Mouse Plasma Kallikrein/KLKB1 Antibody Summary
Gly20-Ala638
Accession # NP_032481
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Plasma Kallikrein/KLKB1
Plasma Kallikrein, a serine protease, is synthesized in the liver and circulates in the plasma by binding to high molecular weight (HMW) kininogen or as a free zymogen. Once activated by its physiological activator, factor XII, it displays endopeptidase activity towards peptide bonds after arginine (preferred) and lysine. It cleaves HMW kininogen, its major physiological substrate, to release the potent vasodilator peptide bradykinin. It is also able to cleave a number of inactive precursor proteins to generate active products, such as plasminogen and prourokinase. Thus, it plays an important role in blood pressure regulation, fibrinolysis, and neutrophil activation (1). Mouse Plasma Kallikrein precursor contains a signal peptide (residues 1 to 19) and a pro form sequence (residues 20 to 638). Upon activation, the pro form is converted to a heavy chain and a light chain, which is linked by disulfide bonds and the latter contains the catalytic domain (2). The mouse Plasma Kallikrein pro form was expressed in the NS0 cells with a foreign signal peptide. After being treated by thermolysin, the purified enzyme is active against a fluorogenic peptide substrate.
- Colman, R. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 1644.
- Seidah, N. et al. (1990) DNA and Cell Biology 9:737.
Product Datasheets
Citations for Mouse Plasma Kallikrein/KLKB1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Prekallikrein inhibits innate immune signaling in the lung and impairs host defense during pneumosepsis in mice
Authors: Chao Ding, Brendon P Scicluna, Ingrid Stroo, Jack Yang, Joris JTH Roelofs, Onno J de Boer et al.
The Journal of Pathology
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Limited role of kininogen in the host response during gram-negative pneumonia-derived sepsis
Authors: Chao Ding, Cornelis van ’t Veer, Joris J. T. H. Roelofs, Meenal Shukla, Keith R. McCrae, Alexey S. Revenko et al.
American Journal of Physiology-Lung Cellular and Molecular Physiology
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The Plasma Kallikrein-Kininogen Pathway Is Critical in the Pathogenesis of Colitis in Mice
Authors: B Wang, A Yang, Z Zhao, C He, Y Liu, RW Colman, J Dai, Y Wu
Front Immunol, 2018-02-06;9(0):21.
Species: Mouse
Sample Types: Plasma
Applications: Western Blot -
Active plasma kallikrein localizes to mast cells and regulates epithelial cell apoptosis, adipocyte differentiation, and stromal remodeling during mammary gland involution.
Authors: Lilla JN, Joshi RV, Craik CS, Werb Z
J. Biol. Chem., 2009-03-18;284(20):13792-803.
Species: Mouse
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC-P, Western Blot
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