Mouse TSG-6 Biotinylated Antibody

Catalog #: BAF2326
1 Citation Datasheet / COA / SDS

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BAF2326

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All TSG-6 Antibodies

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Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TSG-6 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TSG-6 in Western blots. In Western blots, approximately 50% cross-reactivity with recombinant human TSG-6 is observed and less than 1% cross-reactivity with recombinant mouse TSG-14 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TSG-6
Trp18-Leu275
Accession # O08859

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse TSG‑6 (Catalog # 2326-TS)

Immunocytochemistry

5-15 µg/mL

Immersion fixed mouse splenocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSG-6

TSG-6 (TNF-stimulated gene 6; also named TNFIP6) is a secreted, 35‑39 kDa group A member of the LINK-Module superfamily of proteins (1‑4). Mouse TSG-6 is synthesized as a 275 amino acid (aa) precursor. It contains a 17 aa signal sequence and a 258 aa mature region (5, 6). The mature region has an N-terminal link module (aa 36‑129) and a C-terminal CUB (C1s/C1r; urchin embryonic growth factor; BMP1) domain (aa 135‑246). Link modules bind hyaluronan (HA) and participate in extracellular matrix (ECM) assembly (7). Mature mouse TSG-6 shares 97%, 94% and 94% aa identity with rat, human and canine TSG-6, respectively. Cells reported to express TGF-6 include activated fibroblasts, synoviocytes, chondrocytes, neutrophils, proximal tubular epithelium, bronchial epithelium, endothelium, and visceral plus vascular smooth muscle (2, 8). TSG-6 has multiple functions, many of which involve the ECM. It is suggested to stabilize HA‑rich ECM. It does so by serving as an intermediary, or as a link between the individual subunits of the extracellular decameric pentraxin 3 and the surrounding hyaluronan matrix (9). It also provides structure and organization to hyaluronan. This is accomplished by a TSG-6 mediated transfer of an 80‑85 kDa protein subunit from I alpha I (inter-alpha -inhibitor) to HA. I alpha I is a four-component, 225 kDa serine protease inhibitor. It contains a protease inhibitor subunit (bikunin), two independent, accompaning protein chains (HC1 and HC2), and a short chondroitin sulfate linking moiety. TSG-6 is a catalyst for the removal and transient binding of either HC chain. Each chain is subsequently transferred and covalently-linked to the surrounding HA. This provides substance and reinforcement to the ECM (1, 2, 10, 11, 12). This disassembly of I alpha I also leads to free bikunin, which in the “free” state becomes a potent inhibitor of serine proteases (8).

References

Milner, C.M. et al. (2006) Biochem. Soc. Trans. 34:446.
Milner, C.M. and A.J. Day (2003) J. Cell Sci. 116:1863.
Wisnieewski, H-G. and J. Vilcek (2004) Cytokine Growth Factor Rev. 15:129.
Blundell, C.D. et al. (2005) J. Biol. Chem. 280:18189.
Fulop, C. et al. (1997) Gene 202:95.
Fulop, C. et al. (2003) Development 130:2253.
Kohda, D. et al. (1996) Cell 86:767.
Forteza, R. et al. (2007) Am. J. Respir. Cell Mol. Biol. 36:20.
Salustri, A. et al. (2003) Development 131:1577.
Rugg, M.S. et al. (2005) J. Biol. Chem. 280:25674.
Sanggaard, K.W. et al. (2006) Biochemistry 45:7661.
Sanggaard, K.W. et al. (2005) J. Biol. Chem. 280:11936.

Long Name

Tumor Necrosis Factor-stimulated Gene Sequence 6

Entrez Gene IDs

7130 (Human); 21930 (Mouse)

Alternate Names

Hyaluronate-binding protein; TNF alpha-induced protein 6; TNFAIP6; TNF-stimulated gene 6 protein; TSG6; TSG-6; TSG-6tumor necrosis factor alpha-inducible protein 6; TSG6tumor necrosis factor-inducible gene 6 protein; Tumor necrosis factor alpha-induced protein 6; tumor necrosis factor, alpha-induced protein 6; tumor necrosis factor-stimulated gene-6 protein

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Mouse TSG-6 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Hyaluronan Rafts on Airway Epithelial Cells.
Authors: Abbadi A, Lauer M, Swaidani S, Wang A, Hascall V
J Biol Chem, 2015-11-24;291(3):1448-55.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot