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Mouse TLR2 Antibody

Catalog #: MAB1530
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1530
MAB1530-SP
Detection of TLR2 in Raw264.7 cells by Flow Cytometry.
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Citations (6)
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Mouse TLR2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TLR2 in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant mouse (rm) TLR1, recombinant human (rh) TLR2, rhTLR3, rhTLR4, or rmTLR6.
Source
Monoclonal Rat IgG2B Clone # 203325
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TLR2
Gln25-Leu590
Accession # Q9QUN7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
Raw264.7 cells stimulated with 100 ng/mL LPS overnight
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of TLR2 in Raw264.7 cells by Flow Cytometry. Raw264.7 cells stimulated with 100 ng/mL LPS overnight were stained with Rat Anti-Mouse TLR2 Monoclonal Antibody (Catalog # MAB1530, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Fluorescein-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0104B). View our protocol for Staining Membrane-associated Proteins.

Western Blot Detection of Mouse TLR2 by Western Blot View Larger

Detection of Mouse TLR2 by Western Blot TLR2Ab suppressed the expression of endogenous TLR2 agonists and interaction of TLR2 with HMGB1.(A) Expression of HMGB1 and Hsp70 were reduced in TLR2Ab-treated mice, but not in TLR4Ab group. (B) TLR2Ab and TLR4Ab suppressed its downstream signaling pathway. Data are shown as mean ± SEM (n = 5/group). (C) TLR2Ab inhibited the interaction of TLR2 with HMGB1 as indicated by co-immunoprecipitation and western blot. Data are representatives of two experiments with identical results. (D) TLR2Ab decreased the co-localization of TLR2 and HMGB1 as shown by confocal microscopy. Data are representatives of two experiments with identical results. #p<0.05, ##p<0.01, ###p<0.001 versus saline-treated group; *p<0.05, **p<0.01, ***p<0.001 versus Dox+IgG-treated group. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22808256), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse TLR2 by Western Blot View Larger

Detection of Mouse TLR2 by Western Blot TLR2Ab suppressed the expression of endogenous TLR2 agonists and interaction of TLR2 with HMGB1.(A) Expression of HMGB1 and Hsp70 were reduced in TLR2Ab-treated mice, but not in TLR4Ab group. (B) TLR2Ab and TLR4Ab suppressed its downstream signaling pathway. Data are shown as mean ± SEM (n = 5/group). (C) TLR2Ab inhibited the interaction of TLR2 with HMGB1 as indicated by co-immunoprecipitation and western blot. Data are representatives of two experiments with identical results. (D) TLR2Ab decreased the co-localization of TLR2 and HMGB1 as shown by confocal microscopy. Data are representatives of two experiments with identical results. #p<0.05, ##p<0.01, ###p<0.001 versus saline-treated group; *p<0.05, **p<0.01, ***p<0.001 versus Dox+IgG-treated group. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22808256), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR2

The Toll-like family of molecules are a group of integral membrane proteins that serve as pattern recognition receptors for microbial pathogens (1-4). To date, there are at least eleven mouse and ten human members that activate the innate immune system following exposure to a variety of microbial species (1, 3). All Toll-like receptors (TLRs) are type I transmembrane (TM) proteins that exist either in the plasma membrane or in the membranes of endosomal structures (where they bind intracellular nucleic acids) (3). All TLRs also contain a large number of extracellular leucine-rich repeats (LRRs) and a cytoplasmic tail with a Toll/IL-1 receptor (TIR) domain. Mouse Toll-like receptor-2 (TLR2) is a 97 kDa, 760 amino acid (aa) glycoprotein that contains a 563 aa extracellular region, a 21 aa TM segment, and a 176 aa cytoplasmic domain (5, 6). The extracellular region contains 16 leucine-rich repeats, while the cytoplasmic tail shows one 146 aa TIR domain. The receptor is expressed on a number of cell types including T cells ( alpha beta and gamma δ), monocytes, dendritic cells, neutrophils, B cells, endothelial cells, mast cells, NK cells, macrophages, and hepatocytes (1, 4, 5, 7, 8). TLR2 functions as part of a heterodimeric complex with either TLR1 or TLR6 (1, 3, 4). These complexes recognize lipoproteins and glycolipids from gram-positive and gram-negative bacteria as well as mycoplasma and yeast. TLR2/TLR1 heterodimers recognize triacylated lipopeptides from a variety of microorganisms. The TLR2/TLR6 heterodimer preferentially recognizes diacylated lipopeptides (9). Biglycan is also known to activate TLR2, but the context is unclear (8). Notably, in human, TLR2 also dimerizes with TLR10. But the TLR10 gene in mouse (but not rat) is mutationally inactive, and thus this complex is nonfunctional (10). Upon ligand recognition, TLR2 delivers an activating signal via the associated adapter molecules, MyD88 and TIRAP (1, 11). Activation via TLR2 also results in production of a number of pro-inflammatory cytokines including TNF-alpha, IL-2, IL-6, IL-12, and MIP-2 (1, 3). The extracellular region of mouse TLR2 is 89%, 67%, 81%, and 65% aa identical to the equivalent region in rat, human, hamster, and canine, respectively.

