturally occurring soluble forms of IL-5 R alpha retain the ability to bind IL-5 and limit its bioavailability.

Long Name:
Interleukin 5
Entrez Gene IDs:
3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)
Alternate Names:
BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Mouse IL-5 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

80 Citations: Showing 1 - 10
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  1. Host gastric corpus microenvironment facilitates Ascaris suum larval hatching and infection in a murine model
    Authors: Wu, Y;Adeniyi-Ipadeola, G;Adkins-Threats, M;Seasock, M;Suarez-Reyes, C;Fujiwara, R;Bottazzi, ME;Song, L;Mills, JC;Weatherhead, JE;
    PLoS neglected tropical diseases
  2. Microbiota encoded fatty-acid metabolism expands tuft cells to protect tissues homeostasis during Clostridioides difficile infection in the large intestine
    Authors: Kellogg, TD;Ceglia, S;Mortzfeld, BM;Zeamer, AL;Foley, SE;Ward, DV;Bhattarai, SK;McCormick, BA;Reboldi, A;Bucci, V;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Tissue Homogenates
  3. Cullin5 drives experimental asthma exacerbations by modulating alveolar macrophage antiviral immunity
    Authors: Zhang, H;Xue, K;Li, W;Yang, X;Gou, Y;Su, X;Qian, F;Sun, L;
    Nature communications
    Species: Mouse, Transgenic Mouse
    Sample Types: BALF
  4. Acrylamide, an Air Pollutant, Enhances Allergen-Induced Eosinophilic Lung Inflammation via Group 2 Innate Lymphoid Cells
    Authors: Su, HH;Cheng, CM;Yang, YN;Chang, YW;Li, CY;Wu, ST;Lin, CC;Wu, HE;Suen, JL;
    Mucosal immunology
    Species: Mouse
    Sample Types: BALF, Cell Culture Supernates
  5. TRAF4 is crucial for the propagation of ST2+ memory Th2 cells in IL-33-mediated type 2 airway inflammation
    Authors: J Xiao, X Chen, W Liu, W Qian, K Bulek, L Hong, W Miller-Lit, X Li, C Liu
    bioRxiv : the preprint server for biology, 2023-02-12;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  6. IL-4/IL-13-producing ILC2s are required for timely control of intestinal helminth infection in mice
    Authors: F Varela, C Symowski, J Pollock, S Wirtz, D Voehringer
    European Journal of Immunology, 2022-09-28;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  7. Inhaled delivery of recombinant interferon-lambda restores allergic inflammation after development of asthma by controlling Th2- and Th17-cell-mediated immune responses
    Authors: J Won, A Jo, CH Gil, S Kim, H Shin, H Jik Kim
    International immunopharmacology, 2022-08-27;112(0):109180.
    Species: Mouse
    Sample Types: BALF
  8. Phosphatidylinositol 3-Kinase (PI3K) Orchestrates Aspergillus fumigatus-Induced Eosinophil Activation Independently of Canonical Toll-Like Receptor (TLR)/C-Type-Lectin Receptor (CLR) Signaling
    Authors: A Dietschman, S Schruefer, S Westermann, F Henkel, K Castiglion, R Willebrand, J Adam, J Ruland, R Lang, DC Sheppard, J Esser-von-, D Radtke, S Krappmann, D Voehringer
    MBio, 2022-06-13;0(0):e0123922.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  9. Macrophage-intrinsic DUOX1 contributes to type 2 inflammation and mucus metaplasia during allergic airway disease
    Authors: CR Morris, A Habibovic, CM Dustin, C Schiffers, MC Lin, JL Ather, YMW Janssen-He, ME Poynter, O Utermohlen, M Krönke, A van der Vl
    Mucosal Immunology, 2022-06-02;0(0):.
    Species: Mouse
    Sample Types: BALF
  10. Exogenous leptin enhances markers of airway fibrosis in a mouse model of chronic allergic airways disease
    Authors: MD Ihrie, VL McQuade, JT Womble, A Hegde, MS McCravy, CVG Lacuesta, RM Tighe, LG Que, JKL Walker, JL Ingram
    Respiratory Research, 2022-05-24;23(1):131.
    Species: Mouse
    Sample Types: BALF
  11. Menthone Inhalation Alleviates Local and Systemic Allergic Inflammation in Ovalbumin-Sensitized and Challenged Asthmatic Mice
    Authors: YH Su, JY Lin
    International Journal of Molecular Sciences, 2022-04-04;23(7):.
