Mouse Notch-3 Antibody Summary
Notch‑3 is observed and less than 5% cross-reactivity with recombinant rat (rr) Notch-1 and rrNotch-2 is observed.
Ala40-Glu468
Accession # Q61982
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Notch‑3 in CD4- CD8- mouse thymocytes by Flow Cytometry CD4- CD8- mouse thymocytes were stained with Rat Anti-Mouse CD25/IL-2R alpha PE‑conjugated Monoclonal Antibody (Catalog # FAB2438P) and either (A) Goat Anti-Mouse Notch‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1308) or (B) isotype control antibody (Catalog # AB-108-C) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.
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Detection of Notch‑3 in Mouse Embryo (E13). Notch‑3 was detected in immersion fixed frozen sections of mouse embryo (E13) using Goat Anti-Mouse Notch‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1308) at 1 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
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Detection of Notch‑3 in Mouse Embryo (E13). Notch‑3 was detected in immersion fixed frozen sections of mouse embryo (E13) using Goat Anti-Mouse Notch‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1308) at 3 µg/ml overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the cell surface. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Notch-3
Mouse Notch-3 is part of the Notch family of type I transmembrane glycoproteins involved in a number of early-event developmental processes (1). The extracellular domain of Notch receptors interact with the extracellular domain of transmembrane ligands Jagged, Delta, and Serrate expressed on the surface of a neighboring cell. In both vertebrates and invertebrates, Notch signaling is important for specifying cell fates and for defining boundaries between different cell types. The Notch molecule is synthesized as a 2318 amino acid (aa) precursor that contains an 39 aa signal sequence, a 1603 aa extracellular region, a 20 aa transmembrane (TM) segment and a 655 aa cytoplasmic domain. The large Notch extracellular domain has 34 EGF-like repeats followed by three notch/Lin-12 repeats (LNR) (2). The 11th and 12th EGF-like repeats of Notch have been shown to be both necessary and sufficient for binding the ligands Serrate and Delta, in Drosophila (3). Notch-3 has the same biochemical mechanism of signal tranduction as Notch-1, where a series of cleavage events result in the release of the Notch intracellular domain (NICD). NICD translocates into the nucleus and initiates transcription of Notch-responsive genes (4). Thus Notch acts as both a ligand-binding receptor and a nuclear factor that regulates transcription.
Notch-3 is predominantly expressed in the developing central nervous system of mice (2). Mutations in Notch-3 in humans cause an autosomal dominant condition called CADASIL (cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy). This disorder is characterized by recurrent ischemic strokes at an early age without any underlying vascular risk and progressive dementia. Nearly all mutations leading to this disorder are clustered in the first 5 EGF repeats of the Notch-3 gene (5). Mouse Notch-3 shows 90% aa identity to human Notch-3 and 96% to rat Notch-3 over the entire protein.
- Weinmaster, G. (2000) Curr. Opin. Genet. Dev. 10:363.
- Lardelli, M. et al. (1994) Mech Dev. 46:123.
- Rebay, I. et al. (1991) Cell 67:687.
- Mizutani, T. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9026.
- Joutel, A. and E. Tounier-Lasserve (1998) Stem Cell & Dev. Biol. 9:619.
Product Datasheets
Citations for Mouse Notch-3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 9
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Intrathymic Notch3 and CXCR4 combinatorial interplay facilitates T-cell leukemia propagation
Authors: F Ferrandino, G Bernardini, G Tsaouli, P Grazioli, AF Campese, C Noce, A Ciuffetta, A Vacca, ZM Besharat, D Bellavia, I Screpanti, MP Felli
Oncogene, 2018-07-23;0(0):.
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Single-Cell Spatial MIST for Versatile, Scalable Detection of Protein Markers
Authors: Meah, A;Vedarethinam, V;Bronstein, R;Gujarati, N;Jain, T;Mallipattu, SK;Li, Y;Wang, J;
Biosensors
Species: Mouse
Sample Types: Complex Sample Type
Applications: IHC -
Notch signalling drives synovial fibroblast identity and arthritis pathology
Authors: K Wei, I Korsunsky, JL Marshall, A Gao, GFM Watts, T Major, AP Croft, J Watts, PE Blazar, JK Lange, TS Thornhill, A Filer, K Raza, LT Donlin, CW Siebel, CD Buckley, S Raychaudhu, MB Brenner
Nature, 2020-04-22;582(7811):259-264.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Loss of the transcription factor RBPJ induces disease-promoting properties in brain pericytes
Authors: R Diéguez-Hu, K Kato, BD Giaimo, M Nieminen-K, H Arf, F Ferrante, M Bartkuhn, T Zimmermann, MG Bixel, HM Eilken, S Adams, T Borggrefe, P Vajkoczy, RH Adams
Nat Commun, 2019-06-27;10(1):2817.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Pulmonary pericytes regulate lung morphogenesis
Authors: K Kato, R Diéguez-Hu, DY Park, SP Hong, S Kato-Azuma, S Adams, M Stehling, B Trappmann, JL Wrana, GY Koh, RH Adams
Nat Commun, 2018-06-22;9(1):2448.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Involvement of Notch2 in all?trans retinoic acid?induced inhibition of mouse embryonic palate mesenchymal cell proliferation
Authors: Y Zhang, S Dong, J Wang, M Wang, M Chen, H Huang
Mol Med Rep, 2017-07-06;16(3):2538-2546.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
OX40L/Jagged1 Cosignaling by GM-CSF–Induced Bone Marrow-Derived Dendritic Cells Is Required for the Expansion of Functional Regulatory T Cells
Authors: Anupama Gopisetty, Palash Bhattacharya, Christine Haddad, Joseph C. Bruno, Chenthamarakshan Vasu, Lucio Miele et al.
The Journal of Immunology
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Canonical notch signaling functions as a commitment switch in the epidermal lineage.
Authors: Blanpain C, Lowry WE, Pasolli HA, Fuchs E
Genes Dev., 2006-11-01;20(21):3022-35.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Prolyl-isomerase Pin1 controls Notch3 protein expression and regulates T-ALL progression
Oncogene, 2016-02-15;35(36):4741-51.
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