Mouse THSD1 Antibody

Catalog #: AF4784 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4784
AF4784-SP

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Mouse THSD1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse THSD1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross‑reactivity with recombinant human THSD1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse THSD1
Glu25-Asn412
Accession # Q9JM61

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse THSD1 (Catalog # 4784-TH)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: THSD1

Mouse THSD1 (Thrombospondin type-1 domain-containing protein 1), also known as Transmembrane molecule with thrombospondin module (Tmtsp), is a 95 kDa, type I transmembrane protein (1). It is synthesized as a precursor that is 851 amino acids (aa) in length, and has a 24 aa signal sequence, a 388 aa extracellular region, a 21 aa transmembrane region, and a 418 aa cytoplasmic region. The extracellular region contains six potential N-linked glycosylation sites and the thrombospondin type‑1 (TSP1) domain, which is implicated in cell adhesion and migration (1). Also present in the molecule are 9 PKC-phosphorylation sites, 3 Ig-like domains, and a tyrosine-phosphorylation site by its C-terminus (1). Mouse THSD1 shares 78% aa sequence identity with human THSD1. THSD1 is highly expressed in hematopoietic stem cells (HSCs) and progenitor cells, including CD34^-/lowKit⁺Sca-1⁺Lin^- HSCs, CD34⁺Kit⁺Sca-1⁺Lin^- and Lin^-progenitor cells, and to a lesser degree in thymic CD4^-CD8^- cells (1). Expression gradually decreases during T cell differentiation (1). In addition, THSD1 is widely expressed on endothelial cells, with highest expression in the lung (1). THSD1’s ligand and function are unknown, but it is postulated that THSD1 may be involved in the regulation of vasculogenesis and/or angiogenesis through its interaction with its specific ligand (1). THSD1 may function in primitive hematopoietic cells in the bone marrow niche, and through its TSP1 and Ig-like domains, it may play a role in HSC homing to the niche following cell-to-cell contact to maintain hematopoietic stem cell quiescence (1).

References

Takayanagi, S. et al. (2006) Blood 107:4317.

Long Name

Thrombospondin, Type I, Domain Containing 1

Entrez Gene IDs

55901 (Human); 56229 (Mouse); 364630 (Rat)

Alternate Names

thrombospondin type-1 domain-containing protein 1; thrombospondin, type I, domain 1; thrombospondin, type I, domain containing 1,4833423O18Rik; THSD1; TMTSP; TMTSPMGC74971; Transmembrane molecule with thrombospondin module; UNQ3010