    Species: Mouse
    Sample Types: BALF
  12. Resveratrol attenuates inflammation and apoptosis through alleviating endoplasmic reticulum stress via Akt/mTOR pathway in fungus-induced allergic airways inflammation
    Authors: S Wang, L Gong, Y Mo, J Zhang, Z Jiang, Z Tian, C Shao
    International immunopharmacology, 2021-12-27;103(0):108489.
    Species: Mouse
    Sample Types: BALF
  13. Transient Ascaris suum larval migration induces intractable chronic pulmonary disease and anemia in mice
    Authors: Y Wu, E Li, M Knight, G Adeniyi-Ip, LZ Song, AR Burns, AC Gazzinelli, R Fujiwara, ME Bottazzi, JE Weatherhea
    PloS Neglected Tropical Diseases, 2021-12-16;15(12):e0010050.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  14. DRP1-Mediated Mitochondrial Fission Regulates Lung Epithelial Response to Allergen
    Authors: SR Bruno, A Kumar, ZF Mark, R Chandrasek, E Nakada, N Chamberlai, B Mihavics, J Walzer, J Cahoon, AE Dixon, B Cunniff, V Anathy
    International Journal of Molecular Sciences, 2021-10-15;22(20):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  15. Fasting impairs type 2 helper T cell infiltration in the lung of an eosinophilic asthma mouse model
    Authors: Y Suzuki, T Hayashi, R Yokoyama, F Nakagawa, J Inoue, T Higashi, R Onodera, K Motoyama
    FEBS Open Bio, 2021-08-20;0(0):.
    Species: Mouse
    Sample Types: BALF
  16. Ameliorative effects of eosinophil deficiency on immune response, endoplasmic reticulum stress, apoptosis, and autophagy in fungus-induced allergic lung inflammation
    Authors: S Wang, Z Jiang, L Li, J Zhang, C Zhang, C Shao
    Respiratory Research, 2021-06-07;22(1):173.
    Species: Mouse
    Sample Types: BALF
  17. Development of Delivery Systems Enhances the Potency of Cell-Based HIV-1 Therapeutic Vaccine Candidates
    Authors: A Hadi, A Rastgoo, M Eskandaria, N Haghighipo, A Bolhassani
    Journal of Immunology Research, 2021-04-20;2021(0):5538348.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  18. Sprouty2 positively regulates T cell function and airway inflammation through regulation of CSK and LCK kinases
    Authors: A Sripada, K Sirohi, L Michalec, L Guo, JT McKay, S Yadav, M Verma, J Good, D Rollins, MM Gorska, R Alam
    PloS Biology, 2021-03-08;19(3):e3001063.
    Species: Mouse
    Sample Types: BALF
  19. Wood emissions and asthma development: Results from an experimental mouse model and a prospective cohort study
    Authors: KM Junge, L Buchenauer, E Elter, K Butter, T Kohajda, G Herberth, S Röder, M Borte, W Kiess, M von Bergen, JC Simon, UE Rolle-Kamp, I Lehmann, R Gminski, M Ohlmeyer, T Polte
    Environment international, 2021-02-18;151(0):106449.
    Species: Mouse
    Sample Types: Tissue Homogenates
  20. Interleukin (IL)-33 is dispensable for Schistosoma mansoni worm maturation and the maintenance of egg-induced pathology in intestines of infected mice
    Authors: JPK Mukendi, R Nakamura, S Uematsu, S Hamano
    Parasites & vectors, 2021-01-22;14(1):70.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  21. High-dose intranasal application of titanium dioxide nanoparticles induces the systemic uptakes and allergic airway inflammation in asthmatic mice
    Authors: S Abdulnasse, M Hannig, DD Le, S Heck, M Leitner, AJ Omlor, I Tavernaro, A Kraegeloh, R Kautenburg, G Kickelbick, A Beilhack, M Bischoff, J Nguyen, M Sester, R Bals, QT Dinh
    Respir. Res., 2020-07-02;21(1):168.
