Mouse VCAM-1/CD106 Alexa Fluor® 700-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: VCAM-1/CD106

VCAM-1 (CD106), an 85-95 kDa member of the immunoglobulin superfamily, is a type I transmembrane glycoprotein expressed by activated endothelial cells and multiple cell types, including osteoblasts, B cells plus plasma cells, select fibroblasts, Kupffer cells, sensory and autonomic neurons, visceral smooth muscle, simple squamous type I alveolar epithelium, and CD133⁺ CD24⁺ renal stem cells. Its expression is typically induced by IL-1 beta, IL-4, TNF-alpha and IFN-gamma. VCAM-1 binds to leukocyte integrins VLA-4, LPAM-1 and CD11c. Over amino acids (aa) 25-698 (the ECD), mouse VCAM-1 shares approximately 75% and 86% aa sequence identity with human and rat VCAM-1, respectively. During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4 interaction is thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation. ELISA techniques have also shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Within the context, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions.

Product Datasheets

Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Related Research Areas

• Acute Myeloid Leukemia
• Adhesion Molecules in Angiogenesis
• Adhesion Molecules on Endothelial Cells
• Adhesion Molecules on VSMC
• Adhesive Interactions in Allergy and Asthma
• Adipose-derived Stem Cell Markers
• Cancer Biomarkers
• Endothelial Cell Differentiation Markers
• Endothelial Cell Markers
• Endothelial Progenitor and Endothelial Cell Markers
• Hematopoietic Stem Cell Markers
• Immunoglobulin Superfamily CAMs
• Mesenchymal Stem Cell Markers
• Mouse Tissue-Resident Macrophage Markers