Mouse TSLP DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse TSLP. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4
Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990)
Plate Sealers: ELISA Plate Sealers (Catalog # DY992)
Scientific Data
Product Datasheets
Preparation and Storage
Background: TSLP
Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. The activities of mouse TSLP overlap with, but are distinct from, those of mouse IL-7. Both mouse TSLP and IL-7 can co-stimulate growth of thymocytes and mature T cells, and support B lymphopoiesis in long-term cultures of fetal liver cells and bone-marrow cells. Whereas mouse IL-7 facilitates the development of B220+/IgM- pre-B cells, mouse TSLP promotes the development B220+/IgM+ B cells. Human TSLP was reported to preferentially stimulate myeloid cells, inducing the release of T cell-attracting chemokines from monocytes and enhancing the maturation of CD11c+ dendritic cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence. Within the mature region, six of the seven cysteine residues present in the mouse TSLP involved in intramolecular disulfide bond formation are conserved in the human TSLP. Human TSLP shares approximately 43% aa sequence identity with mouse TSLP. By Northern blot analysis, human TSLP expression has been detected in many tissues with the highest expressions in heart, liver, testis and prostate. TSLP signals through a heterodimeric receptor complex that consists of IL-7 R alpha and the TSLP R, a member of the hemopoietin receptor family most closely related to Rgammac.
TSLP R, also named Delta and CRLM-2 (Cytokine Receptor-like Module-2), was originally cloned as a novel type 1 cytokine receptor with similarity to the common gamma chain (gamma c). It was subsequently identified as a subunit of the cellular receptor for the IL-7-like cytokine TSLP and termed TSLP R. TSLP R shows 24% sequence identity with the mouse gc receptor. TSLP R expression is constitutive in immune and hematopoietic cells, but is up-regulated in Th2 cells.
Assay Procedure
GENERAL ELISA PROTOCOL
Plate Preparation
- Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
- Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
- Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
- Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.
Assay Procedure
- Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
- Repeat the aspiration/wash as in step 2 of Plate Preparation.
- Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
- Repeat the aspiration/wash as in step 2 of Plate Preparation.
- Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
- Repeat the aspiration/wash as in step 2.
- Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
- Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
- Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
Citations for Mouse TSLP DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Indeno[1,2,3-cd]pyrene enhances the sensitivity of airway epithelial cells to ferroptosis and aggravates asthma
Authors: Yu, H;Zhang, C;Pan, H;Gao, X;Wang, X;Xiao, W;Yan, S;Gao, Y;Fu, J;Zhou, Y;
Chemosphere
Species: Mouse
Sample Types: Cell Culture Supernates, BALF
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Context-dependent function of TSLP and IL-1beta in skin allergic sensitization and atopic march
Authors: J Segaud, W Yao, P Marschall, F Daubeuf, C Lehalle, B German, P Meyer, P Hener, C Hugel, E Flatter, M Guivarch, L Clauss, SF Martin, M Oulad-Abde, M Li
Nature Communications, 2022-09-01;13(1):4703.
Species: Mouse
Sample Types: Tissue Homogenate
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Lobelia chinensis Extract and Its Active Compound, Diosmetin, Improve Atopic Dermatitis by Reinforcing Skin Barrier Function through SPINK5/LEKTI Regulation
Authors: NJ Park, BG Jo, SK Bong, SA Park, S Lee, YK Kim, MH Yang, SN Kim
International Journal of Molecular Sciences, 2022-08-04;23(15):.
Species: Mouse
Sample Types: Serum
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Co-Application with Tannic Acid Prevents Transdermal Sensitization to Ovalbumin in Mice
Authors: E Izumi, N Tanahashi, S Kinugasa, S Hidaka, N Zaima, T Moriyama
International Journal of Molecular Sciences, 2022-04-01;23(7):.
Species: Mouse
Sample Types: Tissue Homogenates
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Oxidized carbon black nanoparticles induce endothelial damage through C-X-C chemokine receptor 3-mediated pathway
Authors: N Majumder, M Velayutham, D Bitounis, VK Kodali, MH Hasan Mazu, J Amedro, VV Khramtsov, A Erdely, T Nurkiewicz, P Demokritou, EE Kelley, S Hussain
Redox Biology, 2021-10-04;47(0):102161.
Species: Mouse
Sample Types: BALF
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Oxidant-induced epithelial alarmin pathway mediates lung inflammation and functional decline following ultrafine carbon and ozone inhalation co-exposure
Authors: N Majumder, WT Goldsmith, VK Kodali, M Velayutham, SA Friend, VV Khramtsov, TR Nurkiewicz, A Erdely, PC Zeidler-Er, V Castranova, JR Harkema, EE Kelley, S Hussain
Redox Biology, 2021-08-05;46(0):102092.
