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Mouse M-CSF Antibody

Catalog #: MAB4161
7 Citations Datasheet / COA / SDS
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MAB4161
MAB4161-SP
Cell Proliferation Induced by M‑CSF and Neutralization by Mouse M‑CSF Antibody.
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Citations (7)
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Mouse M-CSF Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse M-CSF in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human M-CSF or recombinant mouse SCF is observed.
Source
Monoclonal Rat IgG2B Clone # 131614
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse M-CSF
Lys33-Glu262 (predicted)
Accession # P07141
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse M-CSF (Catalog # 416-ML)
Neutralization
Measured by its ability to neutralize M‑CSF-induced proliferation in the M‑NFS‑60 mouse myelogenous luekemia lymphoblast cell line. Halenbeck, R. et al. (1989) Biotechnology 7:710. The Neutralization Dose (ND50) is typically 3-12 µg/mL in the presence of 10 ng/mL Recombinant Mouse M‑CSF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by M‑CSF and Neutralization by Mouse M‑CSF Antibody. View Larger

Cell Proliferation Induced by M‑CSF and Neutralization by Mouse M‑CSF Antibody. Recombinant Mouse M-CSF (Catalog # 416-ML) stimulates proliferation in the M-NFS-60 mouse myelogenous luekemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse M-CSF (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse M-CSF Monoclonal Antibody (Catalog # MAB4161). The ND50 is typically 3-12 µg/mL.

Immunocytochemistry/ Immunofluorescence Detection of Mouse M-CSF by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse M-CSF by Immunocytochemistry/Immunofluorescence Injury-induced SSEA-1 positive cells were regulated by topical application of M-CSF or M-CSF neutralizing antibody.Skin wounds in mice were treated by either PBS control (top panels) or topical application of recombinant M-CSF protein (middle panels) or topical application of M-CSF neutralizing antibody for 6 days. This figure showed a representative skin section from 6 wounds in each group, stained with SSEA-1 (red color). DAPI (blue) was used for cell nucleus staining. Scale bars, 100 μm. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep28979), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse M-CSF by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse M-CSF by Immunocytochemistry/Immunofluorescence SSEA-1 positive cells are also SSEA-3 positive and express M-CSF receptor.(A) Injured skin sections were stained with SSEA-1 (red color) and SSEA-3 (green color) antibodies. IgG was used for a negative staining control. DAPI (blue) was used for cell nucleus staining. Scale bars, 50 μm. (B) Injured skin sections were stained with SSEA-1 (green color) and M-CSF receptor (red color) antibodies. DAPI (blue) was used for cell nucleus staining. Scale bars, 50 μm. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep28979), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: M-CSF

M-CSF, also known as CSF-1, is a four-alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation (1-3). M-CSF is also essential for the survival and proliferation of osteoclast progenitors (1, 4). M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis (2, 3). M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta (5). Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells, and activated endothelial cells (1-5). The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur (3-9). Full length mouse M-CSF transcripts encode a 520 amino acid (aa) type I transmembrane (TM) protein with a 462 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O-glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen (8). Shorter transcripts encode M-CSF that lacks cleavage and PG sites and produces an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer (7). Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor (10, 11). The first 229 aa of mature mouse M-CSF shares 87%, 83%, 82%, and 81% aa identity with corresponding regions of rat, dog, cow, and human M-CSF, respectively (12, 13). Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