    Species: Mouse
    Sample Types: Serum
  22. Strain-Specific Differences in House Dust Mite (Dermatophagoides farinae)-Induced Mouse Models of Allergic Rhinitis
    Authors: KI Lee, JS Bae, EH Kim, JH Kim, L Lyu, YJ Chung, JH Mo
    Clin Exp Otorhinolaryngol, 2020-05-15;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  23. Lactobacillus reuteri attenuated allergic inflammation induced by HDM in the mouse and modulated gut microbes
    Authors: L Li, Z Fang, X Liu, W Hu, W Lu, YK Lee, J Zhao, H Zhang, W Chen
    PLoS ONE, 2020-04-21;15(4):e0231865.
    Species: Mouse
    Sample Types: BALF
  24. Regnase-1 degradation is crucial for interleukin-33- and interleukin-25-mediated ILC2 activation
    Authors: K Matsushita, H Tanaka, K Yasuda, T Adachi, A Fukuoka, S Akasaki, A Koida, E Kuroda, S Akira, T Yoshimoto
    JCI Insight, 2020-02-27;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  25. Strongyloides venezuelensis-infection alters the profile of cytokines and liver inflammation in mice co-infected with Schistosoma mansoni
    Authors: MC de Rezende, JMP Moreira, LLM Fernandes, VF Rodrigues, D Negrão-Cor
    Cytokine, 2019-11-26;127(0):154931.
    Species: Mouse
    Sample Types: Tissue Homogenates
  26. Eosinophils Do Not Drive Acute Muscle Pathology in the mdx Mouse Model of Duchenne Muscular Dystrophy
    Authors: AC Sek, IN Moore, MG Smelkinson, K Pak, M Minai, R Smith, M Ma, CM Percopo, HF Rosenberg
    J. Immunol., 2019-05-29;0(0):.
    Species: Mouse
    Sample Types: Serum
  27. Conjugated bile acids attenuate allergen-induced airway inflammation and hyperresposiveness by inhibiting UPR transducers
    Authors: EM Nakada, NR Bhakta, BR Korwin-Mih, A Kumar, N Chamberlai, SR Bruno, DG Chapman, SM Hoffman, N Daphtary, M Aliyeva, CG Irvin, AE Dixon, PG Woodruff, S Amin, ME Poynter, DH Desai, V Anathy
    JCI Insight, 2019-05-02;4(9):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  28. Inhalation of the prodrug PI3K inhibitor CL27c improves lung function in asthma and fibrosis
    Authors: CC Campa, RL Silva, JP Margaria, T Pirali, MS Mattos, LR Kraemer, DC Reis, G Grosa, F Copperi, EM Dalmarco, RCP Lima-Júnio, S Aprile, V Sala, F Dal Bello, DS Prado, JC Alves-Filh, C Medana, GD Cassali, GC Tron, MM Teixeira, E Ciraolo, RC Russo, E Hirsch
    Nat Commun, 2018-12-12;9(1):5232.
    Species: Mouse
    Sample Types: Tissue Homogenates
  29. The Plant Proteinase Inhibitor CrataBL Plays a Role in Controlling Asthma Response in Mice
    Authors: ASS Bortolozzo, APD Rodrigues, FM Arantes-Co, BM Saraiva-Ro, FCR de Souza, TR Brüggemann, MV de Brito, RDS Ferreira, MTDS Correia, PMG Paiva, CM Prado, EA Leick, MLV Oliva, MA Martins, VC Ruiz-Schut, RF Righetti, IFLC Tibério
    Biomed Res Int, 2018-10-01;2018(0):9274817.
    Species: Mouse
    Sample Types: Tissue Homogenates
  30. Low dose of chlorine exposure exacerbates nasal and pulmonary allergic inflammation in mice
    Authors: IS de Genaro, FM de Almeida, DC Hizume-Kun, HT Moriya, RA Silva, JCG Cruz, RB Lopes, RF Righetti, R de Paula V, M Saiki, MA Martins, IFLC Tibério, FM Arantes-Co, BM Saraiva-Ro
    Sci Rep, 2018-08-22;8(1):12636.
    Species: Mouse
    Sample Types: Tissue Homogenates
  31. IL-33/ST2 axis mediates hyperplasia of intrarenal urothelium in obstructive renal injury
    Authors: WY Chen, JL Yang, YH Wu, LC Li, RF Li, YT Chang, LH Dai, WC Wang, YJ Chang
    Exp. Mol. Med., 2018-04-20;50(4):36.
