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Mouse Leptin/OB Antibody

Catalog #: AF498
26 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF498
AF498-SP
Leptin/OB in Mouse Brain.
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Citations (26)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Leptin/OB Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Leptin/OB in ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 µg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Leptin/OB (Catalog # 498-OB)
Immunohistochemistry
5-15 µg/mL
See below

Mouse Leptin/OB Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse Leptin/OB Biotinylated Antibody (Catalog # BAF498)

Standard: Recombinant Mouse Leptin Protein, CF (Catalog # 498-OB)

Neutralization
Measured by its ability to neutralize Leptin/OB-induced proliferation in the BaF3 mouse pro‑B cell line transfected with human Leptin R. The Neutralization Dose (ND50) is typically 0.05-0.3 µg/mL in the presence of 2.5 ng/mL Recombinant Mouse Leptin/OB.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Leptin/OB antibody in Mouse Brain by Immunohistochemistry (IHC-Fr). View Larger

Leptin/OB in Mouse Brain. Leptin/OB was detected in perfusion fixed frozen sections of mouse brain using Mouse Leptin/OB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF498) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Leptin/OB

Leptin is a protein product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of Leptin have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse, and rat cells. Mouse Leptin shares approximately 96% and 84% sequence identity with the rat and human protein, respectively. Mouse Leptin cDNA encodes a 167 amino acid residue protein with a 21 amino acid residue signal sequence that is cleaved to yield the 146 amino acid residue mature protein. The expression of Leptin mRNA has been shown to be restricted to adipose tissue.

A high-affinity receptor for Leptin (OB-R) with homology to gp130 and the G-CSF receptor has been cloned. OB-R mRNA has been shown to be expressed in the choroid plexus and in the hypothalamus. OB-R has also been identified as an isoform of B219, a sequence that is expressed in at least four isoforms in very primitive hematopoietic cell populations and in a variety of lymphohematopoietic cell lines (1-3). The possible roles of Leptin in body weight regulation, hematopoiesis and reproduction are being investigated.

References
  1. Considine, R. and J. Caro (1996) Clinical Chemistry 42:843.
  2. Tartaglia, L.A. et al. (1995) Cell 83:1263.
  3. Cioffi, J.A. et al. (1996) Nature Medicine 2:585.
Entrez Gene IDs
3952 (Human); 16846 (Mouse); 25608 (Rat)
Alternate Names
FLJ94114; LEP; leptin (murine obesity homolog); leptin (obesity homolog, mouse); Leptin; OB; Obese protein; obese, mouse, homolog of; Obesity factor; OBOBS

