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Mouse Osteopontin/OPN PE-conjugated Antibody

Catalog #: IC808P
13 Citations 1 Review Datasheet / COA / SDS
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IC808P
Detection of Osteopontin/OPN in RAW 264.7 Mouse Cell Line by Flow Cytometry.
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Citations (13)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Osteopontin/OPN PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Osteopontin (OPN) in ELISAs and Western blots. In sandwich ELISAs, less than 3% cross-reactivity with recombinant human OPN is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Osteopontin/OPN
Leu17-Asn294 (Glu99Gly)
Accession # Q547B5
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of Osteopontin/OPN antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Osteopontin/OPN in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line either (A) untreated or (B) treated with LPS was stained with Goat Anti-Mouse Osteopontin/OPN PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC808P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Osteopontin/OPN

Osteopontin (OPN, previously also referred to as transformation-associated secreted phosphoprotein, bone sialoprotein I, 2ar, 2B7, early T lymphocyte activation 1 protein, minopotin, calcium oxalate crystal growth inhibitor protein), is a secreted, highly acidic, calcium-binding, RGD-containing, phosphorylated glycoprotein originally isolated from bone matrix (1). Subsequently, OPN has been found in kidney, placenta, blood vessels and various tumor tissues. Many cell types (including macrophages, osteoclasts, activated T cells, fibroblasts, epithelial cells, vascular smooth muscle cells, and natural killer cells) can express OPN in response to activation by cytokines, growth factors or inflammatory mediators. Elevated expression of OPN has also been associated with numerous pathobiological conditions such as atherosclerotic plaques, renal tubulointerstitial fibrosis, granuloma formations in tuberculosis and silicosis, neointimal formation associated with balloon catheterization, metastasizing tumors, and cerebral ischemia. Mouse OPN cDNA encodes a 294 amino acid (aa) residue precursor protein with a 16 aa residue predicted signal peptide that is cleaved to yield a 278 aa residue mature protein with an integrin binding sequence (RGD), and N- and O-glycosylation sites. OPN has been shown to bind to different cell types through RGD-mediated interaction with the integrins alpha v beta 1, alpha v beta 3, alpha v beta 5, and non-RGD-mediated interaction with CD44 and the integrins alpha 8 beta 1 or alpha 9 beta 1. Functionally, OPN is chemotactic for macrophages, smooth muscle cells, endothelial cells and glial cells. OPN has also been shown to inhibit nitric oxide production and cytotoxicity by activated macrophages. Human, mouse, rat, pig and bovine OPN share from approximately 40-80% amino acid sequence identity. Osteopontin is a substrate for proteolytic cleavage by thrombin, enterokinase, MMP-3 and MMP-7. The functions of OPN in a variety of cell types were shown to be modified as a result of proteolytic cleavage (2, 3).

References
  1. Ann. N.Y. Acad. Sci., vol. 760, 1995, Apr. 21.
  2. Senger, D.R. et al. (1996) Biochim. Biophys. Acta. 1314:13.
  3. Agnihotri, R. et al. (2001) J. Biol. Chem. 276:28261.
Long Name
Secreted Phosphoprotein 1 [BNSP]
Entrez Gene IDs
6696 (Human); 20750 (Mouse); 25353 (Rat); 281499 (Bovine)
Alternate Names
BNSP; Bone sialoprotein 1; Eta-1; MGC110940; Nephropontin; OPN; Osteopontin; secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1); secreted phosphoprotein-1 (osteopontin, bone sialoprotein); Spp1; SPP-1; SPP1/CALPHA1 fusion; Urinary stone protein; uropontin