    Species: Mouse
    Sample Types: Serum
  32. Probiotic treatment during neonatal age provides optimal protection against experimental asthma through the modulation of microbiota and T cells: Neonatal exposure to probiotics prevents asthma
    Authors: CFCG Nunes, JS Nogueira, FB Canto, PHO Vianna, BT Ciambarell, PMRE Silva, KR Miranda, LA Lobo, RMCP Domingues, M Busch, GC Atella, AM Vale, M Bellio, A Nóbrega, R Fucs
    Int. Immunol., 2018-04-03;0(0):.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Capture
  33. TPL-2 restricts Ccl24-dependent immunity to Heligmosomoides polygyrus
    Authors: Y Kannan, LJ Entwistle, VS Pelly, J Perez-Llor, AW Walker, SC Ley, MS Wilson
    PLoS Pathog., 2017-07-31;13(7):e1006536.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  34. IRF5 distinguishes severe asthma in humans and drives Th1 phenotype and airway hyperreactivity in mice
    Authors: TB Oriss, M Raundhal, C Morse, RE Huff, S Das, R Hannum, MC Gauthier, KL Scholl, K Chakrabort, SM Nouraie, SE Wenzel, P Ray, A Ray
    JCI Insight, 2017-05-18;2(10):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  35. Cationic CaMKII Inhibiting Nanoparticles Prevent Allergic Asthma
    Authors: AS Morris, SC Sebag, JD Paschke, A Wongrakpan, K Ebeid, ME Anderson, IM Grumbach, AK Salem
    Mol. Pharm., 2017-05-09;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  36. Host DNA released by NETosis promotes rhinovirus-induced type-2 allergic asthma exacerbation
    Authors: M Toussaint, DJ Jackson, D Swieboda, A Guedán, TD Tsouroukts, YM Ching, C Radermecke, H Makrinioti, J Aniscenko, MR Edwards, R Solari, F Farnir, V Papayannop, F Bureau, T Marichal, SL Johnston
    Nat. Med., 2017-05-01;23(6):681-691.
    Species: Mouse
    Sample Types: Tissue Homogenates
  37. Water-soluble chitosan inhibits nerve growth factor and attenuates allergic inflammation in mite allergen-induced allergic rhinitis
    Authors: PC Chen, MH Hsieh, P Wen-ShuoKu, HF Kao, CL Hsu, JY Wang
    J. Allergy Clin. Immunol., 2017-04-12;0(0):.
    Species: Mouse
    Sample Types: NLF
  38. Experimental atopic dermatitis depends on IL-33R signaling via MyD88 in dendritic cells
    Authors: C Li, I Maillet, C Mackowiak, C Viala, F Di Padova, M Li, D Togbe, V Quesniaux, Y Lai, B Ryffel
    Cell Death Dis, 2017-04-06;8(4):e2735.
    Species: Mouse
    Sample Types: Tissue Homogenates
  39. Mitochondrial CaMKII inhibition in airway epithelium protects against allergic asthma
    Authors: SC Sebag, OM Koval, JD Paschke, CJ Winters, OA Jaffer, R Dworski, FS Sutterwala, ME Anderson, IM Grumbach
    JCI Insight, 2017-02-09;2(3):e88297.
    Species: Mouse
    Sample Types: Tissue Homogenates
  40. Hp91 immunoadjuvant: An HMGB1-derived peptide for development of therapeutic HPV vaccines
    Authors: Somayeh Talebi
    Biomed. Pharmacother, 2016-12-05;85(0):148-154.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  41. Acid-Sensing Ion Channel 1a Contributes to Airway Hyperreactivity in Mice
    PLoS ONE, 2016-11-07;11(11):e0166089.
    Species: Mouse
    Sample Types: BALF
  42. The soluble guanylyl cyclase activator BAY 60-2770 inhibits murine allergic airways inflammation and human eosinophil chemotaxis
    Authors: Edson Antunes
    Pulm Pharmacol Ther, 2016-11-03;0(0):.
    Species: Mouse
    Sample Types: BALF
  43. DUOX1 mediates persistent epithelial EGFR activation, mucous cell metaplasia, and airway remodeling during allergic asthma
    JCI Insight, 2016-11-03;1(18):e88811.
