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Mouse/Rat Nestin Antibody

Catalog #: MAB2736
27 Citations 3 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2736
MAB2736-SP
Nestin in Rat Cortical Stem Cells.
3 Images
Product Details
Citations (27)
FAQs
Supplemental Products
Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat Nestin Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat Nestin in Western blots.
Source
Monoclonal Mouse IgG2A Clone # 307501
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant rat Nestin
Met544-Glu820 (Gly756Asp, Ile758Met, Arg572Lys, Ala574Pro, Ile802Met, Arg816Lys)
Accession # EDM00749
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Rat Nestin
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Nestin antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC). View Larger

Nestin in Rat Cortical Stem Cells. Nestin was detected in immersion fixed undifferentiated rat cortical stem cells using Mouse Anti-Rat Nestin Monoclonal Antibody (Catalog # MAB2736) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry View Larger

Nestin in C2C12 Mouse Cell Line. Nestin was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Mouse/Rat Nestin Monoclonal Antibody (Catalog # MAB2736) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Nestin by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Nestin by Immunocytochemistry/Immunofluorescence Zfp322a can enhance OSKM reprogramming and replace Sox2.(A) Zfp322a enhanced reprogramming efficiency and accelerated the onset of reprogramming process. OSKM serves as control experiment. (B) The iPSCs generated from OSKM plus Zfp322a presented alkaline phosphatase activity. There were more AP stained colonies generated from OKSM+Zfp322a compare to OKSM. (C) The iPSCs expressed endogenous Oct4, Nanog, Sox2, Rex1 and SSEA-1, indicating that they were ES-cell like. Immunostaining using anti-Oct4, anti-Nanog anti-Sox2, anti-Rex1 and anti-SSEA-1 antibodies were performed with GFP+ iPSCs generated from OKSM+Zfp322a. (D) GFP+ iPSCs generated by OKSM+Zfp322a were able to express ectoderm, mesoderm and endoderm lineage markers in the EB formation assay. iPSCs were stained with anti-Nestin, anti-Gata4 and anti-alpha smooth muscle actin (SMA) antibodies and pictures were taken at 60× magnification. DAPI (blue) served as nucleus marker. (E) Zfp322a was able to replace Sox2, but not Oct4 or Klf4 in OSKM reprogramming process. Results from three independent experiments were presented. (F) iPSCs generated from OKM plus Zfp322a were positive with AP staining and more AP positive colonies were observed in OKM+Zfp322a as compared to OKSM. (G) iPSCs generated by OKM plus Zfp322a expressed pluripotency markers Oct4, Nanog, Sox2, Rex1 and SSEA-1. (H) iPSCs derived from OKM+Zfp322a could differentiate into ectoderm, mesoderm and endoderm lineages, which were showed by anti-Nestin, anti-Gata4, anti-SMA staining respectively. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pgen.1004038), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nestin

Nestin is a class VI intermediate filament protein (1,2) that is expressed in stem cells of the central nervous system (CNS) (3) but not in mature CNS cells.(4) Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells (5-10). Its transient expression is a critical step in the neural differentiation pathway.(2) Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors (11-13) and hematopoietic progenitors (14).

References
  1. Hockfield, S. and R.D. McKay (1985) J. Neurosci. 5:3310.
  2. Lendahl, U. et al. (1990) Cell 60:585.
  3. Frederiksen, K. and R.D. McKay (1988) J. Neurosci. 8:1144.
  4. Tohyama, T. et al. (1992) Lab. Invest. 66:303.
  5. Uchida, N. et al. (2000) Proc. Natl. Acad. Sci. USA 97:14720.
  6. Frederiksen, K. et al. (1988) Neuron 1:439.
  7. Cattaneo, E. et al. (1990) Nature 347:762.
  8. Reynolds, B.A. and S. Weiss (1992) Science 255:1707.
  9. Rietze, R.L. et al. (2001) Nature 412:736.
  10. Carpenter, M.K. et al. (2001) Exp. Neurol. 172:383.
  11. Zulewski, H. et al. (2001) Diabetes 50:521.
  12. Lumelsky, N. et al. (2001) Science 292:1389.
  13. Lechner, A. et al. (2001) Biochem. Biophys. Res. Commun. 293:670.
  14. Shih, C.C. et al. (2001) Blood 98:2412.
Entrez Gene IDs
10763 (Human); 18008 (Mouse); 25491 (Rat)
Alternate Names
FLJ21841; NES; Nestin

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Citations for Mouse/Rat Nestin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

