Mouse Progranulin ELISA Kit - Quantikine

Name: Mouse Progranulin Quantikine ELISA Kit
Brand: R&D Systems
SKU: MPGRN0
Rating: 5 (1 reviews)

Catalog #: MPGRN0
4 Citations 1 Review Datasheet / COA / SDS

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MPGRN0

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Product Details

Citations (4)

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Reviews (1)

Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Mouse Progranulin Quantikine ELISA Kit Summary

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Tissue Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

0.071 ng/mL

Assay Range

0.6 - 20 ng/mL (Cell Culture Supernates, Tissue Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant mouse Progranulin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine^® Mouse Progranulin Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse Progranulin in cell culture supernates, tissue culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse Progranulin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural Progranulin showed linear curves that were parallel to the standard curves obtained using the Quantikine^® kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Progranulin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians

Cell Culture Supernates, Tissue Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	1.75	2.94	7.28	1.69	2.89	7.53
Standard Deviation	0.05	0.067	0.16	0.092	0.137	0.336
CV%	2.9	2.3	2.2	5.4	4.7	4.5

Recovery

The recovery of mouse Progranulin spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Culture Media (n=4)	98	94-104

Linearity

To assess the linearity of the assay, samples containing high concentrations of mouse Progranulin were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.

Scientific Data

N/A

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Mouse Progranulin ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Progranulin/PGRN

Progranulin, also known as Acrogranin, PCDGF, and Epithelin/Granulin Precursor, is a widely expressed glycoprotein that can undergo proteolysis to generate numerous Granulin peptides. Progranulin plays a role in the regulation of mesothelial differentiation, macrophage development, sexual differentiation of the brain, cartilage proliferation, wound repair, and the development of rheumatoid arthritis and osteoarthritis. It is often upregulated in cancer and contributes to tumor progression. Progranulin upregulation or mutation is associated with several neurological disorders including frontotemporal dementia, Creutzfeldt-Jakob disease, Alzheimer’s disease, and motor neuron disease.

Entrez Gene IDs:

2896 (Human); 14824 (Mouse)

Alternate Names:

Acrogranin; CLN11; GEP; GP88; granulin precursor; GRN; PCDGF; PEPI; PGRN; Proepithelin; Progranulin

Related Research Areas

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Mouse Progranulin Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Increased circulating progranulin is not sufficient to induce cardiac dysfunction or supraventricular arrhythmia
Authors: McElhanon, KE;Huff, TC;Hirenallur-Shanthappa, D;Miller, RA;Christoforou, N;
Scientific reports
Species: Mouse
Sample Types: Plasma
Progranulin-derived granulin E and lysosome membrane protein CD68 interact to reciprocally regulate their protein homeostasis
Authors: MN Santos, DH Paushter, T Zhang, X Wu, T Feng, J Lou, H Du, SM Becker, R Fragoza, H Yu, F Hu
The Journal of Biological Chemistry, 2022-08-04;0(0):102348.
Species: Mouse
Sample Types: Tissue Homogenates
Early Reciprocal Effects in a Murine Model of Traumatic Brain Injury and Femoral Fracture
Authors: K Ritter, K Jung, C Dolderer, D Appel, CC Oswald, U Ritz, MKE Schäfer
Mediators of Inflammation, 2021-01-18;2021(0):8835730.
Species: Mouse
Sample Types: Plasma
Anti-sortilin1 Antibody Up-Regulates Progranulin via Sortilin1 Down-Regulation
Authors: S Miyakawa, H Sakuma, D Warude, S Asanuma, N Arimura, T Yoshihara, D Tavares, A Hata, K Ida, Y Hori, Y Okuzono, S Yamamoto, K Iida, H Shimizu, S Kondo, S Sato
Frontiers in Neuroscience, 2020-12-15;14(0):586107.
Species: Mouse
Sample Types: Plasma