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Mouse Progranulin/PGRN Antibody

Catalog #: MAB2557R Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB2557R-100
MAB2557R-SP
Mouse Progranulin/PGRN ELISA Standard Curve.
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Mouse Progranulin/PGRN Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Progranulin/PGRN in direct ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human Progranulin is observed.
Source
Recombinant Monoclonal Rat IgG1 Clone # 333708R
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Progranulin/PGRN
Thr18-Leu589
Accession # P28798
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA capture antibody when paired with Rat Anti-Mouse Progranulin/PGRN Monoclonal Antibody (Catalog # MAB25572).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse Progranulin DuoSet ELISA Kit (Catalog # DY2557) for convenient development of a sandwich ELISA.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA Mouse Progranulin/PGRN Antibody in ELISA Standard Curve. View Larger

Mouse Progranulin/PGRN ELISA Standard Curve. Recombinant Mouse Progranulin/PGRN protein was serially diluted 2-fold and captured by Rat Anti-Mouse Progranulin/PGRN Monoclonal Antibody (Catalog # MAB2557R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse Progranulin/PGRN Monoclonal Antibody (Catalog # MAB25572) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Progranulin/PGRN

Progranulin, also known as acrogranin, PC cell-derived growth factor (PCDGF) and epithelin/granulin precursor, is a ubiquitously expressed, 88 kDa, secreted glycoprotein (1‑3). Structurally, it belongs to none of the well-established growth factor families (4). Mouse Progranulin is 589 amino acids (aa) in length and contains a 17 aa signal sequence and a 572 aa mature region that has four potential sites for N-linked glycosylation. It has a highly repetitive organization, containing seven tandem copies of a 55‑57 aa consensus motif that contains 12 conserved cysteine residues: VXCX5-6CX5CCX8CCX6CCXDX2HCCPX4CX5-6CX2 (1). Progranulin is secreted in an intact form (2, 4) or undergoes proteolysis leading to the release of multiple peptides made from the seven tandem repeats, the granulins (5‑7). Mouse Progranulin shares 87% and 75% aa sequence identity with rat and human Progranulin, respectively. Progranulin is involved in the regulation of cellular proliferation, as well as differentiation, development, and pathological processes (4). It has been isolated as a differentially expressed gene during mesothelial differentiation (8), macrophage development (9), development synovium of rheumatoid arthritis and osteoarthritis (10), sexual differentiation of the brain (11), and has also been shown to be a mediator of cartilage proliferation plus of wound response and tissue repair (4, 12‑13). High levels of Progranulin expression have been found to be associated with several human cancers, and are believed to contribute to tumorigenesis in breast cancer, clear cell renal carcinoma, invasive ovarian carcinoma, glioblastoma, adipocyte teratoma, and multiple myeloma (4, 5, 12, 14‑20).

References
  1. Plowman, G.D. et al. (1992) J. Biol. Chem. 267:13073. 
  2. Zhou, J. et al. (1993) J. Biol. Chem. 268:10863. 
  3. Liu, Y. et al. (2007) BMC Cancer 7:22. 
  4. Xu, K. et al. (2007) J. Biol. Chem. 282:11347. 
  5. Davidson, B. et al. (2004) Cancer 100:2139. 
  6. Zanocco-Marani, T. et al. (1999) Cancer Res. 59:5331. 
  7. Lu, R. and G. Serrero (2000) Proc. Natl. Acad. Sci. U.S.A. 97:3993. 
  8. Sun, X. et al. (2004) Am. J. Respir. Cell Mol. Biol. 30:510. 
  9. Barreda, D.R. et al. (2004) Dev. Comp. Immunol. 28:727 
  10. Justen, H.P. et al. (2000) Mol. Cell Biol. Res. Commun. 3:165.
  11. Suzuki, M. and M. Nishiahara (2002) Mol. Genet. Metab. 75:31.
  12. He, Z. et al. (2003) Nat. Med. 9:225.
  13. Zhu, J. et al. (2002) Cell 111:867.
  14. He, Z. and A. Bateman (2003) J. Mol. Med. 81:600.
  15. Bateman, A. et al. (1990) Biochem. Biophys. Res. Commun. 173:1161.
  16. Gonzalez, E.M. et al. (2003) J. Biol. Chem. 278:38113.
  17. Jones, M.B. et al. (2003) Gynecol. Oncol. 88:S136.
  18. Wang, W. et al. (2003) Clin. Cancer Res. 9:2221.
  19. Zhang, H. and G. Serrero (1998) Proc. Natl. Acad. Sci. U.S.A. 95:14202.
Entrez Gene IDs
2896 (Human); 14824 (Mouse)
Alternate Names
Acrogranin; CLN11; GEP; GP88; granulin precursor; GRN; PCDGF; PEPI; PGRN; Proepithelin; Progranulin

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