Mouse Serum Amyloid A4 Antibody

Catalog #: AF4276 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4276
AF4276-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Serum Amyloid A4 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Serum Amyloid A4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross‑reactivity with recombinant human Serum Amyloid A4 is observed and less than 1% cross-reactivity with recombinant mouse Serum Amyloid A1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse Serum Amyloid A4
Asp19-Phe130
Accession # P31532

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Serum Amyloid A4

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Mouse Serum Amyloid A4, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serum Amyloid A4

Mouse serum amyloid A protein-4 (SAA4; also SAA5) is a 14 kDa member of the SAA family of proteins. It is a constitutively produced apolipoprotein component of HDL that may mediate HDL-VLDL interactions. Mature mouse SAA4 is 112 amino acids (aa) in length. There are no notable motifs. Although human SAA4 is glycosylated, mouse SAA4 is not. Human SAA4 also shows truncation of its Lys-Lys-Tyr C-terminus. The mouse C-terminus is distantly related with a Glu-Lys-Phe tripeptide, but it is unknown if it is proteolytically processed. Over aa 19‑130, mouse SAA4 shares 57% and 85% aa identity with mature human and rat SAA4, respectively.

Entrez Gene IDs

6291 (Human); 20211 (Mouse); 365245 (Rat)

Alternate Names

C-SAA; C-SAACSAAConstitutively expressed serum amyloid A protein; SAA4; SAA5; serum amyloid A-4 protein; Serum Amyloid A4; serum amyloid A4, constitutive