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Mouse TRANCE/TNFSF11/RANK L Biotinylated Antibody

Catalog #: BAF462
8 Citations Datasheet / COA / SDS
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BAF462
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Citations (8)
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Mouse TRANCE/TNFSF11/RANK L Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TRANCE in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human (rh) TRANCE, recombinant mouse (rm) RANK, rhCD40 Ligand, and rmOPG is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TRANCE (R&D Systems, Catalog # 462-TR)
Arg72-Asp316
Accession # O35235
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # 462-TR)

Mouse TRANCE/TNFSF11/RANK L Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse TRANCE/TNFSF11/RANK L Antibody (Catalog # AF462)

Standard: Recombinant Mouse TRANCE/RANK L/TNFSF11 Protein (Catalog # 462-TR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRANCE/TNFSF11/RANK L

TRANCE (receptor activator of NF-kappa B ligand [RANK L], also called TNF-related activation-induced cytokines, osteoprotegerin ligand [OPGL], and osteoclast differentiation factor [ODF]), is a member of the tumor necrosis factor (TNF) family. In the TNF superfamily nomenclature, TRANCE is referred to as TNFSF11. TRANCE was originally identified as an immediate early gene upregulated by T cell receptor stimulation. The murine TRANCE cDNA encodes a type II transmembrane protein of 316 amino acids with a predicted cytoplasmic domain of 48 amino acids and an extracellular domain of 247 amino acids. The extracellular domain contains two potential N-linked glycosylation sites. Mouse and human TRANCE share 85% amino acid identity. TRANCE is primarily expressed in T cells and T cell rich organs, such as thymus and lymph nodes. The multi-functions of TRANCE include induction of activation of the c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. RANK is the cell surface signaling receptor of TRANCE. RANK has been shown to undergo receptor clustering during signal transduction. Osteoprotegerin, a soluble member of the TNF receptor family which binds TRANCE, is a naturally occurring decoy receptor that counterbalances the effects of TRANCE.

References
  1. Wong, B.R. et al. (1997) J. Biol. Chem. 272:25190.
  2. Anderson, D.M. et al. (1997) Nature 390:175.
  3. Nakagawa, N. et al. (1998) Biochem. Biophys. Res. Commun. 245:382.
  4. Kong, Y-Y. et al. (1999) Nature 397:315.
Long Name
TNF-related Activation-induced Cytokine
Entrez Gene IDs
8600 (Human); 21943 (Mouse); 117516 (Rat); 102137946 (Cynomolgus Monkey)
Alternate Names
CD254 antigen; CD254; ODF; OPGL; OPGLOPTB2; Osteoclast differentiation factor; Osteoprotegerin ligand; RANK L; RANKL; RANKLreceptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; sOdf; TNF-related activation-induced cytokine; TNFSF11; TRANCE; TRANCEODFhRANKL2; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11

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Citations for Mouse TRANCE/TNFSF11/RANK L Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Interleukin (IL)-25 suppresses IL-22-induced osteoclastogenesis in rheumatoid arthritis via STAT3 and p38 MAPK/I&kappaB&alpha pathway
    Authors: HK Min, JY Won, BM Kim, KA Lee, SJ Lee, SH Lee, HR Kim, KW Kim
    Arthritis Research & Therapy, 2020-09-23;22(1):222.
    Species: Human
    Sample Types: Synovial Fluid
  2. Creation of RANKL mutants with low affinity for decoy receptor OPG and their potential anti‐fibrosis activity
    Authors: Yizhou Wang, Timo Michiels, Rita Setroikromo, Ronald van Merkerk, Robbert H. Cool, Wim J. Quax
    The FEBS Journal
  3. Essential Role of Canonical NF-kappa B Activity in the Development of Stromal Cell Subsets in Secondary Lymphoid Organs
    Authors: Dana Bogdanova, Arata Takeuchi, Madoka Ozawa, Yasuhiro Kanda, M. Azizur Rahman, Burkhard Ludewig et al.
    The Journal of Immunology
  4. A Distinct Subset of Fibroblastic Stromal Cells Constitutes the Cortex-Medulla Boundary Subcompartment of the Lymph Node
    Authors: Arata Takeuchi, Madoka Ozawa, Yasuhiro Kanda, Mina Kozai, Izumi Ohigashi, Yoichi Kurosawa et al.
    Frontiers in Immunology
  5. Systemic lentivirus-mediated delivery of short hairpin RNA targeting calcium release-activated calcium channel 3 as gene therapy for collagen-induced arthritis.
    Authors: Liu S, Kiyoi T, Takemasa E, Maeyama K
    J Immunol, 2014-11-24;194(1):76-83.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development
  6. OX40 signals during priming on dendritic cells inhibit CD4 T cell proliferation: IL-4 switches off OX40 signals enabling rapid proliferation of Th2 effectors.
    Authors: Kim MY, Bekiaris V, McConnell FM, Gaspal FM, Raykundalia C, Lane PJ
    J. Immunol., 2005-02-01;174(3):1433-7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  7. IL-17 promotes bone erosion in murine collagen-induced arthritis through loss of the receptor activator of NF-kappa B ligand/osteoprotegerin balance.
    Authors: Lubberts E, van den Bersselaar L, Oppers-Walgreen B, Schwarzenberger P, Coenen-de Roo CJ, Kolls JK, Joosten LA, van den Berg WB
    J. Immunol., 2003-03-01;170(5):2655-62.
    Species: Mouse
    Sample Types: Synovial Fluid
    Applications: ELISA Development
  8. MLO-Y4 osteocyte-like cells support osteoclast formation and activation.
    Authors: Zhao S, Zhang YK, Harris S, Ahuja SS, Bonewald LF
    J. Bone Miner. Res., 2002-11-01;17(11):2068-79.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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