Mouse NGFR/TNFRSF16 Antibody

Name: Mouse NGFR/TNFRSF16 Antibody
Brand: R&D Systems
SKU: AF1157
Rating: 4.7 (6 reviews)

Catalog #: AF1157
26 Citations 6 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1157
AF1157-SP

Detection of Mouse NGF R/TNFRSF16 by Western Blot.

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All NGFR/TNFRSF16 Antibodies

Product Details

Citations (26)

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Supplemental Products

Reviews (6)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse NGFR/TNFRSF16 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse NGF R/TNFRSF16 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human NGF R/TNFRSF16 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse NGF R/TNFRSF16
Gly20-Asn243
Accession # Q9Z0W1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse NGF R/TNFRSF16 by Western Blot. Western blot shows lysates of mouse uterus tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse NGF R/TNFRSF16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1157) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for NGF R/TNFRSF16 at approximately 60-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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NGF R/TNFRSF16 in Mouse Brain. NGF R/TNFRSF16 was detected in perfusion fixed frozen sections of mouse brain (cortex) using 7 µg/mL Goat Anti-Mouse NGF R/TNFRSF16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1157) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NGFR/TNFRSF16

The low affinity nerve growth factor receptor (NGF R), also named p75 neurotrophin receptor, is a type I transmembrane protein that belongs to the tumor necrosis factor receptor family and has been designated TNFRSF16. NGF R cDNA encodes a 427 amino acid (aa) residue precursor protein with a 28 aa residue signal peptide, a 222 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 155 aa residue intracellular domain. The extracellular region contains four cysteine-rich domains and binds NGF, BDNF, NT-3, and NT-4 approximately equally with low affinity. The cytoplasmic region of the receptor contains a subtype 2 death domain.

NGF R expression has been shown to occur widely during development and in the adult. Expression has been detected in both neuronal and non-neuronal cells. NGF R was originally reported to function as a positive regulator of TrkA activity. NGF R has also been shown to signal by itself. Depending on its cellular environment, NGF R has now been shown to regulate cell migration, gene expression and to mediate apoptosis. Recombinant NGF R Fc chimera binds NGF with high affinity and is a potent NGF antagonist. Naturally occurring truncated NGF R containing the extracellular domain and lacking the transmembrane or intracellular domain has been detected in vivo in urine, plasma, and in the amniotic fluid of humans and rats (1-3).

References

Barker, P.A. and R.A. Murphy (1992) Molecular and Cellular Biochemistry 110:1.
Bamji, A.X. et al. (1998) J. Cell Biol. 140:911.
Feinstein, E. et al. (1995) Trends Biochem. Sci. 20:342.

Long Name

Nerve Growth Factor Receptor

Entrez Gene IDs

4804 (Human); 18053 (Mouse); 117089 (Rat)

Alternate Names

CD271 antigen; CD271; Gp80-LNGFR; member 16; nerve growth factor receptor; NGF R; NGF receptor; NGFR; p75 ICD; p75 NTR; TNFRSF16; TNFRSF16nerve growth factor receptor (TNFR superfamily, member 16); tumor necrosis factor receptor superfamily member 16

