Mouse Neuroplastin Antibody Summary
Gln29-Pro338, predicted
Accession # P97300
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse Neuroplastin by Western Blot. Western blot shows lysates of mouse brain (whole) tissue and mouse brain (cerebellum) tissue. PVDF membrane was probed with 0.05 µg/mL of Sheep Anti-Mouse Neuroplastin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7818) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neuroplastin at approximately 55-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neuroplastin
Neuroplastin (NPTN or neural plasticity-dependent synaptic rearrangement protein; also Np65 and SDR-1) is a 63-67 kDa member of the CD147 family, Ig‑superfamily of molecules. Its expression is primarily restricted to the PSD of select neurons, and it likely serves as a cell adhesion molecule between cells. NPTN is known to bind homotypically in trans, and this complex appears to interact with FGFR1 in cis. Engagement of the FGFR induces neurite outgrowth. Notably, rodent and human Np65 show distinctly different staining patterns in the hippocampus and cerebellum of the brain. Mature mouse NPTN is a 369 amino acid (aa) type I transmembrane glycoprotein. It possesses a 310 aa extracellular region (aa 29-338) that contains three Ig-like domains (aa 29-134; 148-234; 237-327), and a 38 aa cytoplasmic tail. There are three splice forms that have been described. One is a widely-expressed 53-57 kDa isoform (Np55) that contains a Glu substitution for aa 31‑147, a second is a splice form that shows the above substitution coupled to a deletion of aa 371-374, and a third is an isoform that contains a nine aa substitution for aa 1-235. Over aa 1-338, mouse NPTN shares 95% and 98% aa sequence identity with human and rat NPTN/Np65, respectively.
Product Datasheets
Citations for Mouse Neuroplastin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Erythrocyte invasion-neutralising antibodies prevent Plasmodium falciparum RH5 from binding to basigin-containing membrane protein complexes
Authors: Jamwal, A;Constantin, CF;Hirschi, S;Henrich, S;Bildl, W;Fakler, B;Draper, SJ;Schulte, U;Higgins, MK;
eLife
Species: Human
Sample Types: Cell Lysates
Applications: Affinity Purification -
MANF supports the inner hair cell synapse and the outer hair cell stereocilia bundle in the cochlea
Authors: Kuu Ikäheimo, Anni Herranen, Vilma Iivanainen, Tuuli Lankinen, Antti A Aarnisalo, Ville Sivonen et al.
Life Science Alliance
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Neuroplastin genetically interacts with Cadherin 23 and the encoded isoform Np55 is sufficient for cochlear hair cell function and hearing
Authors: Sherylanne Newton, Fanbo Kong, Adam J. Carlton, Carlos Aguilar, Andrew Parker, Gemma F. Codner et al.
PLOS Genetics
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Plasma Membrane Calcium ATPase-Neuroplastin Complexes Are Selectively Stabilized in GM1-Containing Lipid Rafts
Authors: Ilic K, Lin X, Malci A et al.
International Journal of Molecular Sciences
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Trans-differentiation of outer hair cells into inner hair cells in the absence of INSM1
Authors: T Wiwatpanit, SM Lorenzen, JA Cantú, CZ Foo, AK Hogan, F Márquez, JC Clancy, MJ Schipma, MA Cheatham, A Duggan, J García-Año
Nature, 2018-10-10;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Neuroplastin Isoform Np55 Is Expressed in the Stereocilia of Outer Hair Cells and Required for Normal Outer Hair Cell Function
J Neurosci, 2016-08-31;36(35):9201-16.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
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