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Mouse PGCP Antibody

Catalog #: AF4258 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4258
AF4258-SP
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse PGCP Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse PGCP in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse PGCP
Lys19-Ser470
Accession # Q9WVJ3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse PGCP
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse PGCP, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PGCP

PGCP (plasma glutamate carboxypeptidase) is a secreted member of the M28 family of metallopeptidases. Mouse PGCP is 470 amino acids (aa) in length. It apparently contains one annexin-like region (aa 19‑68) plus a peptidase M28 catalytic domain (aa 281-443). It appears to be synthesized by the liver. Based on human, mouse PGCP will show a 97 aa substitution for the C-terminal 26 amino acids. Over aa’s 19‑470, mouse PGCP shares 89% and 94% aa identity with human and rat PGCP, respectively.

Long Name
Plasma Glutamate Carboxypeptidase
Entrez Gene IDs
10404 (Human); 54381 (Mouse); 58952 (Rat)
Alternate Names
aminopeptidase; CPQ; EC 3.4.17; EC 3.4.17.-; Hls2; Lal-1; PGCP; plasma glutamate carboxypeptidase

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