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse NGFR/TNFRSF16 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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Regulation of BACE1 expression after injury is linked to the p75 neurotrophin receptor
Authors: Khalil Saadipour, Alexia Tiberi, Sylvia Lombardo, Elena Grajales, Laura Montroull, Noralyn B. Mañucat-Tan et al.
Molecular and Cellular Neuroscience
Blockade of TrkB but not p75 NTR activates a subpopulation of quiescent neural precursor cells and enhances neurogenesis in the adult mouse hippocampus
Authors: Natalie Groves, Imogen O’Keeffe, Wendy Lee, Alexandra Toft, Daniel Blackmore, Saurabh Bandhavkar et al.
Developmental Neurobiology
Cortical Brain Injury Causes Retrograde Degeneration of Afferent Basal Forebrain Cholinergic Neurons via the p75NTR
Authors: Srestha Dasgupta, Laura E. Montroull, Mansi A. Pandya, Juan P. Zanin, Wei Wang, Zhuhao Wu et al.
eNeuro
TrkB-containing exosomes promote the transfer of glioblastoma aggressiveness to YKL-40-inactivated glioblastoma cells
Authors: Sandra Pinet, Barbara Bessette, Nicolas Vedrenne, Aurélie Lacroix, Laurence Richard, Marie-Odile Jauberteau et al.
Oncotarget
Neurotrophic factor control of adult SVZ neurogenesis
Authors: Kevin G. Bath, Francis S. Lee
Developmental Neurobiology
Neuronal apoptosis induced by morphine withdrawal is mediated by the p75 neurotrophin receptor
Authors: Gino P. Asuni, Andrew Speidell, Italo Mocchetti
Journal of Neurochemistry
Proneurotrophins Induce Apoptotic Neuronal Death After Controlled Cortical Impact Injury in Adult Mice
Authors: Laura E. Montroull, Deborah E. Rothbard, Hur D. Kanal, Veera D'Mello, Vincent Dodson, Carol M. Troy et al.
ASN Neuro
p75 Neurotrophin Receptor Activation Regulates the Timing of the Maturation of Cortical Parvalbumin Interneuron Connectivity and Promotes Juvenile-like Plasticity in Adult Visual Cortex
Authors: Elie Baho, Bidisha Chattopadhyaya, Marisol Lavertu-Jolin, Raffaele Mazziotti, Patricia N. Awad, Pegah Chehrazi et al.
The Journal of Neuroscience
The p75 neurotrophin receptor is required for the survival of neuronal progenitors and normal formation of the basal forebrain, striatum, thalamus and neocortex
Authors: Sonja Meier, Fabienne Alfonsi, Nyoman D. Kurniawan, Michael R. Milne, Maria A. Kasherman, Alessio Delogu et al.
Development
Tiered sympathetic control of cardiac function revealed by viral tracing and single cell transcriptome profiling
Authors: Sharma S, Littman R, Tompkins JD et al.
eLife

Neuroimaging, Urinary, and Plasma Biomarkers of Treatment Response in Huntington’s Disease: Preclinical Evidence with the p75NTR Ligand LM11A-31
Authors: Danielle A. Simmons, Brian D. Mills, Robert R. Butler III, Jason Kuan, Tyne L. M. McHugh, Carolyn Akers et al.
Neurotherapeutics

Tiered sympathetic control of cardiac function revealed by viral tracing and single cell transcriptome profiling
Authors: Sharma S, Littman R, Tompkins JD et al.
eLife

Tiered Sympathetic Control of Cardiac Function Revealed by Viral Tracing and Single Cell Transcriptome Profiling
Authors: S Sharma, R Littman, J Tompkins, D Arneson, J Contreras, AH Dajani, K Ang, A Tsanhani, X Sun, PY Jay, H Herzog, X Yang, OA Ajijola
bioRxiv : the preprint server for biology, 2023-01-19;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Closing the Gap between the Auditory Nerve and Cochlear Implant Electrodes: Which Neurotrophin Cocktail Performs Best for Axonal Outgrowth and Is Electrical Stimulation Beneficial?
Authors: D Schmidbaue, S Fink, F Rousset, H Löwenheim, P Senn, R Glueckert
International Journal of Molecular Sciences, 2023-01-19;24(3):.
Species: Mouse
Sample Types: Organoid
Applications: IHC

Etiological Roles of p75NTR in a Mouse Model of Wet Age-Related Macular Degeneration
Authors: PV Subirada, A Tovo, MV Vaglienti, JD Luna Pinto, HU Saragovi, MC Sánchez, A Anastasía, PF Barcelona
Cells, 2023-01-12;12(2):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Sterol O-Acyltransferase Inhibition Ameliorates High-Fat Diet-Induced Renal Fibrosis and Tertiary Lymphoid Tissue Maturation after Ischemic Reperfusion Injury
Authors: Y Ariyasu, Y Sato, Y Isobe, K Taniguchi, M Yanagita, M Arita
International Journal of Molecular Sciences, 2022-12-07;23(24):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