Species: Mouse
Sample Types: BALF
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Interleukin (IL)-33 is dispensable for Schistosoma mansoni worm maturation and the maintenance of egg-induced pathology in intestines of infected mice
Authors: JPK Mukendi, R Nakamura, S Uematsu, S Hamano
Parasites & vectors, 2021-01-22;14(1):70.
Species: Mouse
Sample Types: Tissue Homogenates
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DeSUMOylase SENP7-Mediated Epithelial Signaling Triggers Intestinal Inflammation via Expansion of Gamma-Delta T Cells
Authors: A Suhail, ZA Rizvi, P Mujagond, SA Ali, P Gaur, M Singh, V Ahuja, A Awasthi, CV Srikanth
Cell Rep, 2019-12-10;29(11):3522-3538.e7.
Species: Mouse
Sample Types: Cell Culture Supernates
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Inhaled delivery of Interferon-lambda restricts epithelial-derived Th2 inflammation in allergic asthma
Authors: J Won, CH Gil, A Jo, HJ Kim
Cytokine, 2019-03-09;119(0):32-36.
Species: Mouse
Sample Types: BALF
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A steroid alkaloid derivative 02F04 upregulates thymic stromal lymphopoietin expression slowly and continuously through a novel Gq/11-ROCK-ERK1/2 signaling pathway in mouse keratinocytes
Authors: Y Weng, J Wang, Z Yang, M Xi, J Duan, C Guo, Y Yin, R Segawa, T Moriya, T Yonezawa, BY Cha, JT Woo, A Wen, N Hirasawa
Cell. Signal., 2019-01-19;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
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Macrophages redirect phagocytosis by non-professional phagocytes and influence inflammation
Nature, 2016-11-07;0(0):.
Species: Mouse
Sample Types: BALF
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Epidermal RAF prevents allergic skin disease
Elife, 2016-07-19;5(0):.
Species: Mouse
Sample Types: Serum
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The Inflammatory Caspases-1 and -11 Mediate the Pathogenesis of Dermatitis in Sharpin-Deficient Mice.
Authors: Douglas T, Champagne C, Morizot A, Lapointe J, Saleh M
J Immunol, 2015-07-27;195(5):2365-73.
Species: Mouse
Sample Types: Cell Culture Supernates
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Protease-activated receptor-2 activation contributes to house dust mite-induced IgE responses in mice.
Authors: Post, Sijranke, Heijink, Irene H, Petersen, Arjen H, de Bruin, Harold G, van Oosterhout, Antoon J, Nawijn, Martijn
PLoS ONE, 2014-03-20;9(3):e91206.
Species: Mouse
Sample Types: Tissue Homogenates
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Regulation of adult hematopoiesis by the a disintegrin and metalloproteinase 10 (ADAM10).
Authors: Weber S, Wetzel S, Prox J, Lehmann T, Schneppenheim J, Donners M, Saftig P
Biochem Biophys Res Commun, 2013-11-15;442(3):234-41.
Species: Mouse
Sample Types: Plasma
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The Toll-like receptor 5 ligand flagellin promotes asthma by priming allergic responses to indoor allergens.
Authors: Wilson, Rhonda H, Maruoka, Shuichir, Whitehead, Gregory, Foley, Julie F, Flake, Gordon P, Sever, Michelle, Zeldin, Darryl C, Kraft, Monica, Garantziotis, Stavros, Nakano, Hideki, Cook, Donald N
Nat Med, 2012-10-14;18(11):1705-10.
Species: Mouse
Sample Types: BALF
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OX40 ligand and programmed cell death 1 ligand 2 expression on inflammatory dendritic cells regulates CD4 T cell cytokine production in the lung during viral disease.
Authors: Wythe SE, Dodd JS, Openshaw PJ, Schwarze J
J. Immunol., 2012-01-20;188(4):1647-55.
Species: Mouse
Sample Types: BALF
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Deletion of activating Fcgamma receptors does not confer protection in murine cryoglobulinemia-associated membranoproliferative glomerulonephritis.
Authors: Guo S, Muhlfeld AS, Wietecha TA, Peutz-Kootstra CJ, Kowalewska J, Yi K, Spencer M, Pichaiwong W, Nimmerjahn F, Hudkins KL, Alpers CE
Am. J. Pathol., 2009-06-15;175(1):107-18.
Species: Mouse
Sample Types: Serum
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Good kit, clear instructions, protocol useful for optimising and consistent results.
