Mouse Plasma Kallikrein/KLKB1 Antibody

Catalog #: MAB2498 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2498
MAB2498-SP

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Mouse Plasma Kallikrein/KLKB1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Plasma Kallikrein/KLKB1 in direct ELISAs and Western blots. In Western blots, no cross‑reactivity with recombinant human (rh) Kallikrein/KLKB1, rhKallikrein 1, 3, 4, 5, 7, 8, 10, 11, 12, 13, 14, 15, recombinant mouse HGFA, rhFactor VI, rhFactor X, rhFactor XI, rhThrombin, or rhuPA is observed.

Source

Monoclonal Rat IgG₁ Clone # 337801

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Kallikrein/KLKB1
Gly20-Ala638
Accession # NP_032481

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Plasma Kallikrein/KLKB1 (Catalog # 2498-SE) under non-reducing conditions only

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Plasma Kallikrein/KLKB1

Plasma kallikrein, a serine protease, is synthesized in the liver and circulates in the plasma by binding to high molecular weight (HMW) kininogen or as a free zymogen. Once activated by its physiological activator, factor XII, it displays endopeptidase activity towards peptide bonds after arginine (preferred) and lysine. It cleaves HMW kininogen, its major physiological substrate, to release the potent vasodilator peptide bradykinin. It is also able to cleave a number of inactive precursor proteins to generate active products, such as plasminogen and prourokinase. Thus, it plays an important role in blood pressure regulation, fibrinolysis, and neutrophil activation (1). Mouse plasma kallikrein precursor contains a signal peptide (aa 1‑19) and a pro form sequence (aa 20‑638). Upon activation, the pro form is converted to a heavy chain and a light chain, which is linked by disulfide bonds and the latter contains the catalytic domain (2).

References

Colman, R. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 1644.
Seidah, N. et al. (1990) DNA and Cell Biology 9:737.

Entrez Gene IDs

3818 (Human); 16621 (Mouse)

Alternate Names

EC 3.4.21; EC 3.4.21.34; Fletcher factor; kallikrein B, plasma (Fletcher factor) 1; kininogenin; KLK3plasma kallikrein; KLKB1; plasma kallikrein heavy chain; plasma kallikrein light chain; Plasma Kallikrein; Plasma Prekallikrein; PPK