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Mouse S100A9 Antibody

Catalog #: MAB2065
11 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2065
MAB2065-SP
Detection of Mouse S100A9 by Western Blot.
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Product Details
Citations (11)
FAQs
Supplemental Products
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse S100A9 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse S100A9 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) S100A4, rmS100A8, rmS100A10, rhS100B, or rhS100P is observed.
Source
Monoclonal Rat IgG2A Clone # 372510
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse S100A9
Met1-Lys113
Accession # P31725
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse S100A9 antibody by Western Blot. View Larger

Detection of Mouse S100A9 by Western Blot. Western blot shows lysates of mouse leukocytes. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse S100A9 Monoclonal Antibody (Catalog # MAB2065) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band was detected for S100A9 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Mouse S100A9 antibody by Simple Western™ View Larger

Detection of Mouse S100A9 by Simple Western™ Simple Western lane view shows lysates of mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for S100A9 at approximately 19 kDa (as indicated) using 10 µg/mL of Rat Anti-Mouse S100A9 Monoclonal Antibody (Catalog # MAB2065) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S100A9

S100A9, also known as calgranulin B or migration inhibitory factor-related protein 14 (MRP14), belongs to the S100 family of calcium binding proteins of the EF-hand type. It is highly expressed in myeloid cells and other cell types including keratinocytes, epithelial cells and endothelial cells. S100A9 is a cytosolic protein that can also be released into the extracellular milieu. It is associated with various inflammatory diseases.

Long Name
S100 Calcium Binding Protein A9
Entrez Gene IDs
6280 (Human); 20202 (Mouse)
Alternate Names
CAGBMigration inhibitory factor-related protein 14; Calgranulin B; calgranulin-B; Calprotectin L1H subunit; CFAGMRP-14,60B8AG; CGLB; L1AG; LIAG; MAC387; MIF; MRP-14; MRP14Leukocyte L1 complex heavy chain; NIF; P14; protein S100-A9; S100 calcium binding protein A9 (calgranulin B); S100 calcium binding protein A9; S100 calcium-binding protein A9 (calgranulin B); S100 calcium-binding protein A9; S100A9

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Citations for Mouse S100A9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. ATG4B/autophagin-1 regulates intestinal homeostasis and protects mice from experimental colitis.
    Authors: Cabrera S, Fernandez AF, Marino G et al.
    Autophagy.
  2. Role of S100A8/A9 for Cytokine Secretion, Revealed in Neutrophils Derived from ER-Hoxb8 Progenitors
    Authors: Y Zhou, J Hann, V Schenten, S Plançon, JL Bueb, F Tolle, S Bréchard
    International Journal of Molecular Sciences, 2021-08-17;22(16):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. A Critical Role of Zinc Importer AdcABC in Group A Streptococcus-Host Interactions During Infection and Its Implications for Vaccine Development
    Authors: N Makthal, K Nguyen, H Do, M Gavagan, P Chandrangs, JD Helmann, RJ Olsen, M Kumaraswam
    EBioMedicine, 2017-06-02;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Interleukin 6 regulates psoriasiform inflammation-associated thrombosis
    JCI Insight, 2016-12-08;1(20):e89384.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. RAGE expression in tumor-associated macrophages promotes angiogenesis in glioma.
    Authors: Chen X, Zhang L, Zhang I, Liang J, Wang H, Ouyang M, Wu S, da Fonseca A, Weng L, Yamamoto Y, Yamamoto H, Natarajan R, Badie B
    Cancer Res, 2014-10-17;74(24):7285-97.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. ID1 promotes breast cancer metastasis by S100A9 regulation.
    Authors: Gumireddy K, Li A, Kossenkov A, Cai K, Liu Q, Yan J, Xu H, Showe L, Zhang L, Huang Q
    Mol Cancer Res, 2014-06-19;12(9):1334-43.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. The calcium-binding protein complex S100A8/A9 has a crucial role in controlling macrophage-mediated renal repair following ischemia/reperfusion.
    Authors: Dessing, Mark C, Tammaro, Alessand, Pulskens, Wilco P, Teske, Gwendoli, Butter, Loes M, Claessen, Nike, van Eijk, Marco, van der Poll, Tom, Vogl, Thomas, Roth, Johannes, Florquin, Sandrine, Leemans, Jaklien
    Kidney Int, 2014-06-18;87(1):85-94.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. The Acute Neutrophil Response Mediated by S100 Alarmins during Vaginal Candida Infections Is Independent of the Th17-Pathway.
    Authors: Yano, Junko, Kolls, Jay K, Happel, Kyle I, Wormley, Floyd, Wozniak, Karen L, Fidel, Paul L J
    PLoS ONE, 2012-09-25;7(9):e46311.
    Species: Mouse
    Sample Types: Vaginal Lavage Fluid, Whole Tissue
    Applications: ELISA Development, IHC-Fr
  9. Proteomic analysis of the role of S-nitrosoglutathione reductase in lipopolysaccharide-challenged mice.
    Proteomics, 2012-06-01;12(12):2024-35.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  10. S100A8/A9 is not involved in host defense against murine urinary tract infection.
    Authors: Dessing MC, Butter LM, Teske GJ
    PLoS ONE, 2010-10-14;5(10):e13394.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  11. Low expression of the IL-23/Th17 pathway in atopic dermatitis compared to psoriasis.
    Authors: Guttman-Yassky E, Lowes MA, Fuentes-Duculan J, Zaba LC, Cardinale I, Nograles KE, Khatcherian A, Novitskaya I, Carucci JA, Bergman R, Krueger JG
    J. Immunol., 2008-11-15;181(10):7420-7.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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