Mouse Urotensin-II R Antibody

Catalog #: MAB9245 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9245-100
MAB9245-SP

Detection of Urotensin-II R in HEK293 Human Cell Line Transfected with Mouse Urotensin-II and eGFP by Flow Cytometry.

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Mouse Urotensin-II R Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Urotensin-II R. Stains mouse Urotensin-II R transfectants but not irrelevant transfectants in flow cytometry.

Source

Monoclonal Rat IgG_2B Clone # 443707

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse Urotensin-II R synthetic peptide

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Urotensin-II R in HEK293 Human Cell Line Transfected with Mouse Urotensin-II and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse Urotensin-II R or (B) irrelevant transfectants and eGFP was stained with Rat Anti-Mouse Urotensin-II R Monoclonal Antibody (Catalog # MAB9245) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB0061). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Urotensin-II R

Urotensin-II R (UR2R) belongs to the G protein-coupled receptor 1 family. UR2R is a high affinity receptor for urotensin-2 and urotensin-2B. The activity of UR2R is mediated by a G protein that activates a phosphatidylinositol-calcium second messenger system. It is most abundantly expressed in the heart and pancreas. Human UR2R has been correlated with cortico-adrenal carcinoma proliferation.

Long Name

Urotensin-II Receptor

Entrez Gene IDs

2837 (Human); 217369 (Mouse); 57305 (Rat)

Alternate Names

G protein-coupled receptor 14; GPR14; G-protein coupled receptor 14; UR-2-R; UR-II-R; urotensin 2 receptor; urotensin II receptor; urotensin-2 receptor; Urotensin-II R; UrotensinIIR; Urotensin-IIR; UTR; UTR2; UTS2R