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Mouse TREM-3 Antibody

Catalog #: MAB2224 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2224
MAB2224-SP
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TREM-3 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TREM-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse (rm) TREM-1 or rmTREM-2b is observed.
Source
Monoclonal Rat IgG2A Clone # 295502
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TREM-3
Met1-Pro135 (predicted)
Accession # NP_067382
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse TREM‑3

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TREM-3

TREM-3 is a type I transmembrane protein possessing a single extracellular Ig domain. It associates with the signal adapter molecule, DAP12, and delivers an activating signal. TREM-3 is expressed in macrophage cell lines and at low levels in T cell lines, but not in NK, B cell, or mast cell lines.

Long Name
Triggering Receptor Expressed on Myeloid Cells 3
Entrez Gene IDs
58218 (Mouse)
Alternate Names
BB134760; TREM3; TREM-3

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