Mouse & Rat Osteopontin (OPN) ELISA Kit - Quantikine
Mouse/Rat Osteopontin (OPN) Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 62 | 67 | 59 |
Mean (pg/mL) | 96 | 236 | 1085 | 116 | 279 | 1076 |
Standard Deviation | 7.3 | 13.8 | 43.4 | 9.8 | 22.5 | 90 |
CV% | 7.6 | 5.8 | 4 | 8.4 | 8.1 | 8.4 |
Recovery
The recovery of mouse OPN spiked to three levels throughout the range of the assay was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Mouse Cell Culture Supernates (n=7) | 98 | 87-106 |
Rat Cell Culture Supernates (n=7) | 93 | 82-107 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: Osteopontin/OPN
Osteopontin (OPN), also known as bone sialoprotein (BSP), is a secreted SIBLING family protein that can be variably modified by O- and N-glycosylation, sulfation, phosphorylation, and transglutamination. OPN is widely expressed and is prominent in mineralized tissues. It inhibits bone mineralization and kidney stone formation and promotes inflammation, cell ad¬hesion, and migration. Its expression is upregulat¬ed during inflammation, obesity, atherosclerosis, cancer, and tissue damage and contributes to the pathophysiology of these conditions. The central region of OPN contains RGD and non-RGD binding sites for multiple integrins. Adjacent to the RGD motif is the sequence SLAYGLR (SVVYGLR in human) which serves as a cryptic binding site for additional integrins: it is masked in full length OPN but is exposed following OPN cleavage by multiple proteases in tumors and sites of tissue injury.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 5 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Mouse/Rat Osteopontin (OPN) Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Use of Local Melatonin with Xenogeneic Bone Graft to Treat Critical-Size Bone Defects in Rats with Osteoporosis: A Randomized Study
Authors: Costa, KLD;Abreu, LF;Tolomei, CB;Eleutério, RG;Basting, R;Balbinot, G;Collares, FM;Lopes, P;Veiga, N;Fernandes, GVO;Peruzzo, DC;
Journal of functional biomaterials
Species: Rat
Sample Types: Cell Culture Supernates
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Tumor cell derived osteopontin and prostaglandin E2 synergistically promote the expansion of myeloid derived suppressor cells during the tumor immune escape phase
Authors: Mamand, DR;Bazaz, S;Mohammad, DK;Saher, O;Wiklander, OPB;Sadeghi, B;Hassan, M;El-Andaloussi, S;Abedi-Valugerdi, M;
International immunopharmacology
Species: Mouse
Sample Types: Serum, Cell Culture Supernates
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Next-Generation SINE Compound KPT-8602 Ameliorates Dystrophic Pathology in Zebrafish and Mouse Models of DMD
Authors: KG English, AL Reid, A Samani, GJF Coulis, SA Villalta, CJ Walker, S Tamir, MS Alexander
Biomedicines, 2022-09-26;10(10):.
Species: Mouse
Sample Types: Serum
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Evaluation of renal injury and function biomarkers, including symmetric dimethylarginine (SDMA), in the rat passive Heymann nephritis (PHN) model
Authors: MJ Coyne, AE Schultze, DJ McCrann, RE Murphy, J Cross, M Strong-Tow, C Drake, R Mack
PLoS ONE, 2022-05-27;17(5):e0269085.
Species: Rat
Sample Types: Urine
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Dysregulated Autophagy and Mitophagy in a Mouse Model of Duchenne Muscular Dystrophy Remain Unchanged Following Heme Oxygenase-1 Knockout
Authors: O Mucha, K Kaziród, P Podkalicka, K Rusin, J Dulak, A ?oboda
International Journal of Molecular Sciences, 2021-12-31;23(1):.
Species: Mouse
Sample Types: Serum
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Lipid-injured hepatocytes release sOPN to improve macrophage migration via CD44 engagement and pFak-NF&kappaB signaling
Authors: X Jiang, F Zhang, X Ji, F Dong, H Yu, M Xue, Y Qiu, F Yang, X Hu, Z Bao
Cytokine, 2021-02-27;142(0):155474.
Species: Human, Mouse
Sample Types: Cell Culture Supernates, Serum
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Praziquantel pharmacotherapy reduces systemic osteopontin levels and liver collagen content in murine schistosomiasis mansoni
Authors: TA Pereira, G Vaz de Mel, E Trindade S, FE Pereira, M Maria de S
International journal for parasitology, 2021-01-23;0(0):.
Species: Mouse
Sample Types: Serum
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Early Reciprocal Effects in a Murine Model of Traumatic Brain Injury and Femoral Fracture
Authors: K Ritter, K Jung, C Dolderer, D Appel, CC Oswald, U Ritz, MKE Schäfer
Mediators of Inflammation, 2021-01-18;2021(0):8835730.