    Species: Mouse
    Sample Types: BALF
  44. IL-27 Is Essential for Suppression of Experimental Allergic Asthma by the TLR7/8 Agonist R848 (Resiquimod)
    Authors: Gesine Hansen
    J. Immunol., 2016-10-31;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
  45. Allergen-Induced IL-6 Regulates IL-9/IL-17A Balance in CD4+ T Cells in Allergic Airway Inflammation
    J Immunol, 2016-08-29;197(7):2653-64.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  46. Impact of Histone H1 on the Progression of Allergic Rhinitis and Its Suppression by Neutralizing Antibody in Mice
    Authors: T Nakano, R Kamei, T Fujimura, Y Takaoka, A Hori, CY Lai, KC Chiang, Y Shimada, N Ohmori, T Goto, K Ono, CL Chen, S Goto, S Kawamoto
    PLoS ONE, 2016-04-18;11(4):e0153630.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  47. IL-22 Restrains Tapeworm-Mediated Protection against Experimental Colitis via Regulation of IL-25 Expression
    Authors: JL Reyes, MR Fernando, F Lopes, G Leung, NL Mancini, CE Matisz, A Wang, DM McKay
    PLoS Pathog, 2016-04-07;12(4):e1005481.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  48. Hydroxypropyl-beta-cyclodextrin spikes local inflammation that induces Th2 cell and T follicular helper cell responses to the coadministered antigen.
    Authors: Onishi M, Ozasa K, Kobiyama K, Ohata K, Kitano M, Taniguchi K, Homma T, Kobayashi M, Sato A, Katakai Y, Yasutomi Y, Wijaya E, Igarashi Y, Nakatsu N, Ise W, Inoue T, Yamada H, Vandenbon A, Standley D, Kurosaki T, Coban C, Aoshi T, Kuroda E, Ishii K
    J Immunol, 2015-02-13;194(6):2673-82.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  49. SLy2 controls the antibody response to pneumococcal vaccine through an IL-5Ralpha-dependent mechanism in B-1 cells.
    Authors: Schmitt F, Schall D, Bucher K, Schindler T, Hector A, Biedermann T, Zemlin M, Hartl D, Beer-Hammer S
    Eur J Immunol, 2014-11-13;45(1):60-70.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  50. Lifetime-dependent effects of bisphenol A on asthma development in an experimental mouse model.
    Authors: Petzold, Susanne, Averbeck, Marco, Simon, Jan C, Lehmann, Irina, Polte, Tobias
    PLoS ONE, 2014-06-20;9(6):e100468.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  51. Sm10.3, a member of the micro-exon gene 4 (MEG-4) family, induces erythrocyte agglutination in vitro and partially protects vaccinated mice against Schistosoma mansoni infection.
    Authors: Martins V, Morais S, Pinheiro C, Assis N, Figueiredo B, Ricci N, Alves-Silva J, Caliari M, Oliveira S
    PLoS Negl Trop Dis, 2014-03-20;8(3):e2750.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  52. Subcomponent vaccine based on CTA1-DD adjuvant with incorporated UreB class II peptides stimulates protective Helicobacter pylori immunity.
    Authors: Nedrud J, Bagheri N, Schon K, Xin W, Bergroth H, Eliasson D, Lycke N
    PLoS ONE, 2013-12-31;8(12):e83321.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  53. MyD88 signaling regulates both host defense and immunopathogenesis during pneumocystis infection.
    Authors: Bello-Irizarry S, Wang J, Johnston C, Gigliotti F, Wright T
    J Immunol, 2013-11-29;192(1):282-92.
    Species: Mouse
    Sample Types: BALF
  54. Alveolar Macrophages Play a Key Role in Cockroach-Induced Allergic Inflammation via TNF-alpha Pathway.
    Authors: Kim J, Sohn J, Choi J, Lee J, Hong C, Lee J, Park J
    PLoS ONE, 2012-10-19;7(10):e47971.
    Species: Mouse
    Sample Types: Tissue Homogenates
  55. Schistosome-derived omega-1 drives Th2 polarization by suppressing protein synthesis following internalization by the mannose receptor.
    J. Exp. Med., 2012-09-10;209(10):1753-67, S1.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  56. Control of allergen-induced inflammation and hyperresponsiveness by the metalloproteinase ADAMTS-12.
    Authors: Paulissen G, El Hour M, Rocks N, Gueders M, Bureau F, Foidart J, Lopez-Otin C, Noel A, Cataldo D
    J Immunol, 2012-09-07;189(8):4135-43.
    Species: Mouse
    Sample Types: Tissue Homogenates
  57. IL-33-responsive lineage- CD25+ CD44(hi) lymphoid cells mediate innate type 2 immunity and allergic inflammation in the lungs.
