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Mouse RGM-B Antibody

Catalog #: AF3597
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3597
AF3597-SP
RGM-B in Mouse Brain.
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Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse RGM-B Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse RGM-B in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse (rm) RGM-A and rmRGM-C is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse RGM-B
Gly49-Ser414
Accession # Q7TQ33
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse RGM-B (Catalog # 3597-RG)
Flow Cytometry
2.5 µg/106 cells
Neuro‑2A mouse neuroblastoma cell line
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry RGM-B antibody in Mouse Brain by Immunohistochemistry (IHC-Fr). View Larger

RGM-B in Mouse Brain. RGM-B was detected in perfusion fixed frozen sections of mouse brain (trigeminal ganglia) using 1.7 µg/mL Sheep Anti-Mouse RGM-B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3597) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RGM-B

RGM-B, also known as DRAGON, is a 40 kDa member of the repulsive guidance molecule (RGM) family of GPI-linked neuronal and muscle membrane proteins (1, 2). It is synthesized as a preproprotein that consists of a 48 amino acid (aa) signal sequence, a 367 aa mature region, and a 21 aa C-terminal prosegment (3). RGM-B contains two potential N-linked glycosylation sites and an abbreviated von Willebrand factor domain. Potential proteolytic cleavage within the VWF domain is supported by R&D Systems’ in house data (4). Within the region following the VWF domain, mouse RGM-B shares 49% and 43% aa sequence identity with RGM-A and RGM-C, respectively. It shares 90%, 79%, 92%, and 93% aa sequence identity with bovine, chicken, human, and rhesus macaque RGM-B, respectively. RGM-B is expressed in the developing and adult nervous system, particularly in the dorsal root ganglia and mantle layer of the spinal cord (3-5). In mouse, it shows a complementary, non-overlapping distribution with RGM-A (2-5). RGM-B is also expressed in fetal and adult enteric ganglia and in postnatal intestinal epithelium (6). RGM-B expression has been detected in neuronal cell bodies and proximal axonal segments (4) but is also present on the cell surface, where it interacts homophilically and mediates neuronal adhesion (3). RGM-B additionally functions as a BMP coreceptor. It directly binds BMP-2 and -4 but not other TGF-beta family proteins (7). RGM-B associates with BMP type I (ALK-2, -3, -6) and type II (Activin RIIA, Activin RIIB) receptors and enhances BMP signaling (7).

References
  1. Monnier, P.P. et al. (2002) Nature 419:392.
  2. Schmidtmer, J. and D. Engelkamp (2004) Gene Exp. Patterns 4:105.
  3. Samad, T.A. et al. (2004) J. Neurosci. 24:2027.
  4. Niederkofler, V. et al. (2004) J. Neurosci. 24:808.
  5. Oldekamp, J. et al. (2004) Gene Exp. Patterns 4:283.
  6. Metzger, M. et al. (2005) Dev. Dyn. 234:169.
  7. Samad, T.A. et al. (2005) J. Biol. Chem. 280:14122.
Long Name
Repulsive Guidance Molecule B
Entrez Gene IDs
285704 (Human); 68799 (Mouse)
Alternate Names
DKFZp434P228; Dragon; DRG11-responsive axonal guidance and outgrowth of neurite; RGM domain family, member B; RGMB; RGM-B

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Citations for Mouse RGM-B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Dragon (RGMb) induces oxaliplatin resistance in colon cancer cells
    Authors: Ying Shi, Xiao-Xiao Huang, Guo-Bin Chen, Ying Wang, Qiang Zhi, Yuan-Sheng Liu et al.
    Oncotarget
  2. Dragon (repulsive guidance molecule b, RGMb) is a novel gene that promotes colorectal cancer growth
    Authors: Ying Shi, Guo-Bin Chen, Xiao-Xiao Huang, Chuan-Xing Xiao, Huan-Huan Wang, Ye-Sen Li et al.
    Oncotarget
  3. Simultaneous binding of Guidance Cues NET1 and RGM blocks extracellular NEO1 signaling
    Authors: Ross A. Robinson, Samuel C. Griffiths, Lieke L. van de Haar, Tomas Malinauskas, Eljo Y. van Battum, Pavol Zelina et al.
    Cell
  4. RGMb is a novel binding partner for PD-L2 and its engagement with PD-L2 promotes respiratory tolerance.
    Authors: Xiao, Yanping, Yu, Sanhong, Zhu, Baogong, Bedoret, Denis, Bu, Xia, Francisco, Loise M, Hua, Ping, Duke-Cohan, Jonathan, Umetsu, Dale T, Sharpe, Arlene H, DeKruyff, Rosemari, Freeman, Gordon J
    J Exp Med, 2014-04-21;211(5):943-59.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Spatiotemporal Expression of Repulsive Guidance Molecules (RGMs) and Their Receptor Neogenin in the Mouse Brain
    Authors: Dianne M. A. van den Heuvel, Anita J. C. G. M. Hellemons, R. Jeroen Pasterkamp
    PLoS ONE

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