Mouse SLAM/CD150 Biotinylated Antibody

Catalog #: BAF4330 Datasheet / COA / SDS

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BAF4330

All SLAM/CD150 Antibodies

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Mouse SLAM/CD150 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse SLAM/CD150 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human SLAM, recombinant mouse (rm) 2B4/Fc, rmCD48, and rmCD84 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse SLAM/CD150
Thr25-Pro242
Accession # Q9QUM4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse SLAM/CD150 (Catalog # 4330-SL)

Mouse SLAM/CD150 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Mouse SLAM/CD150 Antibody (Catalog # MAB4330)

Standard: Recombinant Mouse SLAM/CD150 Protein, CF (Catalog # 4330-SL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLAM/CD150

The type I transmembrane glycoprotein Signaling Lymphocytic Activation Molecule (SLAM), also known as CD150, is the prototypic member of the SLAM subgroup of the CD2 protein family. CD2 family proteins function as adhesion molecules and modulators of the immune response (1). Mouse SLAM consists of a 218 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 23 aa transmembrane segment, and a 78 aa cytoplasmic domain with three immunoreceptor tyrosine switch motifs (ITSM) (2). Alternate splicing generates an isoform with a substituted cytoplasmic domain (2). Within the ECD, mouse SLAM shares 58% and 83% aa sequence identity with human and rat SLAM, respectively. It is expressed as a 75 kDa molecule of which approximately 30 kDa is N-linked carbohydrate (2). SLAM is expressed on T cells, B cells, thymocytes, macrophages, dendritic cells, platelets, and hematopoietic stem cells (2 - 7). It is upregulated on activated B cells and CD4⁺ and CD8⁺T cells, although it is downregulated on Th2 polarized cells (2, 3, 8). SLAM interacts homophilically with low affinity, and this interaction induces a Th0/Th1 response characterized by clonal expansion, production of IFN-gamma, and increased cytolytic activity of CD8⁺ T cells (2, 3, 9 - 11). SLAM ligation also promotes B cell activation, allergen-induced eosinophil and mast cell activation, and macrophage responsiveness to LPS (4, 8, 12). In humans, SLAM functions as a cellular entry receptor for measles virus (13, 14).

References

Ma, C.S. et al. (2007) Annu. Rev. Immunol. 25:337.
Castro, A.G. et al. (1999) J. Immunol. 163:5860.
Cocks, B.G. et al. (1995) Nature 376:260.
Wang, N. et al. (2004) J. Exp. Med. 199:1255.
Hahm, B. et al. (2004) Virology 323:292.
Nanda, N. et al. (2005) Blood 106:3028.
Kiel, M.J. et al. (2005) Cell 121:1109.
Punnonen, J. et al. (1997) J. Exp. Med. 185:993.
Mavaddat, N. et al. (2000) J. Biol. Chem. 275:28100.
Aversa, G. et al. (1997) J. Immunol. 158:4036.
Mehrle, S. et al. (2008) Mol. Immunol. 45:796.
Wang, N. et al. (2006) Am. J. Respir. Cell Mol. Biol. 35:206.
Tatsuo, H. et al. (2000) Nature 406:893.
Hsu, E.C. et al. (2001) Virology 279:9.

Long Name

Signaling Lymphocytic Activation Molecule

Entrez Gene IDs

6504 (Human); 27218 (Mouse); 102135470 (Cynomolgus Monkey)

Alternate Names

CD 150; CD150; CD150IPO-3; CDw150; IPO-3; signaling lymphocytic activation molecule family member 1; signaling lymphocytic activation molecule; SLAM; SLAMCD150 antigen; SLAMF1