Species: Mouse
Sample Types: Plasma
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Evaluation of urinary biomarkers for early detection of acute kidney injury in a rat nephropathy model
Authors: K Kohl, E Herzog, G Dickneite, S Pestel
J Pharmacol Toxicol Methods, 2020-08-07;0(0):106901.
Species: Rat
Sample Types: Urine
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Glycation and Serum Albumin Infiltration Contribute to the Structural Degeneration of Bioprosthetic Heart Valves
Authors: A Frasca, Y Xue, AP Kossar, S Keeney, C Rock, A Zakharchen, M Streeter, RC Gorman, JB Grau, I George, JE Bavaria, A Krieger, DA Spiegel, RJ Levy, G Ferrari
JACC Basic Transl Sci, 2020-08-05;5(8):755-766.
Species: Rat
Sample Types: Plasma
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Liver osteopontin is required to prevent the progression of age-related nonalcoholic fatty liver disease
Authors: B Gómez-Sant, D Saenz de U, M Nuñez-Garc, F Gonzalez-R, X Buque, I Aurrekoetx, V Gutiérrez, MJ Gonzalez-R, C García-Mon, Á González-R, L Mosteiro, G Errazti, P Mifsut, S Gaztambide, L Castaño, C Martin, R Nogueiras, ML Martinez-C, WK Syn, P Aspichueta
Aging Cell, 2020-07-07;0(0):e13183.
Species: Mouse
Sample Types: Cell Culture Supernates
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Enhancer and super-enhancer dynamics in repair after ischemic acute kidney injury
Authors: J Wilflingse, M Willi, HK Lee, H Olauson, J Jankowski, T Ichimura, R Erben, MT Valerius, L Hennighaus, JV Bonventre
Nat Commun, 2020-07-07;11(1):3383.
Species: Mouse
Sample Types: Plasma
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The effects of macrophages on cardiomyocyte calcium-handling function using in vitro culture models
Authors: PG Hitscheric, LH Xie, D Del Re, EJ Lee
Physiol Rep, 2019-07-01;7(13):e14137.
Species: Mouse
Sample Types: Cell Culture Supernates
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Pre-clinical evaluation of Minnelide as a therapy for acute myeloid leukemia
Authors: B Giri, VK Gupta, B Yaffe, S Modi, P Roy, V Sethi, SP Lavania, SM Vickers, V Dudeja, S Banerjee, J Watts, A Saluja
J Transl Med, 2019-05-20;17(1):163.
Species: Human
Sample Types: Whole Cells
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Osteopontin in the host response to Leishmania amazonensis
Authors: E Giraud, E Rouault, L Fiette, JH Colle, D Smirlis, E Melanitou
BMC Microbiol., 2019-02-08;19(1):32.
Species: Mouse
Sample Types: Cell Culture Supernates
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Osteopontin potentiates PM-induced IL-1? and IL-1? production via the ERK/JNK signaling pathway
Authors: J Wang, J Huang, M Zhu, S Chen, C Chen, C Miao, H He, Y Song
Ecotoxicol. Environ. Saf., 2019-01-09;171(0):467-474.
Species: Mouse
Sample Types: Serum
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Elevated extracellular calcium ions promote proliferation and migration of mesenchymal stem cells via increasing osteopontin expression
Authors: MN Lee, HS Hwang, SH Oh, A Roshanzade, JW Kim, JH Song, ES Kim, JT Koh
Exp. Mol. Med., 2018-11-05;50(11):142.
Species: Mouse
Sample Types: Cell Culture Supernates
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An osteopontin/CD44 immune checkpoint controls CD8+ T cell activation and tumor immune evasion
Authors: JD Klement, AV Paschall, PS Redd, ML Ibrahim, C Lu, D Yang, E Celis, SI Abrams, K Ozato, K Liu
J. Clin. Invest., 2018-11-05;0(0):.
Species: Mouse
Sample Types: Serum
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Multi-compartment scaffold fabricated via 3D-printing as in vitro co-culture osteogenic model
Authors: E De Giglio, MA Bonifacio, AM Ferreira, S Cometa, ZY Ti, A Stanzione, K Dalgarno, P Gentile
Sci Rep, 2018-10-11;8(1):15130.
Species: Mouse
Sample Types: Cell Culture Supernates
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Atorvastatin provides a new lipidome improving early regeneration after partial hepatectomy in osteopontin deficient mice
Authors: M Nuñez-Garc, B Gomez-Sant, D Saenz de U, D Mestre, F Gonzalez-R, X Buque, V Gutiérrez-, ML Martinez-C, WK Syn, O Fresnedo, P Aspichueta
Sci Rep, 2018-10-02;8(1):14626.
