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Mouse Peptidase Inhibitor 16/PI16 Antibody

Catalog #: AF4929
5 Citations Datasheet / COA / SDS
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AF4929
AF4929-SP
Product Details
Citations (5)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Peptidase Inhibitor 16/PI16 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Peptidase Inhibitor 16/PI16 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Peptidase Inhibitor 16/PI16
Leu21-Glu464
Accession # Q9ET66
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Peptidase Inhibitor 16/PI16

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Peptidase Inhibitor 16/PI16

PI16 (Peptidase Inhibitor 16) is a variable molecular weight (MW) member of the CRISP family of proteins. It is expressed by cardiomyocytes and serves as an autocrine negative growth regulator. Mature mouse PI16 is a type I transmembrane protein 469 amino acids (aa) in length. The extracellular region includes all but the C‑terminal 21 amino acids. A soluble form of PI16 is known. Although its predicted MW is 50 kDa, it runs anomalously at 70 kDa (unmodified) in SDS-PAGE. With glycosylation, it appears to be 100‑110 kDa in size. Three potential variants exist. One shows a deletion of aa 51‑124, a second shows a 9 aa extension at the N‑terminus, and a third shows a deletion of aa 191‑452. Over aa 1‑464, mouse PI16 shares 86% and 61% aa identity with rat and human PI16, respectively.

Entrez Gene IDs
221476 (Human); 74116 (Mouse); 294312 (Rat)
Alternate Names
CRISP9; CRISP-9; Cysteine-rich secretory protein 9; dJ90K10.5; DKFZp586B1817; MGC45378; microseminoprotein, beta-binding protein; MSMBBP; Peptidase Inhibitor 16; PI16; PI-16; protease inhibitor 16; PSP94-binding protein; PSPBP

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Citations for Mouse Peptidase Inhibitor 16/PI16 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. The fibroblast-derived protein PI16 controls neuropathic pain
    Authors: Pooja Singhmar, Ronnie The Phong Trinh, Jiacheng Ma, XiaoJiao Huo, Bo Peng, Cobi J. Heijnen et al.
    Proceedings of the National Academy of Sciences
  2. Senescent fibroblasts in the tumor stroma rewire lung cancer metabolism and plasticity
    Authors: Lee, JY;Reyes, N;Woo, SH;Goel, S;Stratton, F;Kuang, C;Mansfield, AS;LaFave, LM;Peng, T;
    bioRxiv : the preprint server for biology
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  3. Fibroblast-derived PI16 sustains inflammatory pain via regulation of CD206+ myeloid cells
    Authors: Garrity, R;Arora, N;Areeful Haque, M;Weis, D;Trinh, RT;Neerukonda, SV;Kumari, S;Cortez, I;Ubogu, EE;Mahalingam, R;Tavares-Ferreira, D;Price, TJ;Kavelaars, A;Heijnen, CJ;Shepherd, AJ;
    Brain, behavior, and immunity
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  4. Elevated expression of activins promotes muscle wasting and cachexia.
    Authors: Chen J, Walton K, Winbanks C, Murphy K, Thomson R, Makanji Y, Qian H, Lynch G, Harrison C, Gregorevic P
    FASEB J, 2013-12-30;28(4):1711-23.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Global gene expression profiling in PAI-1 knockout murine heart and kidney: molecular basis of cardiac-selective fibrosis.
    Authors: Ghosh, Asish K, Murphy, Sheila B, Kishore, Raj, Vaughan, Douglas
    PLoS ONE, 2013-05-28;8(5):e63825.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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