Home / Progranulin/PGRN / Mouse Progranulin/PGRN Antibody

Mouse Progranulin/PGRN Antibody

Catalog #: MAB2557
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2557
MAB2557-SP
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Progranulin/PGRN Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Progranulin/PGRN in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human Progranulin is observed.
Source
Monoclonal Rat IgG2A Clone # 333708
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Progranulin/PGRN
Thr18-Leu589
Accession # P28798
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample

Mouse Progranulin Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse Progranulin/PGRN Biotinylated Antibody (Catalog # BAM25572)

Standard: Recombinant Mouse Progranulin Protein, CF (Catalog # 2557-PG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Progranulin/PGRN

Progranulin, also known as acrogranin, PC cell-derived growth factor (PCDGF) and epithelin/granulin precursor, is a ubiquitously expressed, 88 kDa, secreted glycoprotein (1‑3). Structurally, it belongs to none of the well-established growth factor families (4). Mouse Progranulin is 589 amino acids (aa) in length and contains a 17 aa signal sequence and a 572 aa mature region that has four potential sites for N-linked glycosylation. It has a highly repetitive organization, containing seven tandem copies of a 55‑57 aa consensus motif that contains 12 conserved cysteine residues: VXCX5-6CX5CCX8CCX6CCXDX2HCCPX4CX5-6CX2 (1). Progranulin is secreted in an intact form (2, 4) or undergoes proteolysis leading to the release of multiple peptides made from the seven tandem repeats, the granulins (5‑7). Mouse Progranulin shares 87% and 75% aa sequence identity with rat and human Progranulin, respectively. Progranulin is involved in the regulation of cellular proliferation, as well as differentiation, development, and pathological processes (4). It has been isolated as a differentially expressed gene during mesothelial differentiation (8), macrophage development (9), development synovium of rheumatoid arthritis and osteoarthritis (10), sexual differentiation of the brain (11), and has also been shown to be a mediator of cartilage proliferation plus of wound response and tissue repair (4, 12‑13). High levels of Progranulin expression have been found to be associated with several human cancers, and are believed to contribute to tumorigenesis in breast cancer, clear cell renal carcinoma, invasive ovarian carcinoma, glioblastoma, adipocyte teratoma, and multiple myeloma (4, 5, 12, 14‑20).

References
  1. Plowman, G.D. et al. (1992) J. Biol. Chem. 267:13073. 
  2. Zhou, J. et al. (1993) J. Biol. Chem. 268:10863. 
  3. Liu, Y. et al. (2007) BMC Cancer 7:22. 
  4. Xu, K. et al. (2007) J. Biol. Chem. 282:11347. 
  5. Davidson, B. et al. (2004) Cancer 100:2139. 
  6. Zanocco-Marani, T. et al. (1999) Cancer Res. 59:5331. 
  7. Lu, R. and G. Serrero (2000) Proc. Natl. Acad. Sci. U.S.A. 97:3993. 
  8. Sun, X. et al. (2004) Am. J. Respir. Cell Mol. Biol. 30:510. 
  9. Barreda, D.R. et al. (2004) Dev. Comp. Immunol. 28:727 
  10. Justen, H.P. et al. (2000) Mol. Cell Biol. Res. Commun. 3:165.
  11. Suzuki, M. and M. Nishiahara (2002) Mol. Genet. Metab. 75:31.
  12. He, Z. et al. (2003) Nat. Med. 9:225.
  13. Zhu, J. et al. (2002) Cell 111:867.
  14. He, Z. and A. Bateman (2003) J. Mol. Med. 81:600.
  15. Bateman, A. et al. (1990) Biochem. Biophys. Res. Commun. 173:1161.
  16. Gonzalez, E.M. et al. (2003) J. Biol. Chem. 278:38113.
  17. Jones, M.B. et al. (2003) Gynecol. Oncol. 88:S136.
  18. Wang, W. et al. (2003) Clin. Cancer Res. 9:2221.
  19. Zhang, H. and G. Serrero (1998) Proc. Natl. Acad. Sci. U.S.A. 95:14202.
Entrez Gene IDs
2896 (Human); 14824 (Mouse)
Alternate Names
Acrogranin; CLN11; GEP; GP88; granulin precursor; GRN; PCDGF; PEPI; PGRN; Proepithelin; Progranulin

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Citation for Mouse Progranulin/PGRN Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. AZP2006, a new promising treatment for Alzheimer's and related diseases
    Authors: N Callizot, C Estrella, S Burlet, A Henriques, C Brantis, M Barrier, ML Campanari, P Verwaerde
    Scientific Reports, 2021-08-19;11(1):16806.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization

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