Mouse IL-5 R alpha/CD125 Biotinylated Antibody

Catalog #: BAF553 Datasheet / COA / SDS

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BAF553

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Mouse IL-5 R alpha/CD125 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL‑5 R alpha /CD125 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) IL-5 R alpha is observed and less than 1% cross-reactivity with recombinant mouse (rm) IL-4 R, rhIL-9 R, rhIL-13 R alpha 1, and rmIL-13 R alpha 2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL‑5 R alpha /CD125
Asp18-His339
Accession # P21183

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse IL‑5 R alpha /CD125

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-5 R alpha/CD125

Interleukin‑5 Receptor alpha (IL‑5 R alpha ), also known as CD125, is a 60 kDa hematopoietin receptor that plays a dominant role in eosinophil biology (1‑3). Mature mouse IL‑5 R alpha consists of a 322 amino acid (aa) extracellular domain (ECD) with a WSxWS motif and a four cysteine motif, a 22 aa transmembrane segment, and a 54 aa cytoplasmic domain (4). Within the ECD, mouse IL‑5 R alpha shares 71% and 86% aa sequence identity with human and rat IL‑5 R alpha, respectively. Alternate splicing of mouse IL‑5 R alpha generates soluble secreted forms which function as IL‑5 antagonists (4, 5). The high affinity receptor for IL‑5 is a complex that consists of the ligand binding IL‑5 R alpha and the transmembrane common beta chain ( beta c/CD131) which is shared with the receptor complexes for IL‑3 and GM‑CSF (6). IL‑5 R alpha binds IL‑5 at low affinity and then associates with preformed beta c oligomers to form the signaling‑competent receptor complex (7). IL‑5 stimulation of CD34⁺ hematopoietic progenitor cells induces the up‑regulation of transmembrane IL‑5 R alpha followed by eosinophilic differentiation and activation (8‑10). IL‑5 R alpha also promotes the differentiation of basophils and B cells (11, 12). Exposure of mature eosinophils to IL‑5 attenuates their IL‑5 responsiveness by inducing the down‑regulation of surface IL‑5 R alpha and increased production of soluble IL‑5 R alpha (13, 14). Elevated production of IL‑5 at sites of allergic inflammation induces eosinophilia and exacerbation of immune cell infiltration, tissue damage, and remodeling (2, 3).

Long Name

Interleukin 5 Receptor alpha

Entrez Gene IDs

3568 (Human); 16192 (Mouse)

Alternate Names

CD125 antigen; CD125; CDw125CD125; HSIL5R3; IL-5 R alpha; IL-5 receptor subunit alpha; IL5R alpha; IL-5R subunit alpha; IL5R; IL5RA; IL-5Ra; IL-5R-alpha; interleukin 5 receptor type 3; interleukin 5 receptor, alpha; interleukin-5 receptor alpha chain; interleukin-5 receptor alpha subunit; interleukin-5 receptor subunit alpha; MGC26560