A Novel Neuroprotective Mechanism for Lithium That Prevents Association of the p75NTR-Sortilin Receptor Complex and Attenuates proNGF-Induced Neuronal Death In Vitro and In Vivo
Authors: SG Greenwood, L Montroull, M Volosin, HE Scharfman, KK Teng, M Light, R Torkin, F Maxfield, BL Hempstead, WJ Friedman
eNeuro, 2018-01-17;5(1):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Urinary Extracellular Domain of Neurotrophin Receptor p75 as a Biomarker for Amyotrophic Lateral Sclerosis in a Chinese cohort
Authors: R Jia, S Shepheard, J Jin, F Hu, X Zhao, L Xue, L Xiang, H Qi, Q Qu, F Guo, ML Rogers, J Dang
Sci Rep, 2017-07-11;7(1):5127.
Species: Human
Sample Types: Urine
Applications: ELISA Development (Detection)

Superior Cervical Ganglia Neurons Induce Foxp3+ Regulatory T Cells via Calcitonin Gene-Related Peptide
Authors: K Szklany, E Ruiter, F Mian, W Kunze, J Bienenstoc, P Forsythe, K Karimi
PLoS ONE, 2016-03-29;11(3):e0152443.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

Semaphorin 3E secreted by damaged hepatocytes regulates the sinusoidal regeneration and liver fibrosis during liver regeneration.
Authors: Yagai T, Miyajima A, Tanaka M
Am J Pathol, 2014-06-12;184(8):2250-9.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

An intracellular domain fragment of the p75 neurotrophin receptor (p75(NTR)) enhances tropomyosin receptor kinase A (TrkA) receptor function.
Authors: Matusica, Dusan, Skeldal, Sune, Sykes, Alex M, Palstra, Nickless, Sharma, Aanchal, Coulson, Elizabet
J Biol Chem, 2013-03-07;288(16):11144-54.
Species: Human, Rat
Sample Types: Cell Lysates
Applications: Western Blot

Mouse natural killer (NK) cells express the nerve growth factor receptor TrkA, which is dynamically regulated.
Authors: Ralainirina N, Brons NH, Ammerlaan W, Hoffmann C, Hentges F, Zimmer J
PLoS ONE, 2010-12-01;5(12):e15053.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC

Long-term delivery of nerve growth factor by encapsulated cell biodelivery in the Gottingen minipig basal forebrain.
Authors: Fjord-Larsen L, Kusk P, Tornoe J, Juliusson B, Torp M, Bjarkam CR, Nielsen MS, Handberg A, Sorensen JC, Wahlberg LU
Mol. Ther., 2010-07-27;18(12):2164-72.
Species: Porcine
Sample Types: Whole Tissue
Applications: IHC-Fr

Human immunomodulatory ligand B7-1 mediates synaptic remodeling via the p75 neurotrophin receptor
Authors: Nicholas C. Morano, Roshelle S. Smith, Victor Danelon, Ryan Schreiner, Uttsav Patel, Natalia G. Herrera et al.
Journal of Clinical Investigation

Heterogeneous fibroblasts underlie age-dependent tertiary lymphoid tissues in the kidney
Authors: Yuki Sato, Akiko Mii, Yoko Hamazaki, Harumi Fujita, Hirosuke Nakata, Kyoko Masuda et al.
JCI Insight

BMSCs Promote Differentiation of Enteric Neural Precursor Cells to Maintain Neuronal Homeostasis in Mice With Enteric Nerve Injury
Authors: Mengke Fan, Huiying Shi, Hailing Yao, Weijun Wang, Yurui Zhang, Chen Jiang et al.
Cellular and Molecular Gastroenterology and Hepatology