27 Citations: Showing 1 - 10
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  1. Generation of induced pluripotent stem cell lines from two unrelated individuals with familial hypertrophic cardiomyopathy carrying the MYBPC3 missense c.1484G>A mutation
    Authors: Ribeiro, M;Martins, S;Carvalho, T;Furtado, M;Sampaio Cabral, J;Brito, D;Carmo-Fonseca, M;da Rocha, ST;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Clozapine Increases Nestin Concentration in the Adult Male Rat Hippocampus: A Preliminary Study
    Authors: H Kayir, BW Jenkins, B Alural, JY Khokhar
    International Journal of Molecular Sciences, 2022-03-22;23(7):.
    Species: Mouse
    Sample Types: Tissue Lysates
    Applications: Western Blot
  3. Generation and characterization of induced pluripotent stem cell line (IBBISTi004-A) from an Angelman syndrome patient carrying a class II deletion of the maternal chromosome 15q11.2-q13
    Authors: C Maranga, C Pereira, AC Raposo, A Vieira, S Duarte, EP Bekman, I Milagre, ST da Rocha
    Stem Cell Research, 2022-03-21;61(0):102757.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Murine cerebral organoids develop network of functional neurons and hippocampal brain region identity
    Authors: Francesca Ciarpella, Raluca Georgiana Zamfir, Alessandra Campanelli, Elisa Ren, Giulia Pedrotti, Emanuela Bottani et al.
    iScience
  5. Transplantable human motor networks as a neuron-directed strategy for spinal cord injury
    Authors: Zachary T. Olmsted, Cinzia Stigliano, Annalisa Scimemi, Tatiana Wolfe, Jose Cibelli, Philip J. Horner et al.
    iScience
  6. Specific Increase in Joint Neutrophil Extracellular Traps and Its Relation to Interleukin 6 in Autoimmune Arthritis
    Authors: A Ohyama, A Osada, H Kawaguchi, I Kurata, T Nishiyama, T Iwai, A Ishigami, Y Kondo, H Tsuboi, T Sumida, I Matsumoto
    International Journal of Molecular Sciences, 2021-07-16;22(14):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Enriching neural stem cell and anti‐inflammatory glial phenotypes with electrical stimulation after traumatic brain injury in male rats
    Authors: Eunyoung Park, Johnathan G. Lyon, Melissa Alvarado‐Velez, Martha I. Betancur, Nassir Mokarram, Jennifer H. Shin et al.
    Journal of Neuroscience Research
  8. Cytoskeletal Proteins in Myotendinous Junctions of Human Extraocular Muscles
    Authors: Jing-Xia Liu, Fatima Pedrosa Domellöf
    Investigative Opthalmology & Visual Science
  9. Adult kidney stem/progenitor cells contribute to regeneration through the secretion of trophic factors
    Authors: K Tsuji, S Kitamura, Y Sang, K Fukushima, J Wada
    Stem Cell Res, 2020-05-31;46(0):101865.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  10. Localization of phosphotyrosine adaptor protein ShcD/SHC4 in the adult rat central nervous system
    Authors: HN Robeson, HR Lau, LA New, J Lalonde, JN Armstrong, N Jones
    BMC Neurosci, 2019-12-10;20(1):57.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  11. A functional human motor unit platform engineered from human embryonic stem cells and immortalized skeletal myoblasts
    Authors: Marwah Abd Al Samid, Jamie S. McPhee, Jasdeep Saini, Tristan R. McKay, Lorna M. Fitzpatrick, Kamel Mamchaoui et al.
    Stem Cells and Cloning: Advances and Applications
  12. The Sox2 promoter-driven CD63-GFP transgenic rat model allows tracking neural stem cell-derived extracellular vesicles
    Authors: A Yoshimura, N Adachi, H Matsuno, M Kawamata, Y Yoshioka, H Kikuchi, H Odaka, T Numakawa, H Kunugi, T Ochiya, Y Tamai
    Dis Model Mech, 2018-01-30;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  13. Curcumin Increase the Expression of Neural Stem/Progenitor Cells and Improves Functional Recovery after Spinal Cord Injury
    Authors: WS Bang, KT Kim, YJ Seo, DC Cho, JK Sung, CH Kim
    J Korean Neurosurg Soc, 2017-12-29;61(1):10-18.
    Species: Rat
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  14. MUNC18-1 gene abnormalities are involved in neurodevelopmental disorders through defective cortical architecture during brain development
    Authors: N Hamada, I Iwamoto, H Tabata, KI Nagata
    Acta Neuropathol Commun, 2017-11-30;5(1):92.
    Species: Primate
    Sample Types: Whole Cells
    Applications: ICC
  15. An Immortalized Human Dorsal Root Ganglia Cell Line Provides a Novel Context to Study Herpes Simplex Virus Type-1 Latency and Reactivation