Species: Mouse
Sample Types: Serum
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Disruption of renal arginine metabolism promotes kidney injury in hepatorenal syndrome
Authors: ZV Varga, K Erdelyi, J Paloczi, R Cinar, ZK Zsengeller, T Jourdan, C Matyas, NT Balazs, A Guillot, X Xiang, A Mehal, G Hasko, IE Stillman, S Rosen, B Gao, G Kunos, P Pacher
Hepatology, 2018-10-01;0(0):.
Species: Mouse
Sample Types: Serum
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Tumor-independent host secretomes induced by angiogenesis and immune-checkpoint inhibitors
Authors: M Mastri, CR Lee, A Tracz, RS Kerbel, M Dolan, Y Shi, JML Ebos
Mol. Cancer Ther., 2018-04-25;0(0):.
Species: Mouse
Sample Types: Plasma
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Loss of NF-?B1 Causes Gastric Cancer with Aberrant Inflammation and Expression of Immune Checkpoint Regulators in a STAT-1-Dependent Manner
Authors: LA O'Reilly, TL Putoczki, LA Mielke, JT Low, A Lin, A Preaudet, MJ Herold, K Yaprianto, L Tai, A Kueh, G Pacini, RL Ferrero, R Gugasyan, Y Hu, M Christie, S Wilcox, R Grumont, MDW Griffin, L O'Connor, GK Smyth, M Ernst, P Waring, S Gerondakis, A Strasser
Immunity, 2018-03-20;48(3):570-583.e8.
Species: Mouse
Sample Types: Serum
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In vivo inhibition of nuclear factor of activated T-cells leads to atherosclerotic plaque regression in IGF-II/LDLR-/-ApoB100/100mice
Authors: F Blanco, SE Heinonen, E Gurzeler, LM Berglund, AM Dutius And, O Kotova, AC Jönsson-Ry, S Ylä-Herttu, MF Gomez
Diab Vasc Dis Res, 2018-03-02;0(0):1479164118759.
Species: Mouse
Sample Types: Plasma
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Nitric oxide-sensitive guanylyl cyclase stimulation improves experimental heart failure with preserved ejection fraction
Authors: N Wilck, L Markó, A Balogh, K Kräker, F Herse, H Bartolomae, IA Szijártó, M Gollasch, N Reichhart, O Strauss, A Heuser, D Brockschni, A Kretschmer, R Lesche, F Sohler, JP Stasch, P Sandner, FC Luft, DN Müller, R Dechend, N Haase
JCI Insight, 2018-02-22;3(4):.
Species: Rat
Sample Types: Serum
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Involvement of the accumbal osteopontin-interacting transmembrane protein 168 in methamphetamine-induced place preference and hyperlocomotion in mice
Authors: K Fu, Y Miyamoto, K Otake, K Sumi, E Saika, S Matsumura, N Sato, Y Ueno, S Seo, K Uno, SI Muramatsu, A Nitta
Sci Rep, 2017-10-12;7(1):13084.
Species: Mouse
Sample Types: Cell Culture Supernates
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Uremia does not affect neointima formation in mice
Authors: A Aarup, CH Nielsen, LS Bisgaard, I Bot, HH El-Ali, A Kjaer, LB Nielsen, TX Pedersen
Sci Rep, 2017-07-26;7(1):6496.
Species: Mouse
Sample Types: Plasma
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Soluble bone-derived osteopontin promotes migration and stem-like behavior of breast cancer cells
Authors: GM Pio, Y Xia, MM Piaseczny, JE Chu, AL Allan
PLoS ONE, 2017-05-12;12(5):e0177640.
Species: Mouse
Sample Types: Cell Culture Supernates
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Deficient TSC1/TSC2-complex suppression of SOX9-osteopontin-AKT signalling cascade constrains tumour growth in tuberous sclerosis complex
Authors: Fuquan Jin
Hum. Mol. Genet, 2017-01-15;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
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Ultrastructural changes during osteogenic differentiation in mesenchymal stromal cells cultured in alginate hydrogel
Authors: J Grzesiak, A ?mieszek, K Marycz
Cell Biosci, 2017-01-03;7(0):2.
Species: Rat
Sample Types: Cell Culture Supernates
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The P2X4 receptor is required for neuroprotection via ischemic preconditioning
Sci Rep, 2016-05-13;6(0):25893.
Species: Mouse
Sample Types: Tissue Homogenates
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Age-associated vascular inflammation promotes monocytosis during atherogenesis
Aging Cell, 2016-05-02;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
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High Sodium-Induced Oxidative Stress and Poor Anticrystallization Defense Aggravate Calcium Oxalate Crystal Formation in Rat Hyperoxaluric Kidneys.