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Citations for Mouse Leptin/OB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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  1. Oncolytic Viruses Engineered to Enforce Leptin Expression Reprogram Tumor-Infiltrating T Cell Metabolism and Promote Tumor Clearance
    Authors: Dayana B. Rivadeneira, Kristin DePeaux, Yiyang Wang, Aditi Kulkarni, Tracy Tabib, Ashley V. Menk et al.
    Immunity
  2. Correction--JAK/STAT3-Regulated Fatty Acid beta -Oxidation Is Critical for Breast Cancer Stem Cell Self-Renewal and Chemoresistance
    Authors: Tianyi Wang, Johannes Francois Fahrmann, Heehyoung Lee, Yi-Jia Li, Satyendra C. Tripathi, Chanyu Yue et al.
    Cell Metabolism
  3. Extra-adrenal glucocorticoids contribute to the postprandial increase of circulating leptin in mice
    Authors: Yuka Tomabechi, Takeshi Tsuruta, Shinichi Saito, Martin Wabitsch, Kei Sonoyama
    Journal of Cell Communication and Signaling
  4. Secreted frizzled‑related protein 4 promotes brown adipocyte differentiation
    Authors: Hua Guan, Huiyuan Zheng, Jin Zhang, Aoqi Xiang, Yongxin Li, Huadong Zheng et al.
    Experimental and Therapeutic Medicine
  5. Chemerin influences blood lipid of aged male mice under high fat diet and exercise states through regulating the distribution and browning of white adipose tissue
    Authors: Qu, J;Fu, S;Yin, L;Zhang, Q;Wang, X;
    Cytokine
    Species: Mouse, Transgenic Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. Deletion of endothelial leptin receptors in mice promotes diet-induced obesity
    Authors: Gogiraju, R;Witzler, C;Shahneh, F;Hubert, A;Renner, L;Bochenek, ML;Zifkos, K;Becker, C;Thati, M;Sch�fer, K;
    Scientific reports
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Obesity-associated leptin promotes chemoresistance in colorectal cancer through YAP-dependent AXL upregulation
    Authors: YC Chen, CY Chien, CC Hsu, CH Lee, YT Chou, SG Shiah, SY Liu, CY Yen, AC Hsieh, M Wabitsch, YS Shieh
    American journal of cancer research, 2021-09-15;11(9):4220-4240.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  8. Ectopic Leptin Production by Intraocular Pancreatic Islet Organoids Ameliorates the Metabolic Phenotype of ob/ob Mice
    Authors: B Leibiger, T Moede, I Valladolid, M Paschen, M Visa, IB Leibiger, PO Berggren
    Metabolites, 2021-06-14;11(6):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Localized Antileptin Therapy Prevents Aortic Root Dilatation and Preserves Left Ventricular Systolic Function in a Murine Model of Marfan Syndrome
    Authors: S Fisch, N Bachner-Hi, O Ertracht, L Guo, Y Arad, D Ben-Zvi, R Liao, J Schneiderm
    J Am Heart Assoc, 2020-05-07;9(10):e014761.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Exercise reduces inflammatory cell production and cardiovascular inflammation via instruction of hematopoietic progenitor cells
    Authors: V Frodermann, D Rohde, G Courties, N Severe, MJ Schloss, H Amatullah, CS McAlpine, S Cremer, FF Hoyer, F Ji, ID van Koever, F Herisson, L Honold, GS Masson, S Zhang, J Grune, Y Iwamoto, SP Schmidt, GR Wojtkiewic, IH Lee, K Gustafsson, G Pasterkamp, SCA de Jager, RI Sadreyev, J MacFadyen, P Libby, P Ridker, DT Scadden, K Naxerova, KL Jeffrey, FK Swirski, M Nahrendorf
    Nat. Med., 2019-11-07;25(11):1761-1771.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  11. Regulation of the bi-directional cross-talk between ovarian cancer cells and adipocytes by SPARC
    Authors: B John, C Naczki, C Patel, A Ghoneum, S Qasem, Z Salih, N Said
    Oncogene, 2019-02-14;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  12. Elevated leptin disrupts epithelial polarity and promotes premalignant alterations in the mammary gland
    Authors: I Tenvooren, MZ Jenks, H Rashid, KL Cook, JK Muhlemann, C Sistrunk, J Holmes, K Wang, K Bonin, K Hodges, HW Lo, A Shaikh, IG Camarillo, SA Lelièvre, V Seewaldt, PA Vidi
    Oncogene, 2019-01-22;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Involvement of leptin signaling in the development of cannabinoid CB2 receptor-dependent mirror image pain
    Authors: C Nozaki, E Nent, A Bilkei-Gor, A Zimmer
    Sci Rep, 2018-07-17;8(1):10827.
    Species: Mouse
    Sample Types: In Vivo, Whole Tissue
    Applications: IHC, Neutralization
  14. Leptin/Osteopontin Axis Regulated Type 2T Helper Cell Response in Allergic Rhinitis with Obesity
    Authors: Q Zeng, X Luo, M Han, W Liu, H Li
    EBioMedicine, 2018-06-07;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  15. Data on the expression of leptin and leptin receptor in the dorsal root ganglion and spinal cord after preganglionic cervical root avulsion
    Authors: KT Chang, YL Lin, CT Lin, MJ Tsai, WC Huang, YH Shih, YY Lee, H Cheng, MC Huang
    Data Brief, 2017-10-07;15(0):567-572.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  16. Leptin sustains spontaneous remyelination in the adult central nervous system
    Authors: K Matoba, R Muramatsu, T Yamashita
    Sci Rep, 2017-01-16;7(0):40397.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  17. Rapid linkage of innate immunological signals to adaptive immunity by the brain-fat axis.
    Authors: Kim, Min Soo, Yan, Jingqi, Wu, Wenhe, Zhang, Guo, Zhang, Yalin, Cai, Dongshen
    Nat Immunol, 2015-04-06;16(5):525-33.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  18. Hypothalamic tanycytes are an ERK-gated conduit for leptin into the brain.
    Authors: Balland E, Dam J, Langlet F, Caron E, Steculorum S, Messina A, Rasika S, Falluel-Morel A, Anouar Y, Dehouck B, Trinquet E, Jockers R, Bouret S, Prevot V
    Cell Metab, 2014-02-04;19(2):293-301.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  19. Leptin-dependent and leptin-independent paracrine effects of perivascular adipose tissue on neointima formation.
    Authors: Schroeter M, Eschholz N, Herzberg S, Jerchel I, Leifheit-Nestler M, Czepluch F, Chalikias G, Konstantinides S, Schafer K
    Arterioscler Thromb Vasc Biol, 2013-03-21;33(5):980-7.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. Leptin derived from adipocytes in injured peripheral nerves facilitates development of neuropathic pain via macrophage stimulation.
    Authors: Maeda T, Kiguchi N, Kobayashi Y, Ikuta T, Ozaki M, Kishioka S
    Proc. Natl. Acad. Sci. U.S.A., 2009-07-20;106(31):13076-81.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  21. Interferon-alpha suppresses leptin levels: studies in interferon-alpha treated patients with hepatitis C virus infection and murine adipocytes.
    Authors: Kaser S, Kaser A, Vogel W, Patsch JR, Tilg H
    Eur. Cytokine Netw., 2002-04-01;13(2):225-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  22. Leptin Mediates the Increase in Blood Pressure Associated with Obesity
    Authors: Stephanie E. Simonds, Jack T. Pryor, Eric Ravussin, Frank L. Greenway, Ralph Dileone, Andrew M. Allen et al.
    Cell
  23. Effects of Enriched Environment on COX-2, Leptin and Eicosanoids in a Mouse Model of Breast Cancer
    Authors: Rachida Nachat-Kappes, Alexandre Pinel, Kristell Combe, Bruno Lamas, Marie-Chantal Farges, Adrien Rossary et al.
    PLoS ONE
  24. Pluronic modified leptin with increased systemic circulation, brain uptake and efficacy for treatment of obesity☆
    Authors: Xiang Yi, Dongfen Yuan, Susan A. Farr, William A. Banks, Chi-Duen Poon, Alexander V. Kabanov
    Journal of Controlled Release
  25. STAT3 Activation-Induced Fatty Acid Oxidation in CD8+ T Effector Cells Is Critical for Obesity-Promoted Breast Tumor Growth
    Authors: Chunyan Zhang, Chanyu Yue, Andreas Herrmann, Jieun Song, Colt Egelston, Tianyi Wang et al.
    Cell Metabolism
  26. Personalized integrative oncology: targeted approaches for optimal outcomes: the 11th International Conference of the Society for Integrative Oncology
    Authors: Richard T Lee, Peiying Yang, Heather Greenlee, Susan Bauer-Wu, Lynda G. Balneaves, Suzanna Zick
    Integrative Cancer Therapies

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