    Authors: NM Thellman, C Botting, Z Madaj, SJ Triezenber
    J. Virol., 2017-05-26;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  16. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
    Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
    Oncotarget, 2017-04-25;8(17):28074-28092.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  17. Peripheral blood derived induced pluripotent stem cells (iPSCs) from a female with familial hypertrophic cardiomyopathy
    Authors: SB Ross, ST Fraser, RD Bagnall, C Semsarian
    Stem Cell Res, 2017-03-07;20(0):76-79.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  18. Simultaneous Isolation of Three Different Stem Cell Populations from Murine Skin
    Authors: Maria Fernanda Forni, Aline Ramos Maia Lobba, Alexandre Hamilton Pereira Ferreira, Mari Cleide Sogayar
    PLOS ONE
  19. The PD-1/B7-H1 pathway modulates the natural killer cells versus mouse glioma stem cells.
    Authors: Huang B, Zhan Y, Zong W, Yu C, Li J, Qu Y, Han S
    PLoS ONE, 2015-08-12;10(8):e0134715.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  20. The protein phosphatase activity of PTEN is essential for regulating neural stem cell differentiation.
    Authors: Lyu J, Yu X, He L, Cheng T, Zhou J, Cheng C, Chen Z, Cheng G, Qiu Z, Zhou W
    Mol Brain, 2015-04-18;8(0):26.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  21. Insulin-like growth factor promotes cardiac lineage induction in vitro by selective expansion of early mesoderm.
    Authors: Engels M, Rajarajan K, Feistritzer R, Sharma A, Nielsen U, Schalij M, de Vries A, Pijnappels D, Wu S
    Stem Cells, 2014-06-01;32(6):1493-502.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. Nestin regulates proliferation, migration, invasion and stemness of lung adenocarcinoma.
    Authors: Narita K, Matsuda Y, Seike M, Naito Z, Gemma A, Ishiwata T
    Int J Oncol, 2014-01-24;44(4):1118-30.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  23. Nestin delineates pancreatic cancer stem cells in metastatic foci of NOD/Shi-scid IL2Rgamma(null) (NOG) mice.
    Authors: Matsuda Y, Yoshimura H, Ueda J, Naito Z, Korc M, Ishiwata T
    Am J Pathol, 2014-01-09;184(3):674-85.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC-P
  24. The pleiotrophin-ALK axis is required for tumorigenicity of glioblastoma stem cells.
    Authors: Koyama-Nasu, R, Haruta, R, Nasu-Nishimura, Y, Taniue, K, Katou, Y, Shirahige, K, Todo, T, Ino, Y, Mukasa, A, Saito, N, Matsui, M, Takahashi, R, Hoshino-Okubo, A, Sugano, H, Manabe, E, Funato, K, Akiyama, T
    Oncogene, 2013-05-20;33(17):2236-44.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  25. Murine cytomegalovirus infection of neural stem cells alters neurogenesis in the developing brain.
    Authors: Mutnal MB, Cheeran MC, Hu S
    PLoS ONE, 2011-01-13;6(1):e16211.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  26. VEGF-mediated angiogenesis stimulates neural stem cell proliferation and differentiation in the premature brain.
    Authors: Sun J, Sha B, Zhou W, Yang Y
    Biochem. Biophys. Res. Commun., 2010-02-24;394(1):146-52.
    Species: Rat
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: ICC, Western Blot
  27. Cytoprotection by a naturally occurring variant of ATP5G1 in Arctic ground squirrel neural progenitor cells
    Authors: NS Singhal, M Bai, EM Lee, S Luo, KR Cook, DK Ma
    Elife, 2020-10-14;9(0):.

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Reviews for Mouse/Rat Nestin Antibody

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Mouse/Rat Nestin Antibody
By Anonymous on 10/26/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: iPS cells Species: Mouse
Immunocytochemistry/Immunofluorescence Mouse/Rat Nestin Antibody MAB2736
Mouse/Rat Nestin Antibody
By Anonymous on 09/04/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: epithelial cells Species: Mouse
Immunocytochemistry/Immunofluorescence Mouse/Rat Nestin Antibody MAB2736
Mouse/Rat Nestin Antibody
By Anonymous on 08/19/2019
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Neural stem cells derived from h9 Species: Human

Neural stem cells were fixed in 4%PFA for 15 minutes. The nestin antibody was used at 10ug/ml at 4 degree overnight. Secondary antibody was incubated at room temperature for 1 hour.

Immunocytochemistry/Immunofluorescence Mouse/Rat Nestin Antibody MAB2736