 

References
  1. Wetzler, L. (2003) Vaccine 21:S2/55.
  2. Netea, M. et al. (2004) J. Leukoc. Biol. 75:749.
  3. Dunne, A. and L. O’Neill (2005) FEBS. Lett. 579:3330.
  4. Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
  5. Matsuguchi, T. et al. (2000) Blood 95:1378.
  6. Meng, G. et al. (2005) Immunol. Lett. 98:200.
  7. Flo, T. et al. (2001) J. Leukoc. Biol. 69:474.
  8. Schaefer, L. et al. (2005) J. Clin. Invest. 115:2223.
  9. Akira, S. (2003) Curr. Opin. Immunol. 15:5.
  10. Hasan, U. et al. (2005) J. Immunol. 174:2942.
  11. Yamamoto M. et al. (2002) Nature 420:324.
Long Name
Toll-like Receptor 2
Entrez Gene IDs
7097 (Human); 24088 (Mouse); 310553 (Rat)
Alternate Names
CD282 antigen; CD282; TIL4CD282; TLR2; toll/interleukin 1 receptor-like 4; Toll/interleukin-1 receptor-like protein 4; toll-like receptor 2

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Citations for Mouse TLR2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. The promotion of nephropathy by Porphyromonas gingivalis lipopolysaccharide via toll-like receptors
    Authors: K Kajiwara, S Takata, TT To, K Takara, Y Hatakeyama, S Tamaoki, RP Darveau, H Ishikawa, Y Sawa
    Diabetol Metab Syndr, 2017-09-22;9(0):73.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  2. Expression of toll-like receptor 2 in glomerular endothelial cells and promotion of diabetic nephropathy by Porphyromonas gingivalis lipopolysaccharide.
    Authors: Sawa Y, Takata S, Hatakeyama Y, Ishikawa H, Tsuruga E
    PLoS ONE, 2014-05-16;9(5):e97165.
    Species: Human, Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: IHC
  3. Vaginal epithelial cell-derived S100 alarmins induced by Candida albicans via pattern recognition receptor interactions are sufficient but not necessary for the acute neutrophil response during experimental vaginal candidiasis.
    Authors: Yano J, Palmer G, Eberle K, Peters B, Vogl T, McKenzie A, Fidel P
    2013-12-09;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Adjuvant effect of killed Propionibacterium acnes on mouse peritoneal B-1 lymphocytes and their early phagocyte differentiation.
    Authors: Mussalem JS, Squaiella-Baptistao CC, Teixeira D, Yendo TM, Thies FG, Popi AF, Mariano M, Longo-Maugeri I
    PLoS ONE, 2012-03-20;7(3):e33955.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Regulation of TLR2 expression by prostaglandins in brain glia.
    Authors: Yoon HJ, Jeon SB, Kim IH, Park EJ
    J. Immunol., 2008-06-15;180(12):8400-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  6. Chemoproteomics reveals Toll-like receptor fatty acylation
    Authors: Nicholas M Chesarino, Jocelyn C Hach, James L Chen, Balyn W Zaro, Murugesan VS Rajaram, Joanne Turner et al.
    BMC Biology

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