Mouse TIMP-4 Antibody

Catalog #: MAB7667 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB7667-SP
MAB7667-100

All TIMP-4 Antibodies

Product Details

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TIMP-4 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TIMP-4 in direct ELISAs.

Source

Recombinant Monoclonal Rat IgG₁ Clone # 866106R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TIMP-4
Met1-Pro224
Accession # Q9JHB3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human/Mouse TIMP‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF974).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse TIMP-4 DuoSet ELISA Kit (Catalog # DY7667-05) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIMP-4

Tissue Inhibitors of Metalloproteinases (TIMPs) are a family of secreted proteins that regulate the activation and proteolytic activity of the zinc enzymes known as Matrix Metalloproteinases (MMPs). There are four known members of the family, TIMP-1, -2, -3, and -4. TIMP-4 is produced by a wide range of tissues, particularly brain, heart, ovary and skeletal muscle (1, 2). Limited studies have shown that TIMP-4 has a tumor suppressive effect against Wilm’s tumor, exhibits negative correlation with glioma maligancy and is found in breast carcinoma cells (3, 5). TIMP-4 inhibits MMP-mediated proteolysis by forming a noncovalent binary complex with the MMP active site through its N-terminal domain. In addition, it binds to the hemopexin-like domain of proMMP-2 through its C-terminal domain in a manner similar to TIMP-2 (6). However, unlike TIMP-2, TIMP-4 does not promote proMMP-2 activation by MT1-MMP (MMP-14) (7). Although TIMP-4 is a potent inhibitor of most MMPs, it is not an effective inhibitor of ADAMs, such as TACE (8, 9).

References

Greene et al. (1996) J. Biol. Chem. 271:30375.
Leco et al. (1997) FEBS Lett. 401:213.
Geliker et al. (2001) Oncogene 20:4337.
Groft et al. (2001) Br. J. Cancer 85:55.
Hurst et al. (2001) Biochem. Biophys. Res. Comm. 281:166.
Bigg et al. (1997) J. Biol. Chem. 272:15496.
Hernandez-Barrantes et al. (2001) Biochem. Biophys. Res. Comm. 281:126.
Amour et al. (1998) FEBS Lett. 435:39.
Liu et al. (1997) J. Biol. Chem. 272:20479.

Long Name

Tissue Inhibitors of Metalloproteinases 4

Entrez Gene IDs

7079 (Human); 110595 (Mouse)

Alternate Names

metalloproteinase inhibitor 4; TIMP metallopeptidase inhibitor 4; TIMP4; TIMP-4; tissue inhibitor of metalloproteinase 4; Tissue inhibitor of metalloproteinases 4