    Authors: Bartemes KR, Iijima K, Kobayashi T, Kephart GM, McKenzie AN, Kita H
    J. Immunol., 2011-12-23;188(3):1503-13.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  58. Role of Toll interleukin-1 receptor (IL-1R) 8, a negative regulator of IL-1R/Toll-like receptor signaling, in resistance to acute Pseudomonas aeruginosa lung infection.
    Authors: Veliz Rodriguez T, Moalli F, Polentarutti N, Paroni M, Bonavita E, Anselmo A, Nebuloni M, Mantero S, Jaillon S, Bragonzi A, Mantovani A, Riva F, Garlanda C
    Infect. Immun., 2011-10-24;80(1):100-9.
    Species: Mouse
    Sample Types: Serum
  59. T cell-derived Act1 is necessary for IL-25-mediated Th2 responses and allergic airway inflammation.
    Authors: Swaidani S, Bulek K, Kang Z
    J. Immunol., 2011-08-19;187(6):3155-64.
    Species: Mouse
    Sample Types: BALF
  60. Impaired basophil induction leads to an age-dependent innate defect in type 2 immunity during helminth infection in mice.
    Authors: Nel HJ, Hams E, Saunders SP
    J. Immunol., 2011-03-11;186(8):4631-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  61. Alpha-Galactosylceramide-induced airway eosinophilia is mediated through the activation of NKT cells.
    Authors: Chuang YH, Wang TC, Jen HY, Yu AL, Chiang BL
    J. Immunol., 2011-03-07;186(8):4687-92.
    Species: Mouse
    Sample Types: BALF
  62. A critical role for C5L2 in the pathogenesis of experimental allergic asthma.
    Authors: Zhang X, Schmudde I, Laumonnier Y, Pandey MK, Clark JR, Konig P, Gerard NP, Gerard C, Wills-Karp M, Kohl J
    J. Immunol., 2010-10-25;185(11):6741-52.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  63. Th17 cells are the dominant T cell subtype primed by Shigella flexneri mediating protective immunity.
    Authors: Sellge G, Magalhaes JG, Konradt C, Fritz JH, Salgado-Pabon W, Eberl G, Bandeira A, Di Santo JP, Sansonetti PJ, Phalipon A
    J. Immunol., 2010-01-20;184(4):2076-85.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  64. Effects of umbelliferone in a murine model of allergic airway inflammation.
    Authors: Vasconcelos JF, Teixeira MM, Barbosa-Filho JM, Agra MF, Nunes XP, Giulietti AM, Ribeiro-Dos-Santos R, Soares MB
    Eur. J. Pharmacol., 2009-03-14;609(1):126-31.
    Species: Mouse
    Sample Types: BALF
  65. A chlamydial type III-secreted effector protein (Tarp) is predominantly recognized by antibodies from humans infected with Chlamydia trachomatis and induces protective immunity against upper genital tract pathologies in mice.
    Authors: Wang J, Chen L, Chen F, Zhang X, Zhang Y, Baseman J, Perdue S, Yeh IT, Shain R, Holland M, Bailey R, Mabey D, Yu P, Zhong G
    Vaccine, 2009-03-10;27(22):2967-80.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  66. Mucosal immunity in mice induced by orally administered transgenic rice.
    Authors: Zhang X, Yuan Z, Duan Q, Zhu H, Yu H, Wang Q
    Vaccine, 2009-01-13;27(10):1596-600.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  67. Identification of functional roles for both IL-17RB and IL-17RA in mediating IL-25-induced activities.
    Authors: Rickel EA, Siegel LA, Yoon BR, Rottman JB, Kugler DG, Swart DA, Anders PM, Tocker JE, Comeau MR, Budelsky AL
    J. Immunol., 2008-09-15;181(6):4299-310.
    Species: Mouse
    Sample Types: BALF
  68. Adenosine A2A receptor activation inhibits T helper 1 and T helper 2 cell development and effector function.
    Authors: Csoka B, Himer L, Selmeczy Z, Vizi ES, Pacher P, Ledent C, Deitch EA, Spolarics Z, Nemeth ZH, Hasko G
    FASEB J., 2008-07-14;22(10):3491-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  69. Distinct roles for IL-13 and IL-4 via IL-13 receptor alpha1 and the type II IL-4 receptor in asthma pathogenesis.