References
  1. Pixley, F.J. and E.R. Stanley (2004) Trends Cell Biol. 14:628.
  2. Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.
  3. Fixe, P. and V. Praloran (1997) Eur. Cytokine Netw. 8:125.
  4. Ryan, G.R. et al. (2001) Blood 98:74.
  5. Makrigiannakis, A. et al. (2006) Trends Endocrinol. Metab. 17:178.
  6. Nandi, S. et al. (2006) Blood 107:786.
  7. Rettenmier, C.W. and M.F. Roussel (1988) Mol. Cell Biol. 8:5026.
  8. Suzu, S. et al. (1992) J. Biol. Chem. 267:16812.
  9. Manos, M.M. (1988) Mol. Cell. Biol. 8:5035.
  10. Koths, K. (1997) Mol. Reprod. Dev. 46:31.
  11. Jang, M-H. et al. (2006) J. Immunol. 177:4055.
  12. DeLamarter, J.F. et al. (1987) Nucleic Acids Res. 15:2389.
  13. Ladner, M.B. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6706.
Long Name
Macrophage Colony Stimulating Factor
Entrez Gene IDs
1435 (Human); 12977 (Mouse)
Alternate Names
colony stimulating factor 1 (macrophage); CSF1; CSF-1; Lanimostim; macrophage colony stimulating factor; macrophage colony-stimulating factor 1; MCSF; M-CSF; MCSFlanimostim; MGC31930

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Citations for Mouse M-CSF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Enteric Glia Modulate Macrophage Phenotype and Visceral Sensitivity following Inflammation
    Authors: V Grubiši?, JL McClain, DE Fried, I Grants, P Rajasekhar, E Csizmadia, OA Ajijola, RE Watson, DP Poole, SC Robson, FL Christofi, BD Gulbransen
    Cell Rep, 2020-09-08;32(10):108100.
    Species: Mouse
    Sample Types: In situ
    Applications: Activation
  2. Bone-derived Nestin-positive mesenchymal stem cells improve cardiac function via recruiting cardiac endothelial cells after myocardial infarction
    Authors: D Lu, Y Liao, SH Zhu, QC Chen, DM Xie, JJ Liao, X Feng, MH Jiang, W He
    Stem Cell Res Ther, 2019-04-27;10(1):127.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Western Blot
  3. Interleukin-18 Amplifies Macrophage Polarization and Morphological Alteration, Leading to Excessive Angiogenesis
    Authors: T Kobori, S Hamasaki, A Kitaura, Y Yamazaki, T Nishinaka, A Niwa, S Nakao, H Wake, S Mori, T Yoshino, M Nishibori, H Takahashi
    Front Immunol, 2018-03-06;9(0):334.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: Neutralization, Western Blot
  4. An inflammatory gene signature distinguishes neurofibroma Schwann cells and macrophages from cells in the normal peripheral nervous system
    Authors: K Choi, K Komurov, JS Fletcher, E Jousma, JA Cancelas, J Wu, N Ratner
    Sci Rep, 2017-03-03;7(0):43315.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  5. CSF-1 receptor-mediated differentiation of a new type of monocytic cell with B cell-stimulating activity: its selective dependence on IL-34.
    Authors: Yamane F, Nishikawa Y, Matsui K, Asakura M, Iwasaki E, Watanabe K, Tanimoto H, Sano H, Fujiwara Y, Stanley E, Kanayama N, Mabbott N, Magari M, Ohmori H
    J Leukoc Biol, 2013-09-19;95(1):19-31.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Colony-stimulating factor 1 receptor (CSF1R) signaling in injured neurons facilitates protection and survival.
    Authors: Luo, Jian, Elwood, Fiona, Britschgi, Markus, Villeda, Saul, Zhang, Hui, Ding, Zhaoqing, Zhu, Liyin, Alabsi, Haitham, Getachew, Ruth, Narasimhan, Ramya, Wabl, Rafael, Fainberg, Nina, James, Michelle, Wong, Gordon, Relton, Jane, Gambhir, Sanjiv S, Pollard, Jeffrey, Wyss-Coray, Tony
    J Exp Med, 2013-01-07;210(1):157-72.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  7. Osteoblasts support B-lymphocyte commitment and differentiation from hematopoietic stem cells.
    Authors: Zhu J, Garrett R, Jung Y, Zhang Y, Kim N, Wang J, Joe GJ, Hexner E, Choi Y, Taichman RS, Emerson SG
    Blood, 2007-01-16;109(9):3706-12.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization

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