Authors: Huang H, Ma M
PLoS ONE, 2015-08-04;10(8):e0134764.
Species: Rat
Sample Types: Urine
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Nuclear factor of activated T cells is activated in the endothelium of retinal microvessels in diabetic mice.
Authors: Zetterqvist A, Blanco F, Ohman J, Kotova O, Berglund L, de Frutos Garcia S, Al-Naemi R, Wigren M, McGuire P, Gonzalez Bosc L, Gomez M
J Diabetes Res, 2015-03-31;2015(0):428473.
Species: Mouse
Sample Types: Plasma
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PHEX mimetic (SPR4-peptide) corrects and improves HYP and wild type mice energy-metabolism.
Authors: Zelenchuk L, Hedge A, Rowe P
PLoS ONE, 2014-05-19;9(5):e97326.
Species: Mouse
Sample Types: Serum
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Phenotypic modulation of primary vascular smooth muscle cells by short-term culture on micropatterned substrate.
Authors: Chang S, Song S, Lee J, Yoon J, Park J, Choi S, Park J, Choi K, Choi C
PLoS ONE, 2014-02-04;9(2):e88089.
Species: Rat
Sample Types: Cell Culture Supernates
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The level of hepatic ABCC6 expression determines the severity of calcification after cardiac injury.
Authors: Brampton C, Aherrahrou Z, Chen L, Martin L, Bergen A, Gorgels T, Erdmann J, Schunkert H, Szabo Z, Varadi A, Le Saux O
Am J Pathol, 2014-01-01;184(1):159-70.
Species: Mouse
Sample Types: Tissue Homogenates
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Plasma membrane Pdia3 and VDR interact to elicit rapid responses to 1alpha,25(OH)(2)D(3).
Authors: Chen J, Doroudi M, Cheung J, Grozier A, Schwartz Z, Boyan B
Cell Signal, 2013-07-27;25(12):2362-73.
Species: Mouse
Sample Types: Cell Culture Supernates
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Osteopontin induces growth of metastatic tumors in a preclinical model of non-small lung cancer.
Authors: Shojaei F, Scott N, Kang X, Lappin PB, Fitzgerald AA, Karlicek S, Simmons BH, Wu A, Lee JH, Bergqvist S, Kraynov E
J. Exp. Clin. Cancer Res., 2012-03-23;31(0):26.
Species: Mouse
Sample Types: Serum
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Mineralocorticoid receptor-mediated DNA damage in kidneys of DOCA-salt hypertensive rats.
Authors: Schupp N, Kolkhof P, Queisser N, Gartner S, Schmid U, Kretschmer A, Hartmann E, Oli RG, Schafer S, Stopper H
FASEB J., 2010-12-06;25(3):968-78.
Species: Rat
Sample Types: Tissue Homogenates
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Protein-disulfide isomerase-associated 3 (Pdia3) mediates the membrane response to 1,25-dihydroxyvitamin D3 in osteoblasts.
Authors: Chen J, Olivares-Navarrete R, Wang Y
J. Biol. Chem., 2010-09-15;285(47):37041-50.
Species: Mouse
Sample Types: Cell Culture Supernates
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Neutralization of osteopontin inhibits obesity-induced inflammation and insulin resistance.
Authors: Kiefer FW, Zeyda M, Gollinger K, Pfau B, Neuhofer A, Weichhart T, Saemann MD, Geyeregger R, Schlederer M, Kenner L, Stulnig TM
Diabetes, 2010-01-27;59(4):935-46.
Species: Mouse
Sample Types: Plasma
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Therapeutic silencing of an endogenous gene by siRNA cream in an arthritis model mouse.
Authors: Takanashi M, Oikawa K, Sudo K, Tanaka M, Fujita K, Ishikawa A, Nakae S, Kaspar RL, Matsuzaki M, Kudo M, Kuroda M
Gene Ther., 2009-05-28;16(8):982-9.
Species: Mouse
Sample Types: Serum
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Heterozygous disruption of the PTEN promotes intestinal neoplasia in APCmin/+ mouse: roles of osteopontin.
Authors: Shao J, Washington MK, Saxena R, Sheng H
Carcinogenesis, 2007-08-11;28(12):2476-83.
Species: Rat
Sample Types: Cell Culture Supernates
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LPS-induced biomarkers in mice: a potential model for identifying insulin sensitizers.
Authors: Briscoe CP, Looper D, Tran P, Herrera J, McDonnell SR, Bhat BG
Biochem. Biophys. Res. Commun., 2007-07-10;361(1):140-5.
Species: Mouse
Sample Types: Plasma
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