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Citations for Mouse Osteopontin/OPN PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Treg cell-derived osteopontin promotes microglia-mediated white matter repair after ischemic stroke
    Authors: Ligen Shi, Zeyu Sun, Wei Su, Fei Xu, Di Xie, Qingxiu Zhang et al.
    Immunity
  2. TET2-mediated mRNA demethylation regulates leukemia stem cell homing and self-renewal
    Authors: Li, Y;Xue, M;Deng, X;Dong, L;Nguyen, LXT;Ren, L;Han, L;Li, C;Xue, J;Zhao, Z;Li, W;Qing, Y;Shen, C;Tan, B;Chen, Z;Leung, K;Wang, K;Swaminathan, S;Li, L;Wunderlich, M;Mulloy, JC;Li, X;Chen, H;Zhang, B;Horne, D;Rosen, ST;Marcucci, G;Xu, M;Li, Z;Wei, M;Tian, J;Shen, B;Su, R;Chen, J;
    Cell stem cell
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  3. Dietary phosphorus consumption alters T cell populations, cytokine production, and bone volume in mice
    Authors: JL Roberts, M Yu, M Viggeswara, JL Arnst, R Pacifici, GR Beck
    JCI Insight, 2023-05-22;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Definition of the contribution of an Osteopontin-producing CD11c+ microglial subset to Alzheimer's disease
    Authors: Y Qiu, X Shen, O Ravid, D Atrakchi, D Rand, AE Wight, HJ Kim, S Liraz-Zalt, I Cooper, M Schnaider, H Cantor
    Proceedings of the National Academy of Sciences of the United States of America, 2023-02-02;120(6):e2218915120.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. The antioxidant enzyme Peroxiredoxin-1 controls stroke-associated microglia against acute ischemic stroke
    Authors: S Kim, W Lee, H Jo, SK Sonn, SJ Jeong, S Seo, J Suh, J Jin, HY Kweon, TK Kim, SH Moon, S Jeon, JW Kim, YR Kim, EW Lee, HK Shin, SH Park, GT Oh
    Redox Biology, 2022-05-25;54(0):102347.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Definition of a mouse microglial subset that regulates neuronal development and proinflammatory responses in the brain
    Authors: X Shen, Y Qiu, AE Wight, HJ Kim, H Cantor
    Proceedings of the National Academy of Sciences of the United States of America, 2022-02-22;119(8):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. NK cells induce hepatic ER stress to promote insulin resistance in obesity through osteopontin production
    Authors: J Wu, D Wu, L Zhang, C Lin, J Liao, R Xie, Z Li, S Wu, A Liu, W Hu, Y Xi, S Bu, F Wang
    J. Leukoc. Biol., 2019-12-12;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. An osteopontin/CD44 immune checkpoint controls CD8+ T cell activation and tumor immune evasion
    Authors: JD Klement, AV Paschall, PS Redd, ML Ibrahim, C Lu, D Yang, E Celis, SI Abrams, K Ozato, K Liu
    J. Clin. Invest., 2018-11-05;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. The tumor suppressor menin prevents effector CD8 T-cell dysfunction by targeting mTORC1-dependent metabolic activation
    Authors: J Suzuki, T Yamada, K Inoue, S Nabe, M Kuwahara, N Takemori, A Takemori, S Matsuda, M Kanoh, Y Imai, M Yasukawa, M Yamashita
    Nat Commun, 2018-08-17;9(1):3296.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Osteopontin attenuates aging-associated phenotypes of hematopoietic stem cells
    Authors: N Guidi, M Sacma, L Ständker, K Soller, G Marka, K Eiwen, JM Weiss, F Kirchhoff, T Weil, JA Cancelas, MC Florian, H Geiger
    EMBO J, 2017-03-02;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. The Menin-Bach2 axis is critical for regulating CD4 T-cell senescence and cytokine homeostasis.
    Authors: Kuwahara M, Suzuki J, Tofukuji S, Yamada T, Kanoh M, Matsumoto A, Maruyama S, Kometani K, Kurosaki T, Ohara O, Nakayama T, Yamashita M
    Nat Commun, 2014-04-02;5(0):3555.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Transgenic Overexpression of Tissue‐Nonspecific Alkaline Phosphatase (TNAP) in Vascular Endothelium Results in Generalized Arterial Calcification
    Authors: Alexei Y. Savinov, Maryam Salehi, Manisha C. Yadav, Ilian Radichev, José Luis Millán, Olga V. Savinova
    Journal of the American Heart Association
  13. CD153/CD30 signaling promotes age-dependent tertiary lymphoid tissue expansion and kidney injury
    Authors: Yuki Sato, Akiko Oguchi, Yuji Fukushima, Kyoko Masuda, Naoya Toriu, Keisuke Taniguchi et al.
    Journal of Clinical Investigation

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Mouse Osteopontin/OPN PE-conjugated Antibody
By Sul A Lee on 01/02/2019
Application: Flow Sample Tested: T cells Species: Mouse

I used this antibody to measure the level of ostepontin in renal T cells using flow cytometry.
However, it didn't work and this antibody didn't even bind with the compensation beads.

Flow Cytometry Mouse Osteopontin/OPN PE-conjugated Antibody IC808P