Mouse SEZ6L Antibody

Catalog #: AF4804
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4804
AF4804-SP

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Product Details

Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse SEZ6L Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse SEZ6L in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) BSRP-A and rmBSRP-C is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse SEZ6L
Glu32-Ser889
Accession # AAH65117

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

View Larger

SEZ6L in Mouse Brain. SEZ6L was detected in perfusion fixed frozen sections of mouse brain (hippocampus) using Sheep Anti-Mouse SEZ6L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4804) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SEZ6L

SEZ6L (Seizure 6-Like protein; also BSRP-B/Brain-specific receptor-like protein B) is a 200-210 kDa member of the SEZ6 family of proteins. It is expressed in the plasma membrane and ER of CNS neurons, particularly cerebellar interneurons plus Purkinje and granule cells. SEZ6L likely regulates Ca++ availability and thus impacts PKC alpha activation and mGluR1 signaling. Mature mouse SEZ6L is a 932 amino acid (aa) type I transmembrane protein. It contains an 866 aa extracellular domain (ECD) (aa 32-897) and a 45 aa cytoplasmic tail. The ECD shows three CUB domains and five short consensus repeats (SCRs). There is one 190-200 kDa splice variant termed BSRP-B that contains a Phe substitution for aa 807-872 of the fifth SCR domain. Over aa 32-889, mouse SEZ6L shares 80% and 97% aa identity with human and rat SEZ6L, respectively.

Long Name

Seizure-related 6 Homolog-like

Entrez Gene IDs

23544 (Human); 56747 (Mouse); 304554 (Rat)

Alternate Names

Acig1; AIG1; Aig1L; BSRP-B; seizure related 6 homolog (mouse)-like; seizure related gene 6-like; SEZ6L

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse SEZ6L Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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