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Mouse MDL-1/CLEC5A PE-conjugated Antibody

Catalog #: FAB1639P
3 Citations Datasheet / COA / SDS
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FAB1639P

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Detection of MDL‑1/CLEC5A in RAW 264.7 Mouse Cell Line by Flow Cytometry.
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Citations (3)
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Mouse MDL-1/CLEC5A PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse MDL-1/CLEC5A. In direct ELISAs, approximately 50% cross-reactivity with recombinant human MDL‑1 is observed.
Source
Monoclonal Rat IgG2A Clone # 226402
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with mouse MDL-1/CLEC5A
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of MDL-1/CLEC5A antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of MDL‑1/CLEC5A in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse MDL-1/CLEC5A PE-conjugated Monoclonal Antibody (Catalog # FAB1639P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: MDL-1/CLEC5A

MDL-1 is an approximately 40 kDa transmembrane glycoprotein belonging to the C-type lectin superfamily (CLEC5A). MDL-1 is expressed on immature myeloid cells, monocytes, macrophages, dendritic cells, neutrophils, NK cells, and osteocytes. It contains a charged lysine in the transmembrane region that enables it to associate with DAP12 and deliver an activating signal. MDL-1 mediates inflammatory responses during autoimmune arthritis and upon binding to Dengue and Japanese encephalitis viruses. The extracellular domain (ECD) of MDL-1 contains a juxtamembrane stalk region and one C-type lectin domain. Within the ECD, mouse and human MDL-1 share 67% amino acid sequence identity. In mouse, alternative splicing generates a short isoform with a 25 aa deletion in the stalk region.

Long Name
Myeloid DAP12-associating Lectin
Entrez Gene IDs
23601 (Human); 23845 (Mouse)
Alternate Names
CLEC5A; CLECSF5; CLECSF5carbohydrate-recognition domain) lectin, superfamilymember 5; C-type lectin domain family 5, member A; C-type lectin superfamily member 5; MDL1; MDL-1; MDL-1MGC138304; Myeloid DAP12-associating lectin 1; myeloid DAP12-associating lectin-1

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Citations for Mouse MDL-1/CLEC5A PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. CLEC5a-directed bispecific antibody for effective cellular phagocytosis
    Authors: Vivekananda Kedage, Diego Ellerman, Mingjian Fei, Wei-Ching Liang, Gu Zhang, Eric Cheng et al.
    mAbs
  2. Single-Cell Transcriptomics of Human and Mouse Lung Cancers Reveals Conserved Myeloid Populations across Individuals and Species
    Authors: Rapolas Zilionis, Camilla Engblom, Christina Pfirschke, Virginia Savova, David Zemmour, Hatice D. Saatcioglu et al.
    Immunity
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Osteoblasts remotely supply lung tumors with cancer-promoting SiglecF high neutrophils
    Authors: Camilla Engblom, Christina Pfirschke, Rapolas Zilionis, Janaina da Silva Da Silva Martins, Stijn A. Bos, Gabriel Courties et al.
    Science
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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