    Authors: Munitz A, Brandt EB, Mingler M, Finkelman FD, Rothenberg ME
    Proc. Natl. Acad. Sci. U.S.A., 2008-05-14;105(20):7240-5.
    Species: Mouse
    Sample Types: BALF
  70. Transforming growth factor-beta regulates house dust mite-induced allergic airway inflammation but not airway remodeling.
    Authors: Fattouh R, Midence NG, Arias K, Johnson JR, Walker TD, Goncharova S, Souza KP, Gregory RC, Lonning S, Gauldie J, Jordana M
    Am. J. Respir. Crit. Care Med., 2008-01-03;177(6):593-603.
    Species: Mouse
    Sample Types: BALF
  71. Lipoxin A4 stable analogs reduce allergic airway responses via mechanisms distinct from CysLT1 receptor antagonism.
    Authors: Levy BD, Lukacs NW, Berlin AA, Schmidt B, Guilford WJ, Serhan CN, Parkinson JF
    FASEB J., 2007-07-11;21(14):3877-84.
    Species: Mouse
    Sample Types: Tissue Homogenates
  72. Coexposure to environmental tobacco smoke increases levels of allergen-induced airway remodeling in mice.
    Authors: Min MG, Song DJ, Miller M, Cho JY, McElwain S, Ferguson P, Broide DH
    J. Immunol., 2007-04-15;178(8):5321-8.
    Species: Mouse
    Sample Types: BALF
  73. Toll-like receptor-2-mediated C-C chemokine receptor 3 and eotaxin-driven eosinophil influx induced by Mycobacterium bovis BCG pleurisy.
    Authors: D'Avila H, Almeida PE, Roque NR, Castro-Faria-Neto HC, Bozza PT
    Infect. Immun., 2006-12-11;75(3):1507-11.
    Species: Mouse
    Sample Types: Pleural Effusion
  74. Interleukin-17 is a negative regulator of established allergic asthma.
    Authors: Schnyder-Candrian S, Togbe D, Couillin I, Mercier I, Brombacher F, Quesniaux V, Fossiez F, Ryffel B, Schnyder B
    J. Exp. Med., 2006-11-13;203(12):2715-25.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  75. Induced trefoil factor family 1 expression by trans-differentiating Clara cells in a murine asthma model.
    Authors: Kouznetsova I, Chwieralski CE, Balder R, Hinz M, Braun A, Krug N, Hoffmann W
    Am. J. Respir. Cell Mol. Biol., 2006-09-21;36(3):286-95.
    Species: Mouse
    Sample Types: BALF
  76. Pulmonary distribution, regulation, and functional role of Trk receptors in a murine model of asthma.
    Authors: Nassenstein C, Dawbarn D, Pollock K, Allen SJ, Erpenbeck VJ, Spies E, Krug N, Braun A
    J. Allergy Clin. Immunol., 2006-07-03;118(3):597-605.
    Species: Mouse
    Sample Types: BALF
  77. Exogenous IL-12 suppresses experimental autoimmune encephalomyelitis (EAE) by tuning IL-10 and IL-5 levels in an IFN-gamma-dependent way.
    Authors: Berghmans N, Dillen C, Heremans H
    J. Neuroimmunol., 2006-06-09;176(1):63-75.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  78. Hierarchical suppression of asthma-like responses by mucosal tolerance.
    Authors: Keller AC, Mucida D, Gomes E, Faquim-Mauro E, Faria AM, Rodriguez D, Russo M
    J. Allergy Clin. Immunol., 2006-02-01;117(2):283-90.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  79. Comparison of immunogenicities of recombinant Plasmodium vivax merozoite surface protein 1 19- and 42-kiloDalton fragments expressed in Escherichia coli.
    Authors: Sachdeva S, Ahmad G, Malhotra P, Mukherjee P, Chauhan VS
    Infect. Immun., 2004-10-01;72(10):5775-82.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  80. Transcutaneous immunization with cholera toxin B subunit adjuvant suppresses IgE antibody responses via selective induction of Th1 immune responses.
    Authors: Anjuere F, George-Chandy A, Audant F, Rousseau D, Holmgren J, Czerkinsky C
    J. Immunol., 2003-02-01;170(3):1586-92.
    Species: Mouse
    Sample Types: Cell Culture Supernates

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Mouse IL-5 DuoSet ELISA
By Anonymous on 01/25/2020
Sample Tested: Splenic T cells