Mouse TLR2 Antibody Summary
Gln25-Leu590
Accession # Q9QUN7
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse TLR2 by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse TLR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1530) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TLR2 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TLR2
The Toll-like family of molecules are a group of integral membrane proteins that serve as pattern recognition receptors for microbial pathogens (1‑4). To date, there are at least eleven mouse and ten human members that activate the innate immune system following exposure to a variety of microbial species (1, 3). All Toll-like receptors (TLRs) are type I transmembrane (TM) proteins that exist either in the plasma membrane or in the membranes of endosomal structures (where they bind intracellular nucleic acids) (3). All TLRs also contain a large number of extracellular leucine-rich repeats (LRRs) and a cytoplasmic tail with a Toll/IL-1 receptor (TIR) domain. Mouse Toll-like receptor-2 (TLR2) is a 97 kDa, 760 amino acid (aa) glycoprotein that contains a 563 aa extracellular region, a 21 aa TM segment, and a 176 aa cytoplasmic domain (5, 6). The extracellular region contains 16 leucine-rich repeats, while the cytoplasmic tail shows one 146 aa TIR domain. The receptor is expressed on a number of cell types including T cells ( alpha beta and gamma δ), monocytes, dendritic cells, neutrophils, B cells, endothelial cells, mast cells, NK cells, macrophages and hepatocytes (1, 4, 5, 7, 8). TLR2 functions as part of a heterodimeric complex with either TLR1 or TLR6 (1, 3, 4). These complexes recognize lipoproteins and glycolipids from gram-positive and gram-negative bacteria as well as mycoplasma and yeast. TLR2/TLR1 heterodimers recognize triacylated lipopeptides from a variety of microorganisms. The TLR2/TLR6 heterodimer preferentially recognizes diacylated lipopeptides (9). Biglycan is also known to activate TLR2, but the context is unclear (8). Notably, in human, TLR2 also dimerizes with TLR10. But the TLR10 gene in mouse (but not rat) is mutationally inactive, and thus this complex is nonfunctional (10). Upon ligand recognition, TLR2 delivers an activating signal via the associated adapter molecules, MyD88 and TIRAP (1, 11). Activation via TLR2 also results in production of a number of pro-inflammatory cytokines including TNF-alpha, IL-2, IL-6, IL-12, and MIP-2 (1, 3). The extracellular region of mouse TLR2 is 89%, 67%, 81%, and 65% aa identical to the equivalent region in rat, human, hamster and canine, respectively.
- Wetzler, L. (2003) Vaccine 21:S2/55.
- Netea, M. et al. (2004) J. Leukoc. Biol. 75:749.
- Dunne, A. and L. O’Neill (2005) FEBS. Lett. 579:3330.
- Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
- Matsuguchi, T. et al. (2000) Blood 95:1378.
- Meng, G. et al. (2005) Immunol. Lett. 98:200.
- Flo T. et al. (2001) J. Leukoc. Biol. 69:474.
- Schaefer, L. et al. (2005) J. Clin. Invest. 115:2223.
- Akira S. (2003) Curr. Opin. Immunol. 15:5.
- Hasan, U. et al. (2005) J. Immunol. 174:2942.
- Yamamoto M. et al. (2002) Nature 420:324.
Product Datasheets
Citations for Mouse TLR2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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IL-10 Dysregulation Underlies Chemokine Insufficiency, Delayed Macrophage Response, and Impaired Healing in Diabetic Wounds
Authors: Roy R, Zayas J, Mohamed MF et al.
Journal of Investigative Dermatology
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Mycobacterium vaccae protects against glucocorticoid resistance resulting from combined physical and psychosocial trauma in mice
Authors: D Langgartne, M Amoroso, E Kempter, M Kustermann, J Scheurer, CA Lowry, G Strau beta, SO Reber
Brain, Behavior, and Immunity, 2023-02-01;109(0):221-234.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Bacillus subtilis programs the differentiation of intestinal secretory lineages to inhibit Salmonella infection
Authors: Q Hou, J Jia, J Lin, L Zhu, S Xie, Q Yu, Y Li
Cell Reports, 2022-09-27;40(13):111416.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
IL-10 Dysregulation Underlies Chemokine Insufficiency, Delayed Macrophage Response, and Impaired Healing in Diabetic Wounds
Authors: Roy R, Zayas J, Mohamed MF et al.
Journal of Investigative Dermatology
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Hemorrhagic shock augments lung endothelial cell activation: role of temporal alterations of TLR4 and TLR2.
Authors: Li Y, Xiang M, Yuan Y, Xiao G, Zhang J, Jiang Y, Vodovotz Y, Billiar TR, Wilson MA, Fan J
Am. J. Physiol. Regul. Integr. Comp. Physiol., 2009-10-14;297(6):R1670-80.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Minocycline modulates neuroinflammation independently of its antimicrobial activity in staphylococcus aureus-induced brain abscess.
Authors: Kielian T, Esen N, Liu S, Phulwani NK, Syed MM, Phillips N, Nishina K, Cheung AL, Schwartzman JD, Ruhe JJ
Am. J. Pathol., 2007-08-23;171(4):1199-214.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
TLR-2 is upregulated and mobilized to the hepatocyte plasma membrane in the space of Disse and to the Kupffer cells TLR-4 dependently during acute endotoxemia in mice.
Authors: Ojaniemi M, Liljeroos M, Harju K, Sormunen R, Vuolteenaho R, Hallman M
Immunol. Lett., 2005-09-26;102(2):158-68.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: Electron Microscopy, IHC-P, Western Blot -
Treatment with toll‑like receptor 2 inhibitor ortho‑vanillin alleviates lipopolysaccharide‑induced acute kidney injury in mice
Authors: Yuan Peng, Long Liu, Yongfang Wang, Jianyin Yao, Fang Jin, Tao Tao et al.
Experimental and Therapeutic Medicine
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