Mouse IL-5 R alpha /CD125 Antibody Summary
Asp18-His339
Accession # P21183
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-5 R alpha/CD125
Interleukin‑5 Receptor alpha (IL‑5 R alpha ), also known as CD125, is a 60 kDa hematopoietin receptor that plays a dominant role in eosinophil biology (1‑3). Mature mouse IL‑5 R alpha consists of a 322 amino acid (aa) extracellular domain (ECD) with a WSxWS motif and a four cysteine motif, a 22 aa transmembrane segment, and a 54 aa cytoplasmic domain (4). Within the ECD, mouse IL‑5 R alpha shares 71% and 86% aa sequence identity with human and rat IL‑5 R alpha, respectively. Alternate splicing of mouse IL‑5 R alpha generates soluble secreted forms which function as IL‑5 antagonists (4, 5). The high affinity receptor for IL‑5 is a complex that consists of the ligand binding IL‑5 R alpha and the transmembrane common beta chain ( beta c/CD131) which is shared with the receptor complexes for IL‑3 and GM‑CSF (6). IL‑5 R alpha binds IL‑5 at low affinity and then associates with preformed beta c oligomers to form the signaling‑competent receptor complex (7). IL‑5 stimulation of CD34+ hematopoietic progenitor cells induces the up‑regulation of transmembrane IL‑5 R alpha followed by eosinophilic differentiation and activation (8‑10). IL‑5 R alpha also promotes the differentiation of basophils and B cells (11, 12). Exposure of mature eosinophils to IL‑5 attenuates their IL‑5 responsiveness by inducing the down‑regulation of surface IL‑5 R alpha and increased production of soluble IL‑5 R alpha (13, 14). Elevated production of IL‑5 at sites of allergic inflammation induces eosinophilia and exacerbation of immune cell infiltration, tissue damage, and remodeling (2, 3).
- Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
- Rothenberg, M.E. and S.P. Hogan (2005) Annu. Rev. Immunol. 24:147.
- Elsas, X.P. and M.I.G. Elsas (2007) Curr. Med. Chem. 14:1925.
- Takaki, S. et al. (1990) EMBO J. 9:4367.
- Cameron, L. et al. (2000) J. Immunol. 164:1538.
- Tavernier, J. et al. (1991) Cell 66:1175.
- Zaks-Zilberman, M. et al. (2008) J. Biol. Chem. 283:13398.
- Tavernier, J. et al. (2000) Blood 95:1600.
- Clutterbuck, E.J. et al. (1989) Blood 73:1504.
- Lopez, A.F. et al. (1988) J. Exp. Med. 167:219.
- Denburg, J.A. et al. (1991) Blood 77:1462.
- Hasbold, J. et al. (2004) Nat. Immunol. 5:55.
- Gregory, B. et al. (2003) J. Immunol. 170:5359.
- Liu, L.Y. et al. (2002) J. Immunol. 169:6459.
Product Datasheets
Citation for Mouse IL-5 R alpha /CD125 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Defective eosinophil hematopoiesis ex vivo in inbred Rocky Mountain White (IRW) mice.
Authors: Dyer KD, Garcia-Crespo KE, Percopo CM, Bowen AB, Ito T, Peterson KE, Gilfillan AM, Rosenberg HF
J. Leukoc. Biol., 2011-08-30;90(6):1101-9.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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