Mouse Pro-MMP‑9 Antibody

Catalog #: MAB9092
5 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9092
MAB9092-SP

All MMP-9 Antibodies

Product Details

Citations (5)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Pro-MMP‑9 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Pro-MMP-9 in ELISAs. This antibody does not recognize the mature form of Pro-MMP-9.

Source

Monoclonal Rat IgG_2A Clone # 116134

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse MMP‑9
Ala20-Pro730
Accession # P41245.1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Mouse Pro-MMP-9 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Mouse Pro-MMP‑9 Biotinylated Antibody (Catalog # BAM909)

Standard: Recombinant Mouse MMP-9 Protein, CF (Catalog # 909-MM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-9

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 (gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-9 is produced by keratinocytes, monocytes, macrophages and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. Structurally, MMP-9 may be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin-binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline-rich linker region, and a carboxyl terminal hemopexin-like domain. Compared to the human MMP-9 (Catalog # 911-MP), the mouse enzyme contains extra sequences in the linker region and in the hemopexin-like domain, respectively.

Long Name

Matrix Metalloproteinase 9

Entrez Gene IDs

4318 (Human); 17395 (Mouse); 81687 (Rat); 102117693 (Cynomolgus Monkey)

Alternate Names

92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 3.4.24.35; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Mouse Pro-MMP‑9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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89Zr-pro-MMP-9 F(ab')2 detects colitis induced intestinal and kidney fibrosis
Authors: N Dmochowska, W Tieu, MD Keller, CA Hollis, MA Campaniell, C Mavrangelo, P Takhar, PA Hughes
Sci Rep, 2020-11-23;10(1):20372.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis.
Authors: Raghuwanshi S, Smith N, Rivers E, Thomas A, Sutton N, Hu Y, Mukhopadhyay S, Chen X, Leung T, Richardson R
J Immunol, 2013-04-15;190(10):5329-36.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Development (Capture)
Brucella abortus induces TNF-alpha-dependent astroglial MMP-9 secretion through mitogen-activated protein kinases.
Authors: Miraglia M, Scian R, Samartino C, Barrionuevo P, Rodriguez A, Ibanez A, Coria L, Velasquez L, Baldi P, Cassataro J, Delpino M, Giambartolomei G
J Neuroinflammation, 2013-04-12;10(0):47.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Capture)
Matrix metalloproteinase 9 facilitates West Nile virus entry into the brain.
Authors: Wang P, Dai J, Bai F, Kong KF, Wong SJ, Montgomery RR, Madri JA, Fikrig E
J. Virol., 2008-07-16;82(18):8978-85.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
Matrix metalloproteinase-8 facilitates neutrophil migration through the corneal stromal matrix by collagen degradation and production of the chemotactic peptide Pro-Gly-Pro.
Authors: Lin M, Jackson P, Tester AM, Diaconu E, Overall CM, Blalock JE, Pearlman E
Am. J. Pathol., 2008-06-13;173(1):144-53.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot