Mouse RGM-B Antibody

RGM-B in Mouse Brain. RGM-B was detected in perfusion fixed frozen sections of mouse brain (trigeminal ganglia) using 1.7 µg/mL Sheep Anti-Mouse RGM-B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3597) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of RGM-B by Immunohistochemistry Subregion-specific expression of RGMs and Neogenin in the hippocampus.In situ hybridization (A–C, J–L) and immunohistochemistry (D–I, M–O) on coronal mouse brain sections at E16.5 (A–I) and P5 (J–O). Sections in D–I and M–O are counterstained in blue with fluorescent Nissl. (A–F) RGMa mRNA and protein are expressed in the ventricular zone (VZ), dentate gyrus (DG) and cornu ammonis (CA) region. Strong expression of RGMb mRNA and protein is detected in the pial surface lining the hippocampal fissure (HF). Neogenin transcripts and protein are widely expressed in the developing hippocampus (Hip). (G–I) Immunostaining with isotype matched controls. (J–L) In situ hybridization at P5 shows strong but differential expression patterns of RGMa, RGMb and Neogenin in the CA pyramidal cell layers (Pyr). In addition, strong expression of Neogenin is detected in the granular layer (GC) of the DG. (M–O) Immunohistochemistry reveals expression of RGMa and weak expression of RGMb in the stratum lacunosum moleculare (SLM) and fimbria (FIM). Neogenin strongly labels different hippocampal layers. CX, cortex; Hb, habenula; HC, hippocampal commissure; PO, polymorph layer; SO, stratum oriens; SR, stratum radiatum; Th, thalamus. Scale bar A–C: 400 µm, D–F: 300 µm, G–I: 300 µm, J–L: 500 µm and M–O: 400 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23457482), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of RGM-B by Immunohistochemistry Differential expression of RGMs and Neogenin in the cerebellum.In situ hybridization (A–C, G–I, M–O) and immunohistochemistry (D–F, J–L, P–R) on coronal mouse brain sections at E16.5 (A–F), P5 (G–L) and in the adult (M–R). (A–I, M–O) In situ hybridization and immunohistochemistry reveals strong and broad expression of RGMa, RGMb and Neogenin in the cerebellum (CB). (J–L) Immunostaining shows expression of RGMa and Neogenin in all cerebellar layers at P5. RGMb is expressed in the internal granular layer (IGL), Purkinje cell layer (PCL) and external granular layer (EGL). (P–R) In the adult, RGMa, RGMb and Neogenin protein are expressed in Purkinje cells (PCs) and axons in the granular cell layer (GCL), PCL and molecular layer (ML). Neogenin strongly labels PC dendrites in the ML. DCN, deep cerebellar nuclei; WM, white matter; VZ, ventricular zone. Scale bar A–C: 300 µm, D–F: 250 µm, G–I: 150 µm, J–L: 150 µm, M–O: 100 µm and P–R: 50 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23457482), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of RGM-B by Immunohistochemistry RGMa, RGMb and Neogenin display partially complementary patterns of expression in the developing cortex and on cortical projections.In situ hybridization (A–C′) and immunohistochemistry (D–L) on coronal mouse brain sections at E16.5. Panels A′–C′ and D′–F′ show higher magnifications of the boxed areas in A–C and D–F, respectively. Sections in D–L are counterstained in blue with fluorescent Nissl. (A–C′) In situ hybridization reveals strong expression of RGMa and Neogenin, and moderate expression of RGMb, in the embryonic mouse cortex. Arrow in A indicates neurons of the lateral migratory stream. (D–I) RGMa and Neogenin protein are strongly expressed in the cortex and on various cortical axon projections. Strong staining for RGMb (E), and Neogenin (F), is detected in the corpus callosum (CC). The internal capsule (IC) stains strongly for RGMa (G). (J–L) Immunostaining with isotype-matched control antibodies did show significant staining. CP, cortical plate; FIM, fimbria; IZ, intermediate zone; LV, lateral ventricle; MZ, marginal zone; SP, subplate; STR, striatum; SVZ, subventricular zone; VZ, ventricular zone. Scale bar A–C 400 µm, A′–C′ 200 µm, D–F 300 µm, D′–F′ 150 µm, G-I 300 µm and J-M 300 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23457482), licensed under a CC-BY license. Not internally tested by R&D Systems.